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Journal of the History of Biology

, Volume 47, Issue 1, pp 63–105 | Cite as

Cancer, Conflict, and the Development of Nuclear Transplantation Techniques

  • Nathan CroweEmail author
Article

Abstract

The technique of nuclear transplantation – popularly known as cloning – has been integrated into several different histories of twentieth century biology. Historians and science scholars have situated nuclear transplantation within narratives of scientific practice, biotechnology, bioethics, biomedicine, and changing views of life. However, nuclear transplantation has never been the focus of analysis. In this article, I examine the development of nuclear transplantation techniques, focusing on the people, motivations, and institutions associated with the first successful nuclear transfer in metazoans in 1952. The conflict between embryologists and geneticists over the mechanisms of differentiation motivated Robert Briggs to pursue nuclear transplantation experiments as a way to resolve the debate. Briggs worked at the Lankenau Hospital Research Institute, a research facility devoted to the study of cancer. The goal of understanding cancer would play a role in the development of the technique, and the story of nuclear transplantation sheds light on the role that biomedical contexts play in biological research in the second half of the twentieth century.

Keywords

Nuclear transplantation Nuclear transfer Cloning Differentiation Embryology Cancer research 

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References

  1. Allen, Garland. 1974. “Opposition to the Mendelian-Chromosome Theory: The Physiological and Developmental Genetics of Richard Goldschmidt.” Journal of the History of Biology 7: 749–792.Google Scholar
  2. Allen, Garland. 1986. “T. H. Morgan and the Split Between Embryology and Genetics, 1910–1935.” T.J. Horder, J.A. Witkowski, and C.C. Wylie (eds.), A History of Embryology. Cambridge: Cambridge University Press, pp. 113–146.Google Scholar
  3. Anderson, T.F. 1975. “Jack Schultz (1904–1971).” Genetics 81: 1–7.Google Scholar
  4. Armon, R. 2012. “Between Biochemists and Embryologists – The Biochemical Study of Embryonic Induction in the 1930s.” Journal of the History of Biology 45: 65–108.CrossRefGoogle Scholar
  5. Aronowitz, Robert A. 2007. Unnatural History: Breast Cancer and American Society. Cambridge: Cambridge University Press.Google Scholar
  6. Bechtel, William. 2006. Discovering Cell Mechanisms: The Creation of Modern Cell Biology. New York: Cambridge University Press.Google Scholar
  7. Briggs, Robert W. 1939. “Changes in the Density of the Frog Embryo (Rana pipiens) During Development.” Journal of Cellular and Comparative Physiology 13: 77–89.CrossRefGoogle Scholar
  8. Briggs, Robert W. 1946. “Effects on the Growth Inhibitor, Hexenolactone, on Frog Embryos. I. Effects of Diploid Embryos. II. Differential Effects on Haploid and Diploid Embryos.” Growth 10: 45–73.Google Scholar
  9. Briggs, Robert W., Green, E.U., and King, Thomas. 1951. “An Investigation of the Capacity for Cleavage and Differentiation in Rana pipiens Eggs Lacking ‘Functional’ Chromosomes.” Journal of Experimental Zoology 116: 455–499.CrossRefGoogle Scholar
  10. Briggs, Robert W., and King, Thomas. 1952. “Transplantation of Living Nuclei from Blastula Cells into Enucleated Frogs’ Eggs.” Proceedings of the National Academy of Sciences 38: 455–463.CrossRefGoogle Scholar
  11. Briggs, Robert W., and King, Thomas. 1953. “Factors Affecting the Transplantability of Nuclei of Frog Embryonic Cells.” Journal of Experimental Zoology 122: 485–505.CrossRefGoogle Scholar
  12. Bud, Robert. 1978. “Strategy in American Cancer Research After World War II: A Case Study.” Social Studies of Science 8: 425–459.CrossRefGoogle Scholar
  13. Bud, Robert. 1993. The Uses of Life: A History of Biotechnology. Cambridge:Cambridge University Press.Google Scholar
  14. Burian, Richard. 2005. “Lillie’s Paradox – Or, Some Hazards of Cellular Geography.” The Epistemology of Development, Evolution, and Genetics: Selected Essays. Cambridge: Cambridge University Press.Google Scholar
  15. Burian, Richard M., Gayon, Jean, and Zallen, Doris T. 1991. “Boris Ephrussi and the Synthesis of Genetics and Embryology.” Scott Gilbert (ed.), A Conceptual History of Modern Embryology. New York: Plenum Press.Google Scholar
  16. Cambrosio, Alberto, Keating, Peter, and Tauber, Alfred I. 1994. “Introduction: Immunology as a Historical Object.” Journal of the History of Biology 27: 375–378.CrossRefGoogle Scholar
  17. Campbell, Keith H.S. 2007. “Ten Years of Cloning: Questions Answered and Personal Reflections.” Cloning and Stem Cells 9: 8–11.CrossRefGoogle Scholar
  18. Canguilhem, Georges. 1991. The Normal and the Pathological. Translated by Carolyn R. Fawcett. New York: Zone Books.Google Scholar
  19. Cantor, David. 2008. Cancer in the Twentieth Century. Baltimore: Johns Hopkins University Press.Google Scholar
  20. Carter, K. Coddell. 2003. The Rise of Causal Concepts of Disease: Case Histories. Hants: Ashgate Publishing.Google Scholar
  21. Comandon, J., and de Fonbrune, P. 1939. “Greffe Nucleaires Totale, Simple ou Multiple, chez une Amibe.” Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 130: 744–748.Google Scholar
  22. Crowe, Nathan. 2011. “A ‘Fantastical’ Experiment: Motivations, Practice, and Conflict in the History of Nuclear Transplantation, 1925–1970.” PhD diss., University of Minnesota.Google Scholar
  23. Davson, Hugh, and Danielli, James Frederic. 1950. The Permeability of Natural Membranes. Cambridge: Cambridge University Press.Google Scholar
  24. Di Berardino, Marie. 1997. Genomic Potential of Differentiated Cells. New York: Columbia University Press.Google Scholar
  25. Di Berardino, Marie. 2011. “Interview by Author.” Digital Recording, Haverford, PA, March 2.Google Scholar
  26. Dietrich, Michael. 2000. “From Hopeful Monsters to Homeotic Effects: Richard Goldschmidt’s Integration of Development, Evolution, and Genetics.” American Zoologist 40: 738–747.CrossRefGoogle Scholar
  27. “Faculty Profile: Leigh Hoadley.” 1941, October 10. The Harvard Crimson. Cambridge. Retrieved from http://www.thecrimson.harvard.edu/article/1941/10/10/faculty-profile-pthe-captain-announced-to/?print=1.
  28. Franklin, Sarah. 2007. Dolly Mixtures: The Remaking of a Genealogy. Durham: Duke University Press.CrossRefGoogle Scholar
  29. Fujimura, Joan H. 1995. “Ecologies of Action: Recombining Genes, Molecularizing Cancer, and Transforming Biology.” Susan Leigh Star (ed.), Ecologies of Knowledge: Work and Politics in Science and Technology. Albany: State University of New York Press.Google Scholar
  30. Fujimura, Joan H. 1996. Crafting Science: A Sociohistory of the Quest for the Genetics of Cancer. Cambridge, MA: Harvard University Press.Google Scholar
  31. “Fundamental Cancer Research – Report of a Committee Appointed by the Surgeon General.” 1957. Journal of the National Cancer Institute 19: 317–328. Reprinted from Public Health Reports 53(1938): 2121–2130.Google Scholar
  32. Gilbert, Scott. 1988. “Cellular Politics: Ernest Everett Just, Richard B. Goldschmidt and the Attempt to Reconcile Embryology and Genetics.” R. Rainger, K. Benson, and J. Maienschein (eds.), The American Development of Biology. New Brunswick: Rutgers University Press.Google Scholar
  33. Gilbert, Scott. 1991. Induction and the Origins of Developmental Genetics. A Conceptual History of Developmental Biology. New York: Plenum Press.Google Scholar
  34. Gilbert, Scott. 1998. “Bearing Crosses: A Historiography of Genetics and Embryology.” American Journal of Medical Genetics 76: 168–182.CrossRefGoogle Scholar
  35. Gorbman, Aubrey. 1979. “Emil Witschi and the Problem of Vertebrate Sexual Differentiation.” American Zoologist 19: 1261–1270.Google Scholar
  36. Gurdon, John. 1962a. “The Developmental Capacity of Nuclei Taken from Intestinal Epithelium Cells of Feeding Tadpoles.” Journal of Embryology and Experimental Morphology 10: 622–640.Google Scholar
  37. Gurdon, John. 1962b. “Adult Frogs Derived from the Nuclei of Single Somatic Cells.” Developmental Biology 4: 256–273.CrossRefGoogle Scholar
  38. Gurdon, John, and Byrne, J.A. 2003. “The First Half-Century of Nuclear Transplantation.” Proceedings of the National Academy of Sciences 100: 8048–8052.CrossRefGoogle Scholar
  39. Gurdon, John, Elsdale, Thomas, and Fischberg, Michel. 1958. “Sexually Mature Individuals of Xenopus laevis from the Transplantation of Single Somatic Nuclei.” Nature 182: 64–65.CrossRefGoogle Scholar
  40. Gurdon, John, and Hopwood, Nick. 2000. “The Introduction of Xenopus laevis into Developmental Biology: Of Empire, Pregnancy Testing and Ribosomal Genes.” The International Journal of Developmental Biology 44: 43–50.Google Scholar
  41. Hamburger, Victor. 1988. The Heritage of Experimental Embryology: Hans Spemann and the Organizer. Oxford: Oxford University Press.Google Scholar
  42. Hammett, Frederick S. 1937. “Introduction.” Growth 1.Google Scholar
  43. Harwood, Jonathan. 1993. Styles of Scientific Thought: The German Genetics Community 1900–1933. Chicago: University of Chicago Press.Google Scholar
  44. Hoadley, Leigh. 1934. “A Device for Micromanipulation.” Science 80(2076): 338–339.CrossRefGoogle Scholar
  45. Hoadley, Leigh. 1950. “Founding of the Society for Study of Development and Growth.” Growth 14.Google Scholar
  46. Horder, T.J., Witkowski, J.A., and Wylie, C.C. (eds.). 1985. A History of Embryology. Cambridge: Cambridge University Press.Google Scholar
  47. Hughes, Sally Smith. 1977. The Virus: A History of the Concept. New York: Science History Publications.Google Scholar
  48. Hughes, Sally Smith. 2011. Genentech: The Beginnings of Biotech. Chicago: University of Chicago Press.CrossRefGoogle Scholar
  49. Jacob, François, and Monod, Jacqués. 1961. “Genetic Regulatory Mechanisms in the Synthesis of Proteins.” Journal of Molecular Biology 3: 318–356.CrossRefGoogle Scholar
  50. Keating, Peter, and Cambrosio, Alberto. 2003. Biomedical Platforms: Realigning the Normal and the Pathological in Late-Twentieth-Century Medicine. Cambridge, MA: MIT Press.Google Scholar
  51. Keating, Peter, and Cambrosio, Alberto. 2004. “Does Biomedicine Entail the Successful Reduction of Pathology to Biology?” Perspectives in Biology and Medicine 47: 357–371.CrossRefGoogle Scholar
  52. Kass, Leon. 2002. Human Cloning and Human Dignity: The Report of the President’s Council on Bioethics. New York: Public Affairs.Google Scholar
  53. Kevles, Daniel. 2003. “Pursuing the Unpopular: A History of Courage, Viruses, and Cancer.” Robert B. Silvers (ed.), Hidden Histories of Science. New York: New York Review of Books, pp. 69–114.Google Scholar
  54. King, Thomas, and Briggs, R.W. 1955. “Changes in the Nuclei of Differentiating Gastrula Cells, as Demonstrated by Nuclear Transplantation.” PNAS 41: 321–325.CrossRefGoogle Scholar
  55. King, Thomas, and Briggs, R.W. 1956. “Serial Transplantation of Embryonic Nuclei.” Cold Spring Harbor Symposia on Quantitative Biology. Genetic Mechanisms: Structure and Function. Cold Spring Harbor, NY: Long Island Biological Association.Google Scholar
  56. King, Thomas, and McKinnell, Robert. 1960. “An Attempt to Determine the Developmental Potentialities of the Cancer Cell Nucleus by Means of Transplantation.” Cell Physiology of Neoplasia. Austin: University of Texas Press, pp. 591–617.Google Scholar
  57. Kohler, Robert E. 1994. Lords of the Fly: Drosophila Genetics and the Experimental Life. Chicago: University of Chicago Press.Google Scholar
  58. Kolata, Gina. 1998. Clone: The Road to Dolly, and the Path Ahead. New York: W. Morrow & Co.Google Scholar
  59. Kreeger, Karen. 2004. “America’s First Cancer Center Celebrates Centennial.” Journal of the National Cancer Institute 96: 171–172.CrossRefGoogle Scholar
  60. Landecker, Hannah. 2007. Culturing Life: How Cells Become Technologies. Cambridge: Harvard University Press.Google Scholar
  61. Laubichler, Manfred D., and Davidson, Eric H. 2008. “Boveri’s Long Experiment: Sea Urchin Merogones and the Establishment of the Role of Nuclear Chromosomes in Development.” Developmental Biology 314: 1–11.CrossRefGoogle Scholar
  62. Laubichler, Manfred, and Maienschein, Jane. 2007. From Embryology to Evo-Devo: A History of Developmental Evolution. Cambridge, MA: MIT Press.Google Scholar
  63. Laubichler, Manfred, and Rheinberger, Hans-Jörg. 2004. “Alfred Kühn (1885–1968) and Developmental Evolution.” Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 302B: 103–110.CrossRefGoogle Scholar
  64. Lindee, M. Susan. 2005. Moments of Truth in Genetic Medicine. Baltimore, MD: Johns Hopkins University Press.Google Scholar
  65. Lorch, J., and Danielli, J.F. 1950. “Transplantation of Nuclei from Cell to Cell.” Nature 166: 329–330.CrossRefGoogle Scholar
  66. Löwy, Ilana. 1994. “Experimental Systems and Clinical Practices: Tumor Immunology and Cancer Immunotherapy, 1895–1980.” Journal of the History of Biology 27: 403–435.CrossRefGoogle Scholar
  67. Maienschein, Jane. 1978. “Cell Lineage, Ancestral Reminiscence, and the Biogenetic Law.” Journal of the History of Biology 11: 129–158.CrossRefGoogle Scholar
  68. Maienschein, Jane. 1991. Transforming Traditions in American Biology, 1880–1915. Baltimore: The Johns Hopkins University Press.Google Scholar
  69. Maienschein, Jane. 2001. “On Cloning: Advocating History of Biology in the Public Interest.” Journal of the History of Biology 34(3): 423–432.CrossRefGoogle Scholar
  70. Maienschein, Jane. 2005. Whose View of Life: Embryos, Cloning, and Stem Cells. Cambridge: Harvard University Press.Google Scholar
  71. McKinnell, Robert Gilmore. 1979. Cloning: A Biologist Reports. Minneapolis:University of Minnesota Press.Google Scholar
  72. McKinnell, Robert Gilmore, and Di Berardino, Marie. 1999. “The Biology of Cloning: History and Rationale.” Bioscience 49: 875–885.CrossRefGoogle Scholar
  73. Moritz, Alan R. 1971. “Howard T. Karsner, 1879–1970.” American Journal of Pathology 62: 3–5.Google Scholar
  74. Mukherjee, Siddhartha. 2010. The Emperor of All Maladies: A Biography of Cancer. New York: Scribner.Google Scholar
  75. Needham, Joseph. 1933. “On the Dissociability of the Fundamental Processes in Ontogenesis.” Biological Reviews 8: 180.CrossRefGoogle Scholar
  76. Needham, Joseph. 1936. Order and Life. New Haven, CT: Yale University Press.Google Scholar
  77. Oppenheimer, Jane M. 1966. “The Growth and Development of Developmental Biology.” M. Locke (ed.), Major Problems in Developmental Biology. New York: Academic Press, pp. 1–27.Google Scholar
  78. Patterson, Elizabeth K. 1984. “Growth – The Early History of a Cancer Research Institute: 1927–1957.” TMs (photocopy), Fox Chase Cancer Center Archives, Fox Chase, PA.Google Scholar
  79. Patterson, James T. 1987. The Dread Disease: Cancer and Modern American Culture. Cambridge, MA: Harvard University Press.Google Scholar
  80. Pauly, Philip J. 1987. Controlling Life: Jacques Loeb & the Engineering Ideal in Biology. Oxford: Oxford University Press.Google Scholar
  81. Pauly, Philip J. 1988. “Summer Resort and Scientific Discipline: Woods Hole and the Structure of American Biology, 1882–1925.” Ronald Rainger, Keith R. Benson, and Jane Maienschein (eds.), The American Development of Biology. Philadelphia: University of Pennsylvania Press, pp. 121–150.Google Scholar
  82. Pauly, Philip J. 2002. Biologists and the Promise of American Life: From Meriwether Lewis to Alfred Kinsey. Princeton: Princeton University Press.Google Scholar
  83. Pinell, Patrice. 2000. “Cancer.” Roger Cooter and David Pickstone (eds.), Medicine in the Twentieth Century. Amsterdam: Harwood Academic Publishers, pp. 671–685.Google Scholar
  84. Proctor, Robert N. 1995. Cancer Wars: How Politics Shapes What We Know and Don’t Know About Cancer. New York: Basic Books.Google Scholar
  85. Rader, Karen A. 2004. Making Mice: Standardizing Animals for American Biomedical Research, 1900–1955. Princeton: Princeton University Press.Google Scholar
  86. Rainger, Ronald, Benson, Keith, and Maienschein, Jane (eds.). 1988. The American Development of Biology. Philadelphia: University of Pennsylvania Press.Google Scholar
  87. Rather, L.J. 1978. The Genesis of Cancer: A Study in the History of Ideas. Baltimore: Johns Hopkins University Press.Google Scholar
  88. Reimann, Stanley, 1950. “[Untitled],” Growth 14.Google Scholar
  89. “Research Feat Reported: 2 Doctors Shift Living Nuclei of Nerve Cell to Another.” 1953. New York Times 31.Google Scholar
  90. Richmond, Marsha. 2007. “The Cell as the Basis for Heredity, Development, and Evolution: Richard Goldschmidt’s Program of Physiological Genetics.” Manfred Laubichler and Jane Maienschein (eds.), From Embryology to Evo-Devo: A History of Developmental Evolution. Cambridge: MIT Press.Google Scholar
  91. Rudkin, George. 1971. “Obituaries: Jack Schultz, 7 May 1904–29 April 1971.” Genetics Supplement 97–98.Google Scholar
  92. Sapp, Jan. 1983. “The Struggle for Authority in the Field of Heredity, 1900–1932: New Perspectives on the Rise of Genetics.” Journal of the History of Biology 16: 311–342.CrossRefGoogle Scholar
  93. Sapp, Jan. 1987. Beyond the Gene: Cytoplasmic Inheritance and the Struggle for Authority in Genetics. New York: Oxford University Press.Google Scholar
  94. Schultz, J. 1929. “The Minute Reaction in the Development of Drosophila melanogaster.” Genetics 14: 366–419.Google Scholar
  95. Schultz, J. 1941. “The Evidence of the Nucleoprotein Nature of the Gene.” Cold Spring Harbor Symposia on Quantitative Biology 9: 55–65.CrossRefGoogle Scholar
  96. Schultz, J. 1950. “The Question of Plasmagenes.” Science 111: 407.CrossRefGoogle Scholar
  97. Shimkin, Michael B. 1957. “Thirteen Questions: Some Historical Outlines for Cancer Research.” Journal of the National Cancer Institute 19(1957): 295–305.Google Scholar
  98. Shimkin, Michael B. 1977. Contrary to Nature. U. S. Department of Health, Education, and Welfare Publication No. (NIH) 76-720. Washington, DC: U. S. Government Printing Office.Google Scholar
  99. Sonneborn, Tracy M. 1948. “Genes, Cytoplasm, and Environment in Paramecium.” The Scientific Monthly 67(September): 154.Google Scholar
  100. Spemann, Hans. 1938. Embryonic Development and Induction. New Haven: Yale University Press.Google Scholar
  101. Stein, W. 1986. “James Frederic Danielli. 13 November 1911–22 April 1984.” Biographical Memoirs of Fellows of the Royal Society 32(December): 116–135.CrossRefGoogle Scholar
  102. Tomes, Nancy. 1998. The Gospel of Germs: Men, Women, and the Microbe in American Life. Cambridge, MA: Harvard University Press.Google Scholar
  103. Waldby, Catherine, and Mitchell, Robert. 2006. Tissue Economies: Blood, Organs, and Cell Lines in Late Capitalism. Durham: Duke University Press.CrossRefGoogle Scholar
  104. Weinberg, Robert A. 1996. Racing to the Beginning of the Road: The Search for the Origin of Cancer. New York: W. H. Freeman and Company.Google Scholar
  105. White, Philip R. 1950. “The Origins of the Journal Growth.” Growth 14: 258–261.Google Scholar
  106. Wilmut, Ian, Campbell, Keith, and Tudge, Colin. 2000. The Second Creation: Dolly and the Age of Biological Control. New York: Farrar, Straus and Giroux.Google Scholar
  107. Wilmut, Ian, Campbell, Keith, and Tudge, Colin. 2007. “The 10th Anniversary of the Dolly Experiment.” Cloning and Stem Cells 9: 1–2.CrossRefGoogle Scholar

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© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Life Science C Wing, Center for Biology and SocietyArizona State UniversityTempeUSA

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