Abstract
As a special reference of the nuclear cistron rDNA of cyclopoid copepods, we obtained the entire DNA sequence of a single rDNA repeat unit from Paracyclopina nana (Cyclopoida: Cyclopettidae). The genomic organization of P. nana rDNA (7,974 bp, 51.7% of GC) was observed to be 18S–ITS1–5.8S–ITS2–28S–IGS in order. Comparative analyses of the rDNA between P. nana and other copepods showed that the intergenic spacer (IGS) was highly informative and divergent, while other coding regions are relatively conserved. We detected eleven poly(T) tracts in the IGS, demonstrating the high AT content in this region. In addition, many sub-repeat sequence patterns (e.g., AG, AT, GC, CCG, TC) such as microsatellites were identified from the rDNA IGS of P. nana. In this article, we show the first complete sequence of rDNA from the order Cyclopoida, providing a better understanding of molecular characteristics in molecular taxonomy.
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Acknowledgments
We thank Mr. Winson K. Chan for his comments on the manuscript. This work was supported by a grant of Korea Research Foundation funded to Huem Gi Park (KRF-2006-C00699). Jang-Seu Ki was a recipient of a post-doctoral fellowship funded by the Korea Research Foundation Grant (KRF-2007-355-C00059).
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Guest editors: L. Sanoamuang & J.S. Hwang / Copepoda: Biology and Ecology
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Ki, JS., Park, H.G. & Lee, JS. Extensive analysis of nuclear cistron rDNA sequence of Paracyclopina nana (Cyclopoida: Cyclopettidae). Hydrobiologia 666, 3–9 (2011). https://doi.org/10.1007/s10750-010-0563-6
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DOI: https://doi.org/10.1007/s10750-010-0563-6