VEGFA and VEGFR2 RNAscope determination in gastric cancer
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Gastric cancer is the fifth most common cancer and third leading cause of cancer-related death worldwide. Several studies on angiogenic blocking agents in gastric cancer revealing promising results by the use of monoclonal antibodies against VEGFA or its receptor VEGFR2 or against VEGFA activating pathway. The validation of biomarkers useful to better organize the clinical trials involving anti-angiogenic therapies is crucial. Molecular markers such as RNA are increasingly used for cancer diagnosis, prognosis, and therapy guidance as in the case of the targeted therapies concerning the inhibition of angiogenesis. The aim of this study is to set the conditions for evaluating the expression of VEGFA and VEGFR2 in gastric cancer specimens and in healthy gastric mucosa by the use of RNAscope, a novel RNA in situ hybridization (ISH) method that allows the visualization of a specific gene expression in individual cells. We found the increased expression of VEGFA in the tubular glands and VEGFR2 in the endothelium of gastric cancer samples mainly in the T2, T3 and T4 stages of tumor progression as compared to the healthy controls. These results obtained by the application of this highly sensitive method for oligonucleotide detection the role of angiogenesis in gastric cancer progression already highlighted by conventional immunohistochemical methods, and offer significant promise as a new platform for developing and implementing RNA-based molecular diagnostics also in the conditions in which immunohistochemistry is not applicable.
KeywordsGastric cancer Immunohistochemistry RNAscope VEGFA VEGFR2
This work was supported by Grant from Associazione “Il Sorriso di Antonio” (Corato, Italy) to D.R. The Authors thanks Mrs. M. De Giorgis for her skillful technical assistance.
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Conflict of interest
All authors have seen and approved the manuscript being submitted and have no conflict of interest to declare.
- Caporarello N, Lupo G, Olivieri M, Cristaldi M, Cambria MT, Salmeri M, Anfuso CD (2017) Classical VEGF, Notch and Ang signalling in cancer angiogenesis, alternative approaches and future directions (Review). Mol Med Rep 16:4393–4402. https://doi.org/10.3892/mmr.2017.7179 CrossRefPubMedPubMedCentralGoogle Scholar
- Di Costanzo F, Bozzetti F, Silvestrini R (2011) Basi scientifiche per la definizione di linee-guida in ambito clinico per il Carcinoma Gastrico Istituto Superiore di SanitàGoogle Scholar
- Global Burden of Disease Cancer C et al. (2017) Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: a systematic analysis for the global burden of disease study JAMA Oncol 3:524–548 https://doi.org/10.1001/jamaoncol.2016.5688 CrossRefGoogle Scholar
- Nienhuser H, Schmidt T (2017) Angiogenesis and anti-angiogenic therapy in gastric cancer Int J Mol Sci 19 https://doi.org/10.3390/ijms19010043
- Scott LJ (2018) Apatinib: a review in advanced gastric cancer and other advanced cancers drugs https://doi.org/10.1007/s40265-018-0903-9