Determining protein biomarkers for DLBCL using FFPE tissues from HIV negative and HIV positive patients

Magangane, Pumza; Sookhayi, Raveendra; Govender, Dhirendra; Naidoo, Richard

doi:10.1007/s10735-016-9695-y

Original Paper
Published: 01 October 2016

Determining protein biomarkers for DLBCL using FFPE tissues from HIV negative and HIV positive patients

Pumza Magangane¹,
Raveendra Sookhayi¹,
Dhirendra Govender¹ &
Richard Naidoo¹

Journal of Molecular Histology volume 47, pages 565–577 (2016)Cite this article

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Abstract

DLBCL is the most common lymphoma subtype occurring in older populations as well as in younger HIV infected patients. The current treatment options for DLBCL are effective for most patients yet the relapse rate is high. While many biomarkers for DLBCL exist, they are not in clinical use due to low sensitivity and specificity. In addition, these biomarkers have not been studied in the HIV context. Therefore, the identification of new biomarkers for HIV negative and HIV positive DLBCL, may lead to a better understanding of the disease pathology and better therapeutic design. Protein biomarkers for DLBCL were determined using MALDI imaging mass spectrometry (IMS) and characterised using LC–MS. The expression of one of the biomarkers, heat shock protein (Hsp) 70, was confirmed on a separate cohort of samples using immunohistochemistry. The biomarkers identified in the study consisted of four protein clusters including glycolytic enzymes, ribosomal proteins, histones and collagen. These proteins could differentiate between control and tumour tissue, and the DLBCL immunohistochemical subtypes in both cohorts. The majority (41/52) of samples in the confirmation cohort were negative for Hsp70 expression. The HIV positive DLBCL cases had a higher percentage of cases expressing Hsp70 than their HIV negative counterparts. The non-GC subtype also frequently overexpressed Hsp70, confirming MALDI IMS data. The expression of Hsp70 did not correlate with survival in both the HIV negative and HIV positive cohort. This study identified potential biomarkers for HIV negative and HIV positive DLBCL from FFPE tissue sections. These may be used as diagnostic and prognostic markers complementary to current clinical management programmes for DLBCL.

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Acknowledgments

The work was supported by grants from the National Research Foundation, Cancer Association of South Africa, National Health Laboratory Service Research Trust and the University of Cape Town.

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Division of Anatomical Pathology, Department of Pathology, Faculty of Health Sciences, University of Cape Town/National Health Laboratory Service, Anzio Road, Observatory, Cape Town, 7925, South Africa
Pumza Magangane, Raveendra Sookhayi, Dhirendra Govender & Richard Naidoo

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Pumza Magangane
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Raveendra Sookhayi
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Dhirendra Govender
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Richard Naidoo
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Correspondence to Richard Naidoo.

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Magangane, P., Sookhayi, R., Govender, D. et al. Determining protein biomarkers for DLBCL using FFPE tissues from HIV negative and HIV positive patients. J Mol Hist 47, 565–577 (2016). https://doi.org/10.1007/s10735-016-9695-y

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Received: 15 July 2016
Accepted: 08 September 2016
Published: 01 October 2016
Issue Date: December 2016
DOI: https://doi.org/10.1007/s10735-016-9695-y

Keywords

MALDI IMS
DLBCL
Biomarker
Proteome
Heat shock protein