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Spatiotemporal patterns and essential role of TNF receptor-associated factor 5 expression after rat spinal cord Injury

Journal of Molecular Histology volume 43, pages 527–533 (2012)Cite this article

Abstract

TRAF5 (TNF receptor-associated factor 5), which has a tumor necrosis factor receptor-associated factor (TRAF) domain in its carboxyl terminus, was identified CD40-associated factor. It was a signal transducer for NF-κB signal pathway and other pathway. To elucidate the expression and roles of TRAF5 in nervous system lesion and repair, we performed an acute spinal cord injury (SCI) model in adult rats and studied the dynamic changes of TRAF5 expression in spinal cord. Western blot and immunohistochemistry analysis revealed that TRAF5 was present in normal spinal cord. It gradually increased, reached a peak at 5 days after SCI, and then declined during the following days. Immunofluorescence double-labeling revealed that TRAF5 was co-expressed with NeuN and GFAP, respectively. Interesting, after injury, TRAF5 expression was increased predominantly in astrocytes, which highly expressed PCNA, a marker for proliferating cells. In conclusion, this is the first description of TRAF5 expression in spinal cord. Our data suggested that TRAF5 might play important roles in CNS pathophysiology after SCI.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81171140, No. 30300099, No. 30770488 and No. 30870320); Natural Science Foundation of Jiangsu province (No. BK2009161, No. BK2009156, and No. BK2009157); Natural science Foundation of Jiangsu Colleges and Universities Grant (09KJD310005); The Society and Technology Grew Project of Nantong City (S2008020).

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Affiliations

  1. Orthopedic Department, Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Weipeng Huan, Xiujie Wu, Shuangwei Zhang, Yin Zhao, Hua Xu, Na Wang, Honghui Li, Hailei Chen, Haixiang Wei & Youhua Wang

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  1. Weipeng Huan
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  2. Xiujie Wu
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  3. Shuangwei Zhang
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  4. Yin Zhao
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  5. Hua Xu
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  6. Na Wang
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  7. Honghui Li
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  8. Hailei Chen
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  9. Haixiang Wei
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  10. Youhua Wang
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Correspondence to Youhua Wang.

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Weipeng Huan and Xiujie Wu contributed equally to this work.

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Huan, W., Wu, X., Zhang, S. et al. Spatiotemporal patterns and essential role of TNF receptor-associated factor 5 expression after rat spinal cord Injury. J Mol Hist 43, 527–533 (2012). https://doi.org/10.1007/s10735-012-9411-5

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  • DOI: https://doi.org/10.1007/s10735-012-9411-5

Keywords

  • Spinal cord injury
  • TRAF5
  • Astrocytes proliferation
  • Rats