Abstract
To clarify the role of CBX7 deficiency in dentin and alveolar bone development, the dental and mandibular phenotypes of homozygous CBX7-knockout (CBX7−/−) mice were compared with their wild-type (WT) counterparts at 3 weeks age. In contrast to WT littermates, dental volume and dentin sialoprotein-positive area were significantly increased, whereas the area ratio of predentin to dentin was decreased markedly in CBX7−/− mice. Mineral density, cortical thickness, alveolar bone volume, type I collagen and osterix-immunopositive area, osteoblast number and activity, protein expression and mRNA level of Runt-related transcription factor 2 (Runx2), alkaline phosphatase, osteocalcin, osteopontin and bone morphogenetic protein 2 (BMP2) were all remarkably increased, while osteoclast number and activity, and mRNA expression ratio of NF-κB ligand (RANKL) to osteoprotegerin (opg) were all decreased significantly in the alveolar bone of CBX7−/− mice compared with their WT counterparts. Moreover, proliferating cell nuclear antigen (PCNA)-positive cells were found more in Hertwig’ s epithelial root sheath of CBX7−/− mice, and their protein level of cyclin E1, cyclin-dependent kinase 2 (CDK2) were correspondingly increased in contrast to WT mice. Taken together, these results of this study suggest that CBX7 deficiency plays a positive role in dentin and alveolar bone formation.
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Acknowledgments
This study was supported in part by the National Basic Research Program of China (2012CB966902), the National Natural Science Foundation of China (81271109), and The Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, 2014-37). The authors have no conflicts of interest to disclose.
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Zhixuan Zhou and Ying Yin have contributed equally to this work.
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Zhou, Z., Yin, Y., Jiang, F. et al. CBX7 deficiency plays a positive role in dentin and alveolar bone development. J Mol Hist 47, 401–411 (2016). https://doi.org/10.1007/s10735-016-9682-3
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DOI: https://doi.org/10.1007/s10735-016-9682-3