miR-4319 inhibited retinoblastoma cells proliferation, migration, invasion and EMT progress via suppressing CD147 mediated MMPs expression

Abstract

Tumor migration is the critical step that lead to the migration in retinoblastoma (RB), in which microRNAs (miRNAs) play important roles. This study aimed to investigate the role of microRNA-4319 (miR-4319) in the development of retinoblastoma by identifying its targets, as well as its underlying regulatory mechanisms. Our data shown that miR-4319 was downregulated in RB tissues and RB cell lines. Enhanced miR-4319 suppressed cell proliferation, migration, invasion and EMT progress, promoted cell apoptosis in SO-RB50 and RB-Y79 cells. Of note, extracellular matrix metalloproteinase inducer (EMMPRI/CD147) was identified as a direct target gene for miR-4319. MMPs were regulated by CD147 and participated in the miR-4319 regulatory network in SO-RB50 cells. In addition, overexpression of CD147 abrogated the inhibitory effect of miR-4319 on RB cells. In summary, miR-4319 overexpression suppressed cell proliferation, migration and invasion may through suppressing the CD147 mediated MMPs expression, suggesting that miR-4319 may serve as a potential diagnostic biomarker and treatment target for RB.

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Abbreviations

RB:

Retinoblastoma

miR-4319:

MicroRNA-4319

EMT:

Epithelial-mesenchymal transition

MMPs:

Matrix metalloproteinases

ECM:

Extracellular matrix

EMMPRIN/CD147:

Extracellular matrix metalloproteinase inducer

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Correspondence to Yanfang Liu.

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This study was approved by the Xi'an Children’s Hospital ethics committee, and the guardians of all subjects provided informed consent for participation.

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Wu, Z., Chen, L., Zhang, C. et al. miR-4319 inhibited retinoblastoma cells proliferation, migration, invasion and EMT progress via suppressing CD147 mediated MMPs expression. J Mol Histol (2021). https://doi.org/10.1007/s10735-020-09946-w

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Keywords

  • Retinoblastoma
  • miRNA-4319
  • CD417
  • MMPs
  • EMT