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Growth promotion of Chlorella ellipsoidea by co-inoculation with Brevundimonas sp. isolated from the microalga

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Abstract

Eight bacterial strains identified as P1, P2, Y1, Y2, W1, W2, G, and R were isolated from a long-term laboratory culture of the green alga Chlorella ellipsoidea. Although it is unknown how these bacterial strains have been maintained with the C. ellipsoidea culture, all appeared to promote the growth of C. ellipsoidea. Co-inoculation of each bacterial strain with C. ellipsoidea resulted in 0.5–3 times greater algal growth than that of C. ellipsoidea alone. The most effective bacterium (i.e., strain P1) was selected and further characterized. Biochemical analysis and transmission electron microscopy revealed that strain P1 is closely related to the genus Brevundimonas. Sequence analysis of the 16S rRNA of strain P1 showed 99.9 and 99.4% nucleotide sequence identity to that of B. nasdae and B. vesicularis, respectively. In addition to the growth promotion of C. ellipsoidea by strain P1, the growth of strain P1 was also significantly enhanced by co-culturing with C. ellipsoidea, indicating a symbiotic relationship between the bacterium and alga. Scanning electron microscopy showed the direct adhesion of strain P1 cells to the surface of C. ellipsoidea cells, as well as the development of abundant crinkles on the surface of co-cultured C. ellipsoidea cells.

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References

  • Agrawal, S. C. & Y. S. Sarma, 1982. Effects of nutrients present in bold’s basal medium on the green alga Stigeoclonium pascheri. Folia Microbiologica 27: 131–137.

    PubMed  CAS  Google Scholar 

  • Aksu, Z., Y. Sag & T. Kutsal, 1992. The biosorption of copper (II) by Chlorella vulgaris and Zoogloea ramigera. Environmental Technology 13: 579–586.

    CAS  Google Scholar 

  • Azam, F., T. Fenchel, J. G. Field, J. S. Gray, L. A. Meyer-Reil & F. Thingstad, 1983. The ecological role of water-column microbes in the sea. Marine Ecology Progress Series 10: 257–263.

    Article  Google Scholar 

  • Becker, E. W., 1992. Micro-algae for human and animal consumption. In Borowitzka, M. A. & L. J. Borowitzka (eds), Micro-algal Biotechnology, Cambridge University Press, Cambridge: 222–256.

    Google Scholar 

  • Bell, W. H., J. M. Lang & R. Mitchell, 1974. Selective stimulation of marine bacteria by algal extracellular products. The Biological Bulletin 143: 265–277.

    Article  Google Scholar 

  • Busse, H. J., E. B. M. Denner & W. Lubitz, 1996. Classification and identification of bacteria: current approaches to an old problem. Overview of methods used in bacterial systematics. Journal of Biotechnology 47: 3–38.

    Article  PubMed  CAS  Google Scholar 

  • Butler, A., 1998. Acquisition and utilization of transition metal ions by marine organisms. Science 281: 207–210.

    Article  PubMed  CAS  Google Scholar 

  • Cole, J. J., 1982. Interactions between bacteria and algae in aquatic ecosystems. Annual Review of Ecology and Systematics 13: 291–314.

    Article  Google Scholar 

  • Croft, M. T., A. D. Lawrence, E. Raux-Deery, M. J. Warren & A. G. Smith, 2005. Algae acquire vitamin B12 through a symbiotic relationship with bacteria. Nature 483: 90–93.

    Article  CAS  Google Scholar 

  • Croft, M. T., M. J. Warren & A. G. Smith, 2006. Algae need their vitamins. Eukaryotic Cell 5: 1175–1183.

    Article  PubMed  CAS  Google Scholar 

  • De Pauw, N. & G. Persoone, 1992. Micro-algae for aquaculture. In Borowitzka, M. A. & L. J. Borowitzka (eds), Micro-algal Biotechnology, Cambridge University Press, Cambridge: 197–221.

    Google Scholar 

  • Doucette, G. J., E. R. McGovern & J. A. Babinchak, 1999. Algicidal bacteria active against Gymnodinium breve (Dinophyceae). I. Bacterial isolation and characterization of killing activity. Journal of Phycology 35: 1447–1454.

    Article  Google Scholar 

  • Fukami, K., T. Nishijima & Y. Ishida, 1997. Stimulative and inhibitory effects of bacteria on the growth of microalgae. Hydrobiologia 358: 185–191.

    Article  Google Scholar 

  • Gonzalez, L. E. & Y. Bashan, 2000. Increased Growth of the microalga Chlorella vulgaris when co-immobilized and co-cultured in alginated beads with the plant-growth-promoting bacterium Azospirillum brasilense. Applied and Environmental Microbiology 66: 1527–1531.

    Article  PubMed  CAS  Google Scholar 

  • Gonzalez, L. E., R. O. Cañizares & S. Baena, 1997. Efficiency of ammonia and phosphorus removal from Colombian agroindustrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus. Bioresource Technology 60: 259–262.

    Article  CAS  Google Scholar 

  • Guerrini, F., A. Mazzotti, L. Boni & R. Pistocchi, 1998. Bacterial–algal interactions in polysaccharide production. Aquatic Microbial Ecology 15: 247–253.

    Article  Google Scholar 

  • Johnson, E. A. & G. H. An, 1991. Astaxanthin from microbial sources. Critical Reviews in Biotechnology 11: 297–326.

    Article  CAS  Google Scholar 

  • Jones, A. K., 1982. The interaction of algae and bacteria. In Bull, A. T. & J. H. Slater (eds.), Microbial Interactions and Communities. Academic Press, New York: 189–247.

    Google Scholar 

  • Kang, Y. H., J. D. Kim, B. H. Kim, D. S. Kong & M. S. Han, 2005. Isolation and characterization of a bio-agent antagonistic to diatom, Stephanodiscus hantzschii. Journal of Applied Microbiology 98: 1030–1038.

    Article  PubMed  CAS  Google Scholar 

  • Kawanura, Y., Y. Li, H. S. Liu, X. X. Huang, Z. Y. Li & T. Ezaki, 2001. Bacterial population in Russian space station “Mir”. Microbiology and Immunology 58: 819–828.

    Google Scholar 

  • Keshtacher-Liebson, E., Y. Hadar & Y. Chen, 1995. Oligotrophic bacteria enhance algal growth under iron-deficient conditions. Applied and Environmental Microbiology 61: 2439–2441.

    CAS  Google Scholar 

  • Lau, P. S., N. F. Y. Tam & Y. S. Wong, 1997. Wastewater nutrient (N and P) removal by carrageenan and alginate immobilized Chlorella vulgaris. Environmental Technology 18: 945–951.

    Article  CAS  Google Scholar 

  • Lewis, L. A. & R. M. McCourt, 2004. Green algae and the origin of land plants. American Journal of Botany 91: 1535–1556.

    Google Scholar 

  • Li, Y., Y. Kawamura, N. Fujiwara, T. Naka, H. Liu, X. Huang, K. Kobayashi & T. Ezaki, 2004. Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov., isolated from the Russian space laboratory Mir. International Journal of Systematic and Evolutionary Microbiology 54: 819–825.

    Article  PubMed  CAS  Google Scholar 

  • Lovejoy, C., J. P. Bowman & G. M. Hallegraeff, 1998. Algicidal effects of a novel marine Pseudoalteromonas isolated (Class Proteobacteria, Gamma Subdivision) on harmful algal bloom species of the genera Chatonella, Gymnodinium, and Heterosigma. Applied and Environmental Microbiology 64: 2806–2813.

    PubMed  CAS  Google Scholar 

  • Mitichkin, O. V., 1995. The results of biotechnological experiments on orbital station Mir-1. In Girain, G. A. (ed.), Mir-1 Space Station, A Technical Overview. NPO Energia Ltd.: 1–161.

  • Mouget, J. L., A. Dakhama, M. C. Lavoie & J. De la Noue, 1995. Algal growth enhancement by bacteria: is consumption of photosynthetic oxygen involved? FEMS Microbiology Ecology 18: 35–44.

    Article  CAS  Google Scholar 

  • Novikova, N. D., 2004. Review of the knowledge of microbial contamination of the Russian manned spacecraft. Microbial Ecology 47: 127–132.

    Article  PubMed  CAS  Google Scholar 

  • Riquelme, C. E., 1988. Interaction between microalgae and bacteria in coastal seawater. Doctoral thesis, Kyoto University, Kyoto, 92 pp.

  • Riquelme, C. E., K. Fukami & Y. Ishida, 1988. Effects of bacteria on the growth of a marine diatom, Asterionella glacialis. Bulletin of Japanese Society of Microbial Ecology 3: 29–34.

    Google Scholar 

  • Sakata, T., 1990. Occurrence of marine Saprospira sp. possessing algicidal activity for diatoms. Nippon Suisan Gakkaishi 56: 1165.

    Google Scholar 

  • Sakata, T. & K. Iwamoto, 1995. Isolation of marine algicidal microorganisms on diatom double layer agar plates. Fisheries Science 61: 173–174.

    CAS  Google Scholar 

  • Sambrook, J. & D. W. Russell, 2001. Molecular Cloning: A Laboratory Manual, 3rd edn. CSH Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  • Segers, P., M. Vancanneyt, B. Pot, U. Torck, B. Hoste, D. Dewettinck, E. Falsen, K. Kersters & P. DeVos, 1994. Classification of Pseudomonas diminuta Lesion and Hugh 1954 and Pseudomonas vesicularis Busing, Doll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., respectively. International Journal of Systematic and Evolutionary Microbiology 44: 499–510.

    Article  CAS  Google Scholar 

  • Sly, L. I., T. L. Cox & T. B. Beckenham, 1999. The phylogenetic relationships of Caulobacter, Asticcacaulis and Brevundimonas species and their taxonomic implications. International Journal of Systematic and Evolutionary Microbiology 49: 483–488.

    Google Scholar 

  • Suminto, I. & K. Hirayama, 1996. Effects of bacterial coexistence on the growth of a marine diatom Chaetoceros gracilis. Fisheries Science 62: 40–43.

    CAS  Google Scholar 

  • Tam, N. F. Y., P. S. Lau & Y. S. Wong, 1994. Wastewater inorganic N and P removal by immobilized Chlorella vulgaris. Water Science and Technology 30: 369–374.

    CAS  Google Scholar 

  • Tupas, L. & I. Koike, 1990. Amino acid and ammonium utilization by heterotrophic marine bacteria grown in enriched seawater. Limnology and Oceanography 35: 1145–1155.

    Article  CAS  Google Scholar 

  • Watanabe, K., M. Imase, K. Sasaki, N. Ohmura, H. Saiki & H. Tanaka, 2006. Composition of the sheath produced by the green alga Chlorella sorokiniana. Letters in Applied Microbiology 42: 538–543.

    Article  PubMed  CAS  Google Scholar 

  • Watanabe, K., N. Takihana, H. Aoyagi, S. Hanada, Y. Watanabe, N. Ohmura, H. Saiki & H. Tanaka, 2005. Symbiotic association in Chlorella culture. FEMS Microbiology Ecology 51: 187–196.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgement

We thank Dr. Jung-Hoon Yoon of KRIBB for the phylogentic analysis of B. nasdae and Dr. Hyun Woo Oh of KRIBB for the scanning electron microscopy. This research was supported by a grant (P-2004-06) from Marine Bioprocess Research Center of the Marine Bio 21 Center funded by the Ministry of Maritime Affairs & Fisheries, Republic of Korea.

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  1. Department of Microbiology, Pukyong National University, 599-1, Daeyeon 3-Dong, Nam-Gu, Busan, 608-737, South Korea

    Yunjung Park, Kyoung-Woo Je, Kyungyong Lee, Sang-Eun Jung & Tae-Jin Choi

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  1. Yunjung Park
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  2. Kyoung-Woo Je
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  3. Kyungyong Lee
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Correspondence to Tae-Jin Choi.

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Park, Y., Je, KW., Lee, K. et al. Growth promotion of Chlorella ellipsoidea by co-inoculation with Brevundimonas sp. isolated from the microalga. Hydrobiologia 598, 219–228 (2008). https://doi.org/10.1007/s10750-007-9152-8

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