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Glucose uptake and extracellular polysaccharides (EPS) produced by bacterioplankton from an eutrophic tropical reservoir (Barra Bonita, SP–Brazil)

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Abstract

We have studied the production of polysaccharides by bacterioplankton in an eutrophic tropical reservoir (Barra Bonita, SP–Brazil) through a decay experiment using glucose as carbon source. The temporal evolution was monitored by measuring the total organic carbon and the contents of monosaccharides. The glucose added to the reservoir sample water was consumed at higher rates within the first hours of incubation, and after 30 days 94.4% of the carbon contents were mineralized; 4.2% remained as particulate organic carbon (POC) form and 1.5% as dissolved organic carbon (DOC) form. The production of polysaccharides occurred in two stages: within the first 48 h, there was intense glucose consumption with small POC increment (ca. 16%) and release of small quantities of dissolved polysaccharide. In the second, more intense stage production was accelerated after the 9th day of incubation, with the highest polysaccharide concentration measured on the 20th day. Such formation of polysaccharides was related to the excretion of capsules and sheaths by bacterioplankton, mainly in the senescence of heterotrophic populations, with release of reserve and structural intracellular materials.

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Acknowledgements

The authors thank Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the scholarship and to Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, process no. 99/07766-0) for financing this study. Dr. D. Giroldo for HPLC analysis, Dr. R. M. Antonio for suggestion in the translation and Dr. Osvaldo N. Oliveira Jr. (IFSC-USP) for his critical proofreading of the manuscript.

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  1. Departmento de Hidrobiologia, Universidade Federal de São Carlos, Via Washington Luiz, Km 235, Sao Carlos, SP, 13565-905, Brazil

    Rafael S. Panhota, Irineu Bianchini Jr & Armando A. H. Vieira

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  1. Rafael S. Panhota
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  2. Irineu Bianchini Jr
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  3. Armando A. H. Vieira
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Correspondence to Irineu Bianchini Jr.

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Panhota, R.S., Bianchini, I. & Vieira, A.A.H. Glucose uptake and extracellular polysaccharides (EPS) produced by bacterioplankton from an eutrophic tropical reservoir (Barra Bonita, SP–Brazil). Hydrobiologia 583, 223–230 (2007). https://doi.org/10.1007/s10750-006-0532-2

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