Genetic structure of populations of the mangrove crab Ucides cordatus (Decapoda: Ocypodidae) at local and regional scales
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The crab Ucides cordatus (Decapoda: Ocypodidae) is a species of considerable economic and ecological importance in mangrove areas of the Western Atlantic coast. However, habitat loss, overfishing, and a new infectious disease are causing substantial reductions in local stocks of this species, leading to a pressing need to design efficient management strategies. A crucial step in this design in an understanding of how the genetic variability of U. cordatus is distributed among estuaries throughout its range. In this study we assess the degree of spatial structure in the pattern of genetic variation of U. cordatus over local (estuaries located within 100 km from each other) and geographical scales (estuaries located farther than 2700 km from each other). Ninety individuals were collected from nine estuaries and analyzed using PCR-RFLP and RAPD techniques. The percentage of polymorphic bands within populations ranged from 15% to 46% for RFLP markers and from 40% to 70% for RAPD markers. Our results failed to demonstrate significant geographical structure in the pattern of genetic variation, indicating that populations of U. cordatus are capable of extensive gene flow among estuaries. The implications of these results for the management of U. cordatus populations are discussed.
KeywordsPhylogeography RAPD RFLP AMOVA Nested Clade analysis
The Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) provided research and scholar assistantships to WAB and JFON, respectively. This study was funded by the Unidade Gestora do Fundo Paraná de Ciência e Tecnologia, State of Paraná, and the Serviço Brasileiro de Apoio às Micro e Pequenas Empresas (SEBRAE), State of Sergipe, Brazil. L. Patella, provided laboratory support.
- Beck, M. W., K. L. Heck, K. W. Able, D. L. Childers, D. B. Eggleston, B. M. Gillanders, B. Halpern, C. G. Hays, K. Hoshino, T. J. Minello, R. J. Orth, P. F. Sheridan & M. R. Weinstein, 2001. The identification, conservation, and management of estuarine and marine nurseries for fish and invertebrates. BioScience 51: 633–641.CrossRefGoogle Scholar
- Dyer, R. J., In prep. GenoServer: a server-based approach to the analysis of population genetic data. To be submitted to Molecular Ecology Notes. Available at http://dyer.bio.vcu.edu/Software/amova.shtml.
- Diele, K., 2000. Life history and population structure of the mangrove crab Ucides cordatus (Linnaeus, 1763) (Crustacea, Decapoda Brachuyura) in Northern Brazil. PhD. Thesis, University of Bremen.Google Scholar
- Farnsworth, E. J. & A. M. Ellison, 1997. The global conservation status of mangroves. Ambio 26: 328–334.Google Scholar
- Nei, M., 1973. The theory and estimation of genetic distance. In Morton, N. E. (ed.), Genetic structure of Populations. University of Hawaii, Honolulu, 45–54.Google Scholar
- Nóbrega, R. R. & A. K. Nishida, 2003. Aspectos socioeconômicos e percepção ambiental dos catadores de caranguejo-uçá, Ucides cordatus (L.1763) (Decapoda, Brachyura) do estuário do rio Mamanguape, Nordeste do Brasil. Interciência 28: 36–43.Google Scholar
- Seutin, G., B. N. White & P. T. Boag, 1991. Preservation of avian blood and tissue samples for DNA analyses. Canadian Journal of Zoology–Revue Canadienne de Zoologie 69: 82–90.Google Scholar
- Schneider, S., D. Roessli & L. Excoffier, 2000. Arlequin ver 2.000: A Software for Population Genetics Data Analysis. Genetics and Biometry Laboratory, University of Geneva, Switzerland.Google Scholar