Abstract
We confirmed the presence and expression of exocytotic membrane proteins: Syntaxin-1, Syntaxin-4, SNAP-23, and SNAP-25 in the rotifers Brachionus calyciflorus, Lecane quadridentata, and Philodina roseola. These proteins were identified by immunohistochemistry and immunoblot analysis using antibodies against Syntaxin-1, Syntaxin-4, SNAP-23, and SNAP-25. The presence of these proteins were observed mainly in regions of the nervous, reproductive, and glandular systems of these rotifer species, which underlines the fundamental role of exocytotic docking and fusion membrane proteins in the vesicular release of secretory proteins. The immunoblot analysis confirmed the expression of Syntaxin-1, Syntaxin-4, and SNAP-23 in the three rotifer studied, but not of SNAP-25. This study contributes to rotifer biology by revealing the presence of conservative exocytotic machinery such as SNAREs proteins in the phylum Rotifera, including members of the Class Bdelloidea the highest taxa of metazoans where sexual reproduction does not exist.
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References
Clemént, P. & E. Wurdak, 1991. Rotifera. In Harrison, F. W. & E. E. Ruppert (eds), Microscopic Anatomy of Invertebrates. Aschelmintes, Vol. 4. Wiley–Liss Inc., New York.
Gerst, J. E., 1999. SNAREs and SNARE regulators in membrane fusion and exocytosis. Cellular and Molecular Life Sciences 55: 707–734.
Hsu, S. M., L. Raine & H. Fanger, 1981. The use of antividin antibody and avidin–biotin peroxidase complex in immunoperoxidase technics. American Journal of Clinical Pathology 75: 816−821.
Kotikova, E. A., 1995. Localization and neuroanatomy of catecholaminergic neurons in some rotifer species. Hydrobiologia 313/314: 123–127.
Kotikova, E. A., 1998. Catecholaminergic neurons in the brain of rotifers. Hydrobiologia 387/388: 135–140.
Kotikova, E. A., O. I. Raikova, M. Reuter & M. K. S. Gustafsson, 2005. Rotifer nervous system visualized by FMRF amide and 5-HT immunocytochemistry and confocal laser scanning microscopy. Hydrobiologia 546: 239–248.
Laemmli, U. K., 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227: 680–685.
Mark Welch, D. B. & M. Meselson, 2000. Evidence for the evolution of bdelloid rotifers without sexual reproduction of genetic exchange. Science 288: 1211–1215.
McElroy, D. A., E. B. Prophet, B. Mills, J. B. Arrington & L. H. Sobin, 1994. Laboratory Methods in Histotechnology. Armed Forces Institute of Pathology, American Registry of Pathology, Washington, DC.
Mitchell, S. J. & T. A. Ryan, 2004. Syntaxin-1A is excluded from recycling synaptic vesicles at nerve terminals. The Journal of Neuroscience 24(20): 4884–4888.
Nichols, H. W., 1973. Growth media-freshwater. In Stein, J. R. (ed.), Handbook of Phycological Methods. Cambridge University Press, Cambridge, MA.
Nogrady, T. & M. Alai, 1983. Cholinergic neurotransmission in rotifers. Hydrobiologia 104: 149–153.
Pérez-Legaspi, I. A. & R. Rico-Martínez, 1998. Effect of temperature and food concentration in two species of littoral rotifers. Hydrobiologia 387/388: 341–348.
Pineda-Rosas, A., G. E. Santos-Medrano, M. F. Zavala-Reynoso & R. Rico-Martínez, 2005. Identification of acetylcholinesterase receptors in rotifera. Hidrobiología 546: 249–253.
Quintanar, J. L. & V. López, 2001. Synaptic proteins of exocytosis in the liver fluke Fasciola hepatica, the rumian tapeworm Moniezia expansa and the nematode Ascaris suum. Scientiae Naturae 4: 5–13.
Quintanar, J. L. & E. Salinas, 2002. Effect of hypothyroidism on synaptosomal-associated protein of 25 kDa and Syntaxin-1 expression in adenohypophyses of rat. Journal of Endocrinological Investigation 25: 754–758.
Quintanar, J. L., E. Salinas & J. A. Reig, 1998. Immunohistochemical demonstration of Syntaxin and SNAP-25 in chromaffin cells of the frog adrenal gland. General and Comparative Endocrinology 111: 119–122.
Raineri, M., 1984. Histochemical investigations of Rotifera Bdelloidea. I. Localization of cholinesterase activity. The Histochemichal Journal 16: 601–616.
Rico-Martínez, R. & M. Silva-Briano, 1993. Contribution to the knowledge of the rotifera of México. Hydrobiologia 255–256: 467–474.
Salinas, E., J. Ventura, L. E. Córdova & J. L. Quintanar, 2004. Presence of SNAP-25 in rat mast cells. Immunology Letters 95: 105–108.
Silva-Briano, M., R. Rico-Martínez & A. Adabache-Ortíz, 2003. Rotíferos, cladóceros y copépodos del estado de Aguascalientes, México. In Barreiro-Güemes, M. T., M. E. Meave Del Castillo, M. Signoret-Poillon & M. G. Figueroa-Torres (eds), Planctología Mexicana. Sociedad Mexicana de Planctología, A.C. México.
US Environmental Protection Agency, 1985. Methods for measuring the acute toxicity of effluents to freshwater and marine organisms. EPA-600/4-85-013. Washington, DC.
Acknowledgments
We thank to Drs. Kalman Kovacs and Robert L.Wallace for reviewing the manuscript and Biol. Karla E. Macias Herrera and Dr. Javier Ventura Juarez for their valuable support in the development of this research. Financial supported by Consejo Nacional de Ciencia y Tecnología through the scholarship number 136453.
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Pérez-Legaspi, I.A., Montoya-Garcia, M.D.R., Quintanar, J.L. et al. Identification of exocytotic membrane proteins in three rotifer species. Hydrobiologia 600, 147–154 (2008). https://doi.org/10.1007/s10750-007-9184-0
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DOI: https://doi.org/10.1007/s10750-007-9184-0