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Mapping histological levels of 8-hydroxy-2′-deoxyguanosine in female reproductive organs

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Abstract

Oxidative stress is associated with many disease states including gynecologic disease. This process can damage lipids, proteins and DNA. The present study highlights the role of oxidative stress induced DNA damage as measured by 8-hydroxy-2-deoxyguanosine in development of benign gynecological conditions (BGC). Our aim was to map the oxidative DNA damage on female reproductive organs and highlight the high amount found in a variety of benign gynecologic disorders. Seventeen biopsy specimens from female pelvic organs were divided in two groups: healthy organs tissue and BGC tissue. Healthy organs biopsy tissue included the cervix, tubes, uterus, peritoneum, and topic endometrium in secretory phase. Benign gynecological biopsy tissue included hydrosalpinges, leiomyoma, adenomyosis and tubal cysts. Immunohistochemical staining showed significantly higher levels of DNA damage between BGC and healthy organs [19.36 % (6.20; 32.51) vs. 4.61 % (0.63; 8.53); P < 0.0344]. Our results highlight the involvement of oxidative stress DNA damage in female benign pelvic disease. Hydrosalpinges, leiomyoma, and adenomyosis exhibit the highest amounts of oxidative DNA damage in the pelvic cavity.

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Acknowledgments

Luiz Fernando Pina de Carvalho was supported by a Brazilian government research grant from “Coordination for the improvement of higher-level personnel” (CAPES).

Conflict of interest

None of the authors have conflict of interest.

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Authors and Affiliations

  1. Center for Reproductive Medicine, Obstetrics and Gynecology and Women’s Health Institute, Glickman Urology and Kidney Institute, 9500 Euclid Avenue, Desk A19, Cleveland, OH, 44195, USA

    Luiz Fernando Pina de Carvalho, Rakesh Sharma & Ashok Agarwal

  2. Department of Obstetrics and Gynecology, São Paulo University, São Paulo, Brazil

    Luiz Fernando Pina de Carvalho & Mauricio Simões Abrão

  3. Pathology and Lab Medicine, Anatomic Pathology, Cleveland Clinic, Cleveland, OH, 44195, USA

    Charles Biscotti

  4. Glickman Urology and Kidney Institute, Cleveland Clinic, Cleveland, OH, 44195, USA

    Rakesh Sharma & Ashok Agarwal

  5. Gynecology and Women’s Health Institute, 9500 Euclid Avenue, Desk A81, Cleveland, OH, 44195, USA

    Tommaso Falcone

Authors
  1. Luiz Fernando Pina de Carvalho
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  2. Mauricio Simões Abrão
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  3. Charles Biscotti
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  4. Rakesh Sharma
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  5. Ashok Agarwal
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  6. Tommaso Falcone
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Corresponding author

Correspondence to Rakesh Sharma.

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de Carvalho, L.F.P., Abrão, M.S., Biscotti, C. et al. Mapping histological levels of 8-hydroxy-2′-deoxyguanosine in female reproductive organs. J Mol Hist 44, 111–116 (2013). https://doi.org/10.1007/s10735-012-9454-7

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  • DOI: https://doi.org/10.1007/s10735-012-9454-7

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