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A novel method using an acedan-based Zn(DPA) probe to monitor ATP localization in an in vivo system

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Abstract

In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it shows non-specific nuclear staining both in vitro and in vivo. A commercial luciferin-luciferase bioluminescence assay has also been used to detect ATP, but it can not be easily used in vivo. Thus, to effectively monitor ATP in vivo, we employed a novel two-photon ATP fluorescent probe, acedan-based Zn(DPA). Using the acedan-based Zn(DPA) probe, we show that this probe produces high quality images of ATP in lung, spleen, liver and spinal cord tissues.

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Acknowledgments

This work was supported by the Global Frontier Project grant (2012M3A6A4054932) of the National Research Foundation funded by the Ministry of Education, Science and Technology of Korea.

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Authors and Affiliations

  1. Department of Anatomy, College of Medicine, Kyung Hee University, Heogi-Dong 1, Dongdaemun-Gu, Seoul, 130-701, Republic of Korea

    Seo Jin Lee, Youn Ho Shin, Youngbuhm Huh & Junyang Jung

  2. Department of Chemistry, POSTECH, San 31, Hyoja-dong, Pohang, 790-784, Republic of Korea

    Alla Sreenivasa Rao & Kyo Han Ahn

  3. Department of Anesthesiology and Pain Medicine, Kosin University College of Medicine, 34 Amnam-dong, Seo-gu, Busan, 602-703, Republic of Korea

    Hyung-Joo Chung

Authors
  1. Seo Jin Lee
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  2. Alla Sreenivasa Rao
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  3. Youn Ho Shin
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  4. Hyung-Joo Chung
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  5. Youngbuhm Huh
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  6. Kyo Han Ahn
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  7. Junyang Jung
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Correspondence to Junyang Jung.

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Lee, S.J., Rao, A.S., Shin, Y.H. et al. A novel method using an acedan-based Zn(DPA) probe to monitor ATP localization in an in vivo system. J Mol Hist 44, 241–247 (2013). https://doi.org/10.1007/s10735-012-9474-3

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  • DOI: https://doi.org/10.1007/s10735-012-9474-3

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