Journal of Molecular Histology

, Volume 49, Issue 3, pp 277–287 | Cite as

Effects of CD100 promote wound healing in diabetic mice

  • Fang Wang
  • Bei Liu
  • Zhou Yu
  • Tong Wang
  • Yajuan Song
  • Ran Zhuang
  • Yonghong Wu
  • Yingjun Su
  • Shuzhong Guo
Original Paper


Diabetes is a condition that causes delayed wound healing and results in chronic wounds. CD100 has been reported to promote and induce potent and obvious angiogenesis both in vivo and in vitro studies, the absence of which are a main cause of the diabetic chronic wound. In the present study, we investigated the effects of application of soluble CD100 on wound healing in diabetic mice. Four 5-mm full-thickness dermal wounds were made on each male db/db mouse. 12 mice from CD100 group were subcutaneously injected with 250 ng of CD100 (50 µl) per wound, in addition, 12 mice were injected with the same volume phosphate-balanced solution as the control. The animals were treated every other day until the wounds healed completely. Images were obtained to calculate the area ratio of the original area. HE and Masson’s trichrome staining were used for histological examination. Collagen remodeling, angiogenesis and wound bed inflammation were evaluated by immunohistochemical staining and western blot. We demonstrated that CD100 had distinct functions during the wound healing process. Histological and western blotting analysis showed a more organized epithelium and dermis, more collagen fibers, higher angiogenesis and lower inflammation in the CD100 group than in the PBS group. These findings suggest that CD100 may accelerate wound healing in diabetic mice by promoting angiogenesis in the wound and by reducing the inflammatory response.


Wound healing CD100 Diabetes Angiogenesis Inflammation 



Basic fibroblast growth factor


Cluster of differentiation 100


Cluster of differentiation 31


Cluster of differentiation 34


Cluster of differentiation 68


Cluster of differentiation 72

Col I

Collagen type I


Collagen type III




Human growth factor




Phosphate-balanced solution


Tumor necrosis factor alpha



This work was supported by the National Natural Science Foundation of China (81401598). The authors would like to thank Prof. Yi Chenggang for helpful discussions of the data and Dr. Chenlin and Liang Yingzi for assistance with the morphological evaluation.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest to disclose.

Supplementary material

10735_2018_9767_MOESM1_ESM.jpg (12 kb)
Supplementary Figure 1 Body weights of db/db mice and db/m mice. (JPG 12 KB)
10735_2018_9767_MOESM2_ESM.jpg (14 kb)
Supplementary Figure 2 Blood glucoseof db/db mice and db/m mice. (JPG 13 KB)
10735_2018_9767_MOESM3_ESM.jpg (355 kb)
Supplementary Figure 3 VEGF staining analysis. IHC results showing the VEGF-stained wound tissues from days 7, 13 and 21 in the PBS- and CD100-treated groups. Magnification 400×. Bars=100 μm. (JPG 355 KB)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Fang Wang
    • 1
    • 2
  • Bei Liu
    • 1
    • 3
  • Zhou Yu
    • 1
  • Tong Wang
    • 1
  • Yajuan Song
    • 1
  • Ran Zhuang
    • 4
  • Yonghong Wu
    • 3
  • Yingjun Su
    • 1
  • Shuzhong Guo
    • 1
  1. 1.Department of Plastic and Reconstructive Surgery, Xijing HospitalThe Fourth Military Medical UniversityXi’anChina
  2. 2.Department of Medical CosmetologyThe First Affiliated Hospital of Xian Medical UniversityXi’anChina
  3. 3.Department of Medical TechnologyXian Medical UniversityXi’anChina
  4. 4.Department of Transplantation Immunology Laboratory of Basic Medical CollegeThe Fourth Military Medical UniversityXi’anChina

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