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Differential expression and localization of dentin matrix protein 1 (DMP1) fragments in mouse submandibular glands

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Abstract

It has been demonstrated that dentin matrix protein 1 (DMP1) is an essential regulator in the formation of bone and tooth. In addition to the mineralized tissues, DMP1 is also expressed in the non-mineralized tissues such as kidney, brain and salivary glands. Some studies have shown that the expression of DMP1 is significantly elevated in cancerous glands, while details about the expression and localization patterns of DMP1 in these glandular tissues still remain largely unknown. In this study, with multiple approaches, we systematically analyzed the expression and localization of DMP1 in mouse submandibular glands (SMGs). The results showed that although DMP1 was expressed in both female and male mouse SMGs, the mRNA levels of DMP1 in male mice were higher than those in female mice after the appearance of granular convoluted tubule (GCT). In mouse SMGs, DMP1 was primarily present as the 46 kDa C-terminal fragment and the 37 kDa N-terminal fragment. The C-terminal fragment was mainly localized in the nuclei of acinar and ductal cells, while the N-terminal fragment was restricted to the cytoplasm of ductal cells. This study showed the expression of DMP1 in the GCT of male mice, a novel finding different from the result of previous reports. Collectively, the differential localization patterns of DMP1 fragments indicate that different forms of DMP1 may play distinct roles in the SMGs.

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References

  • Baba O, Qin C, Brunn JC, Wygant JN, McIntyre BW, Butler WT (2004) Colocalization of dentin matrix protein 1 and dentin sialoprotein at late stages of rat molar development. Matrix Biol 23:371–379

    Article  PubMed  CAS  Google Scholar 

  • Chaplet M, De Leval L, Waltregny D, Detry C, Fornaciari G, Bevilacqua G, Fisher LW, Castronovo V, Bellahcene A (2003) Dentin matrix protein 1 is expressed in human lung cancer. J Bone Miner Res 18:1506–1512

    Article  PubMed  CAS  Google Scholar 

  • Feng JQ, Zhang J, Dallas SL, Lu Y, Chen S, Tan X, Owen M, Harris SE, MacDouquall M (2002) Dentinmatrix protein 1, a targetmolecule for Cbfa1 in bone, is a unique bone marker gene. J Bone Miner Res 17:1822–1831

    Article  CAS  Google Scholar 

  • Feng JQ, Huang H, Lu Y, Ye L, Xie Y, Tsutsui TW, Kunieda T, Castranio T, Scott G, Bonewald LB, Mishina Y (2003) The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. J Dent Res 82:776–780

    Article  PubMed  CAS  Google Scholar 

  • Feng JQ, Ward LM, Liu S, Lu Y, Xie Y, Yuan B, Yu X, Rauch F, Davis SI, Zhang S, Rios H, Drezner MK, Quarles LD, Bonewald LF, White KE (2006) Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism. Nat Genet 38:1310–1315

    Article  PubMed  CAS  Google Scholar 

  • Fisher LW, Fedarko NS (2003) Six genes expressed in bones and teeth encode the current members of the SIBLING family of proteins. Connect Tiss Res 44:33–40

    CAS  Google Scholar 

  • Fisher LW, Torchia DA, Fohr B, Young MF, Fedarko NS (2001) Flexible structures of SIBLING proteins, bone sialoprotein, and osteopontin. Biochem Biophys Res Commun 280:460–465

    Article  PubMed  CAS  Google Scholar 

  • Fisher LW, Jain A, Tayback M, Fedarko NS (2004) Small integrin binding ligand N-linked glycoprotein gene family expression in different cancers. Clin Cancer Res 10:8501–8511

    Article  PubMed  CAS  Google Scholar 

  • Gajjeraman S, Narayanan K, Hao J, Qin C, George A (2007) Matrix macromolecules in hard tissues control the nucleation and hierarchical assembly of hydroxyapatite. J Biol Chem 282:1193–1204

    Article  PubMed  CAS  Google Scholar 

  • George A, Sabsay B, Simonian PA, Veis A (1993) Characterization of a novel dentin matrix acidic phosphoprotein: implications for induction of biomineralization. J Biol Chem 268:12624–12630

    PubMed  CAS  Google Scholar 

  • Gericke A, Qin C, Sun Y, Redfern R, Redfern D, Fujimoto Y, Taleb H, Butler WT, Boskey AL (2010) Different forms of DMP1 play distinct roles in mineralization. J Dent Res 89:355–359

    Article  PubMed  CAS  Google Scholar 

  • Gresik EW (1980) Postnatal developmental changes in submandibular glands of rats and mice. J Histochem Cytochem 28:860–870

    Article  PubMed  CAS  Google Scholar 

  • Gresik EW (1994) The granular convoluted tubule (GCT) cell of rodent submandibular glands. Microsc Res Tech 27:1–24

    Article  PubMed  CAS  Google Scholar 

  • He G, Gajjeraman S, Schultz D, Cookson D, Qin C, Butler WT, Hao J, George A (2005) Spatially and temporally controlled biomineralization is facilitated by interaction between self-assembled dentin matrix protein 1 and calcium phosphate nuclei in solution. Biochemistry 44:16140–16148

    Article  PubMed  CAS  Google Scholar 

  • Hirst KL, Simmons D, Feng J, Aplin H, Dixon MJ, MacDougall M (1997) Elucidation of the sequence and the genomic organization of the human dentin matrix acidic phosphoprotein 1 (DMP1) gene: exclusion of the locus from a causative role in the pathogenesis of dentinogenesis imperfecta type II. Genomics 42:38–45

    Article  PubMed  CAS  Google Scholar 

  • Huang B, Maciejewska I, Sun Y, Peng T, Qin DS, Lu Y, Bonewald L, Butler WT, Feng JQ, Qin C (2008) Identification of full-length dentin matrix protein 1 in dentin and bone. Calcif Tiss Int 82:401–410

    Article  CAS  Google Scholar 

  • Jayasinghe NR, Cope GH, Jacob S (1990) Morphometric studies on the development and sexual dimorphism of the submandibular gland of the mouse. J Anat 172:115–127

    PubMed  CAS  Google Scholar 

  • MacDougall M, Gu TT, Luan X, Simmons D, Chen J (1998) Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues. J Bone Miner Res 13:422–431

    Article  PubMed  CAS  Google Scholar 

  • Maciejewska I, Qin D, Huang B, Sun Y, Mues G, Svoboda K, Bonewald L, Butler WT, Feng JQ, Qin C (2009) Distinct compartmentalization of dentin matrix protein 1 fragments in mineralized tissues and cells. Cells Tiss Organs 189:186–191

    Article  CAS  Google Scholar 

  • Nagel H, Laskawi R, Wahlers A, Hemmerlein B (2004) Expression of matrix metalloproteinases MMP-2, MMP-9 and their tissue inhibitors TIMP-1, -2, and -3 in benign and malignant tumours of the salivary gland. Histopathology 44:222–231

    Article  PubMed  CAS  Google Scholar 

  • Narayanan K, Ramachandran A, Hao J, He G, Park KW, Cho W, George A (2003) Dual functional roles of dentin matrix protein 1. Implications in biomineralization and gene transcription by activation of intracellular Ca2+ store. J Biol Chem 278:17500–17508

    Article  PubMed  CAS  Google Scholar 

  • Ogbureke KU, Fisher LW (2004) Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res 83:664–670

    Article  PubMed  CAS  Google Scholar 

  • Qin C, Brunn JC, Jones J, George A, Ramachandran A, Gorski JP, Butler WT (2001) A comparative study of sialic acid-rich proteins in rat bone and dentin. Oral Sci 109:133–141

    Article  CAS  Google Scholar 

  • Qin C, Brunn JC, Cook RG, Orkiszewski RS, Malone JP, Veis A, Butler WT (2003) Evidence for the proteolytic processing of dentin matrix protein 1: identification and characterization of processed fragments and cleavage sites. J Biol Chem 278:34700–34708

    Article  PubMed  CAS  Google Scholar 

  • Qin C, Huang B, Wygant JN, Mcintyre BW, McDonald CH, Cook RG, Butler WT (2006) A chondroitin sulfate chain attached to the bone dentin matrix protein 1 NH2-terminal fragment. J Biol Chem 281:8034–8040

    Article  PubMed  CAS  Google Scholar 

  • Quarles L (2008) Endocrine functions of bone in mineral metabolism regulation. J Clin Invest 118:3820–3828

    Article  PubMed  CAS  Google Scholar 

  • Sabbadini E, Berczi I (1995) The submandibular gland: a key organ in the neuro-immuno-regulatory network? Neuroimmunomodulation 2:184–202

    Article  PubMed  CAS  Google Scholar 

  • Simmer JP, Hu Y, Richardson AS, Bartlett JD, Hu JC (2011) Why does enamel in Klk4-null mice break above the dentino–enamel junction? Cells Tiss Organs 194:211–215

    Article  CAS  Google Scholar 

  • Siyam A, Wang S, Qin C, Mues G, Stevens R, D’Souza RN, Lu Y (2012) Nuclear localization of DMP1 proteins suggests a role in intracellular signaling. Biochem Biophys Res Commun 424:641–646

    Article  PubMed  CAS  Google Scholar 

  • Tartaix PH, Doulaverakis M, George A, Fisher LW, Butler WT, Qin C, Salih E, Tan M, Fujimoto Y, Spevak L, Boskey AL (2004) In vitro effects of dentin matrix protein-1 on hydroxyapatite formation provide insights into in vivo functions. J Biol Chem 279:18115–18120

    Article  PubMed  CAS  Google Scholar 

  • Terasawa M, Shimokawa R, Terashima T, Ohya K, Takagi Y, Shimokawa H (2004) Expression of dentin matrix protein 1 (DMP1) in nonmineralized tissues. J Bone Miner Metab 22:430–438

    Article  PubMed  CAS  Google Scholar 

  • Toyosawa S, Shintani S, Fujiwara T, Ooshima T, Sato A, Ijuhin N, Komori T (2001) Dentin matrix protein 1 is predominantly expressed in chicken and rat osteocytes but not in osteoblasts. J Bone Miner Res 16:2017–2026

    Article  PubMed  CAS  Google Scholar 

  • Wang X, Hao J, Xie Y, Sun Y, Hernandez B, Yamoah AK, Prasad M, Zhu Q, Feng JQ, Qin C (2010) Expression of FAM20C in the osteogenesis and odontogenesis of mouse. J Histochem Cytochem 58:957–967

    Article  PubMed  CAS  Google Scholar 

  • Ye L, MacDougall M, Zhang S, Xie Y, Zhang J, Li Z, Lu Y, Mishina Y, Feng JQ (2004) Deletion of dentin matrix protein-1 leads to a partial failure of maturation of predentin into dentin, hypomineralization, and expanded cavities of pulp and root canal during postnatal tooth development. J Biol Chem 279:19141–19148

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by National Institutes of Health (NIH) grant DE005092 and National Natural Science Foundation of China (NSFC) grant (No. 81171744). We thank Dr. Fei Liu of Texas A&M Health Science Center College of Medicine for his valuable suggestions in this study.

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Authors and Affiliations

  1. Longjiang Scholar Laboratory, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin, 150001, Heilongjiang, China

    Changcheng Li, Xiaohua Xie, Peihong Liu & Li Chen

  2. Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M University System Health Science Center, 3302 Gaston Ave. Room 400, Dallas, TX, 75246, USA

    Changcheng Li, Xiaofang Wang, Yao Sun & Chunlin Qin

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  1. Changcheng Li
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  2. Xiaohua Xie
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  3. Xiaofang Wang
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  4. Yao Sun
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  5. Peihong Liu
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  6. Li Chen
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  7. Chunlin Qin
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Correspondence to Li Chen or Chunlin Qin.

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Li, C., Xie, X., Wang, X. et al. Differential expression and localization of dentin matrix protein 1 (DMP1) fragments in mouse submandibular glands. J Mol Hist 44, 231–239 (2013). https://doi.org/10.1007/s10735-012-9464-5

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  • DOI: https://doi.org/10.1007/s10735-012-9464-5

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