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Expression and localization of amelotin, laminin γ2 and odontogenesis-associated phosphoprotein (ODAPH) on the basal lamina and junctional epithelium

Abstract

At maturation stage of enamel development, a specialized basal lamina (sBL) was built between ameloblasts and enamel. After the teeth eruption, the ameloblasts transform into the inner cell layer of junctional epithelium. The inner cell layer forms the internal basal lamina of junctional epithelium. However, the composition of the sBL and internal basal lamina was not clarified. The objective of our study was to make a description of the localization of amelotin (AMTN), laminin γ2 (LAMC2) and Odontogenesis-associated phosphoprotein (ODAPH) on the sBL and internal basal lamina. In immunohistochemical study, AMTN, LAMC2 and ODAPH were detected on the sBL at maturation stage. AMTN was also detected in ameloblasts at maturation stage. The expression of AMTN decreased from early-to-late maturation stage. In contrast, the expression of LAMC2 and ODAPH was stable. Immunofluorescence double-staining showed the localization of AMTN was close to enamel surface. However, the localization of ODAPH was close to ameloblasts. LAMC2 and ODAPH were observed on internal basal lamina of junctional epithelium. In contrast, no expression of AMTN was detected on internal basal lamina of junctional epithelium. Our results suggested that ODAPH might participate in enamel maturation and periodontal health, which might provide a better understanding of enamel defects and periodontal disease in clinic.

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Acknowledgements

The support by Grants from the National Natural Science Foundation of China (NSFC) (81670954) and the National Natural Science Foundation of China (NSFC) (81870738) and Shandong medical and health technology development program (2018WS533) are gratefully acknowledged.

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Correspondence to Yuguang Gao or Li Zhang.

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The authors declare that they have no competing interests.

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All experiments were performed according to the guideline of Animal Welfare Committee of Yantai Affiliated Hospital of Binzhou Medical University.

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Li, C., Gao, Y., Xu, Z. et al. Expression and localization of amelotin, laminin γ2 and odontogenesis-associated phosphoprotein (ODAPH) on the basal lamina and junctional epithelium. J Mol Histol (2021). https://doi.org/10.1007/s10735-021-10026-w

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Keywords

  • Basal lamina
  • Junctional epithelium
  • Immunohistochemistry
  • AMTN
  • LAMC2
  • ODAPH