Abstract
Superoxide dismutases (SODs) promote a conversion of harmful reactive oxygen species (ROS) to relatively moderate forms, resulting in the extension of lifespan in the nematode Caenorhabditis elegans under caloric restriction. The lifespan of the rotifer Brachionus plicatilis is also markedly extended by caloric restriction. We, therefore, cloned cDNA encoding SOD activated with Mn (Mn SOD) from B. plicatilis and examined its expression pattern in rotifers raised with energy restricted diet. The full length deduced amino acid sequence of the rotifer Mn SOD showed 61% identity with the C. elegans ortholog. Four amino acid residues that are essential to the binding of this enzyme to Mn were conserved in the rotifer Mn SOD. Subsequently we examined the mRNA expression patterns of Mn SOD using highly sensitive quantitative real-time PCR for various rotifer populations that are likely to differ in their lifespans in experiments on calorie restricted diets. The accumulated mRNA levels of Mn SOD were found to increase in supposedly long-lived rotifers. These results suggest that Mn SOD is possibly related to the aging of B. plicatilis.
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C. C. Chu W. E. Paul (1998) ArticleTitleExpressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis Molecular Immunology 35 487–502 Occurrence Handle10.1016/S0161-5890(98)00031-5 Occurrence Handle9798653
D. DiSilvestre S. R. Kleeberger J. Johns R. C. Levitt (1995) ArticleTitleStructure and DNA sequence of the mouse MnSOD gene Mammalian Genome 6 281–284 Occurrence Handle10.1007/BF00352417 Occurrence Handle7613035
H. E. Enesco (1993) ArticleTitleRotifers in aging research: use of rotifers to test various theories of aging Hydrobiologia 255/256 59–70 Occurrence Handle10.1007/BF00025821
T. Finkel N. J. Holbrook (2000) ArticleTitleOxidants, oxidative stress and the biology of ageing Nature 408 239–247 Occurrence Handle11089981
T. Furuyama T. Nakazawa I. Nakano N. Mori (2000) ArticleTitleIdentification of the differential distribution patterns of mRNAs and consensus binding sequences for mouse DAF-16 homologues Biochemical Journal 349 629–634 Occurrence Handle10.1042/0264-6021:3490629 Occurrence Handle10880363
L. Guarente C. Kenyon (2000) ArticleTitleGenetic pathways that regu- late ageing in model organisms Nature 408 255–262 Occurrence Handle10.1038/35041700 Occurrence Handle11089983
Y. Honda S. Honda (1999) ArticleTitleThe daf-2 gene network for longevity regulates oxidative stress resistance and Mn-superoxide dismutase gene expression in Caenorhabditis elegans FASEB Journal 13 1385–1393 Occurrence Handle10428762
M. Johnston S. Andrews R. Brinkman J. Cooper H. Ding J. Dover Z. Du A. Favello L. Fulton S. Gattung et al. (1994) ArticleTitleComplete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII Science 265 2077–2082 Occurrence Handle8091229
G. Kaneko S. Kinoshita T. Yoshinaga K. Tsukamoto S. Watabe (2002) ArticleTitleChanges in expression patterns of stress protein genes during population growth of the rotifer Brachionus plicatilis Fisheries Science 68 1317–1323 Occurrence Handle10.1046/j.1444-2906.2002.00570.x
C. Leblanc A. Falciatore M. Watanabe C. Bowler (1999) ArticleTitleSemi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatom Plant Molecular Biology 40 1031–1044 Occurrence Handle10.1023/A:1006256300969 Occurrence Handle10527427
C. A. Marres A. P. Loon Particlevan P. Oudshoorn H. Steeg ParticleVan L. A. Grivell E. C. Slater (1985) ArticleTitleSequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron-sulphur protein European Journal of Biochemistry 147 153–161 Occurrence Handle10.1111/j.1432-1033.1985.tb08731.x Occurrence Handle3882422
T. L. Parkes A. J. Elia D. Dickinson A. J. Hilliker J. P. Phillips G. L. Boulianne (1998) ArticleTitleExtension of Drosophila lifespan by overexpression of human SOD1 in motorneurons Nature Genetics 19 171–174 Occurrence Handle10.1038/534 Occurrence Handle9620775
F. Piano A. J. Schetter M. Mangone L. Stein K. J. Kemphues (2000) ArticleTitleRNAi analysis of genes expressed in the ovary of Caenorhabditis elegans Current Biology 10 1619–1622 Occurrence Handle10.1016/S0960-9822(00)00869-1 Occurrence Handle11137018
C. Verdone-Smith H. E. Enesco (1982) ArticleTitleThe effect of temperature and of dietary restriction on lifespan and reproduction in the rotifer Asplanchna brightwelli Experimental Gerontology 17 255–262 Occurrence Handle10.1016/0531-5565(82)90013-4 Occurrence Handle7160443
J. R. Wispe J. C. Clark M. S. Burhans K. E. Kropp T. R. Korfhagen J. A. Whitsett (1989) ArticleTitleSynthesis and processing of the precursor for human manganese-superoxide dismutase Biochimica et Biophysica Acta 994 30–36 Occurrence Handle2462451
T. Yoshinaga A. Hagiwara K. Tsukamoto (1999) ArticleTitleEffect of conditioned media on the asexual reproduction of the monogonont rotifer Brachionus plicatilis O. F. Müller Hydrobiologia 412 103–110 Occurrence Handle10.1023/A:1003860502015
T. Yoshinaga A. Hagiwara K. Tsukamoto (2000) ArticleTitleEffect of periodical starvation on the life history of Brachionus plicatilis O. F. Müller (Rotifera): a possible strategy for population stability Journal of Experimental Marine Biology and Ecology 253 253–260 Occurrence Handle10.1016/S0022-0981(00)00268-9 Occurrence Handle11033368
T. Yoshinaga A. Hagiwara K. Tsukamoto (2003) ArticleTitleLife history response and age-specific tolerance to starvation in Brachionus plicatilis O. F. Müller. Journal of Experimental Marine Biology and Ecology 287 261–271 Occurrence Handle10.1016/S0022-0981(02)00574-9
I. N. Zelko T. J. Mariani R. J. Folz (2002) ArticleTitleSuperoxide dismutase multigene family; a comparison of the Cu/Zn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression Free Radical Biology and Medicine 33 337–349 Occurrence Handle10.1016/S0891-5849(02)00905-X Occurrence Handle12126755
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Kaneko, G., Yoshinaga, T., Yanagawa, Y. et al. Molecular Characterization of Mn-superoxide Dismutase and Gene Expression Studies in Dietary Restricted Brachionus plicatilis Rotifers. Hydrobiologia 546, 117–123 (2005). https://doi.org/10.1007/s10750-005-4107-4
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DOI: https://doi.org/10.1007/s10750-005-4107-4