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The effect of calcitriol on high mobility group box 1 expression in periodontal ligament cells during orthodontic tooth movement in rats

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Abstract

High mobility group box 1 (HMGB1) is a late inflammatory cytokine that plays an important role in periodontal tissue remodeling during orthodontic tooth movement. Calcitriol (1,25-dihydroxyvitamin D3 [1α,25 (OH)2D3]) is a systemic calcium-regulating hormone shown to downregulate expression of multiple proinflammatory cytokines in human periodontal ligament cells in response to orthodontic force. The purpose of this study was to investigate the effect of 1α,25(OH)2D3 on the expression of HMGB1 in periodontal ligament (PDL) cells during orthodontic tooth movement. Seven-week-old male Wistar rats were used for experimentation. Tooth movement was assessed using a nickel-titanium coil spring to apply mechanical loading to the tooth for 5 days. This was followed by administration of either 1α,25(OH)2D3 or normal saline by gavage every other day for up to 28 days. Immunohistochemistry was used to analyze the expression of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6 and HMGB1. After discontinuation of orthodontic force, expression of the early inflammatory cytokines IL-6 and TNF-α were time-dependently reduced in the 1α,25(OH)2D3 group compared with the control group at each time point. Similarly, expression of HMGB1 was decreased over time in both the 1α,25(OH)2D3 and normal saline groups, and 1α,25(OH)2D3 administration enhanced this decline. These findings indicate that administration of 1α,25(OH)2D3 might provide a favorable microenvironment for orthodontic tooth movement by downregulating expression of HMGB1 in PDL cells.

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Acknowledgments

This study was partially supported by the National Nature Science Foundation of China (Grant Nos. 81271965; 81470719; 81311140173; 8151101150) and Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20120131110073) to Li M.

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Authors and Affiliations

  1. Department of Bone Metabolism, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Wenhua West Road 44-1, Jinan, 250012, China

    Jian Cui, Juan Li, Wei Wang, Xiuchun Han, Juan Du, Jing Sun, Wei Feng, Bo Liu, Hongrui Liu & Minqi Li

  2. Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan

    Norio Amizuka

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  1. Jian Cui
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  2. Juan Li
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  3. Wei Wang
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  10. Norio Amizuka
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  11. Minqi Li
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Correspondence to Minqi Li.

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Cui, J., Li, J., Wang, W. et al. The effect of calcitriol on high mobility group box 1 expression in periodontal ligament cells during orthodontic tooth movement in rats. J Mol Hist 47, 221–228 (2016). https://doi.org/10.1007/s10735-016-9669-0

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  • DOI: https://doi.org/10.1007/s10735-016-9669-0

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