Journal of Molecular Histology

, Volume 50, Issue 4, pp 369–374 | Cite as

Akt plays indispensable roles during the first cell lineage differentiation of mouse

  • Songhua Xu
  • Lili Pang
  • Yue Liu
  • Xiuli Lian
  • Kaien Mo
  • Ruimin Lv
  • Huimin Zhu
  • Chengyu Lv
  • Jianmin Lin
  • Jiandong Sun
  • Lixuan Xu
  • Shie WangEmail author
Original Paper


The first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of the morula. Akt-specific inhibitor MK2206 significantly inhibited mouse blastocyst formation and resulted in decreased expression of the trophectoderm marker Cdx2 and led to granular distribution of ERα in the cytoplasm. Furthermore, knockdown of ERα by siRNA microinjection can also lead to a decrease in the development rate of mouse blastocysts, accompanied by a decrease in the expression level of Yap protein. We conclude that Akt may be indispensable for the first cell lineage differentiation of mouse.


Akt Blastocyst formation Yap Immunofluorescence Mouse 



This work was supported by grants from National Natural Science Foundation of China (81671526), Fujian Provincial Science and Technology Innovation Joint Fund Project (2017Y9114), Fujian Provincial Project of Education and Science for Young and Middle-aged Teachers (JAT170212), Fujian Provincial Health and Family Planning Research Talents Training Project (2017-1-67) and Science Fund for Distinguished Young Scholars of Fujian Province University (2018B028).

Compliance with ethical standards

Conflict of interest

None of the authors have any conflict of interest to declare.

Supplementary material

10735_2019_9833_MOESM1_ESM.docx (13.6 mb)
Supplementary material 1 (DOCX 13952 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province UniversityFuzhouPeople’s Republic of China
  2. 2.Department of Human Anatomy, Histology and Embryology, School of Basic Medical SciencesFujian Medical UniversityFuzhouPeople’s Republic of China
  3. 3.Fujian Key Laboratory of Medical Bioinformatics, School of Basic Medical SciencesFujian Medical UniversityFuzhouPeople’s Republic of China
  4. 4.Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical SciencesFujian Medical UniversityFuzhouPeople’s Republic of China

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