Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma

Abstract

Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress, through challenge with conditionally tumorigenic cell line BHK-21/C13. 20 male NMRI mice were randomly divided into 2 groups—control and experimental. Each mouse was subcutaneously inoculated with BHK-21/C13 cells. Stress in the experimental group was induced for 20 days. After the experiment, tumor masses were removed, and analyzed using histology and immunohistochemistry techniques. We found a statistically significant difference (p = 0.034) in tumor expression and tumor volumes (p = 0.0061) between groups, as well as in immunopositivity on Ki67, cytochrome C and matrix metalloproteinase 9. Absence of immune infiltrate was noticed in experimental, and the presence of inflammatory infiltrate at tumor invasion front in control group.

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Correspondence to Pavle Banović.

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Smieško, G., Banović, P., Gusman, V. et al. Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma. J Mol Hist 51, 367–374 (2020). https://doi.org/10.1007/s10735-020-09886-5

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Keywords

  • Cytochrome C
  • Ki67
  • Fibrosarcoma
  • MMP9
  • Restrain stress