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Involvement of CtBP2 in LPS-induced microglial activation

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Abstract

CtBP2 (C-terminal binding protein 2), which is widely expressed during developmental processes and differentiation, acts as a transcriptional repressor following recruitment to target promoters through repressors or other co-repressor proteins. In this study, we elucidated the dynamic expression changes and localization of CtBP2 in lipopolysaccharide (LPS)-induced neuroinflammatory processes in adult rats. CtBP2 expression was strongly induced in active glia cells (microglia and astrocytes) in inflamed spinal cord. In vitro studies indicated that the up-regulation of CtBP2 may be involved in the subsequent microglia activation following LPS exposure. And the knock-down of CtBP2 in microglia cell line HAPI by siRNA showed that CtBP2 increased the activation of microglia induced by LPS. Collectively, these results suggested CtBP2 may be important in host defense in microglia-mediated immune response. Understanding the cell signal pathway may provide a novel strategy against inflammatory and immune reaction in neuroinflammation in central nervous system.

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Abbreviations

CtBP2:

C-terminal binding protein 2

LPS:

Lipopolysaccharide

CNS:

Central nervous system

FCS:

Fetal calf serum

TNF-α:

Tumor necrosis factor-α

BCL-3:

B-cell lymphoma 3

PAGE:

Polyacrylamide gel electrophoresis

ECL:

Enhanced chemiluminescence system

RT-PCR:

Reverse transcriptase PCR

GFAP:

Glial fibrillary acidic protein

NeuN:

Neuronal nuclei

PCNA:

Proliferating cell nuclear antigen

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30870320, No. 81070992 and No. 81172879); A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Immunology, Medical College, Nantong University, 19 Qixiu Road, Nantong, 226001, People’s Republic of China

    Guowei Zhang, Lihua Kang, Qinglei Hang, Chunmiao Li, Lin Zhu, Qin Yuan, Qiyun Wu & Chun Cheng

  2. Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, People’s Republic of China

    Yaohua Yan, Kaifu Ke, Xinmin Wu & Yilu Gao

  3. Department of Spine Surgery, The Second Affiliated Hospital of Nanhua University, Hunan, 421001, People’s Republic of China

    Qi Cao

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  1. Guowei Zhang
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  2. Yaohua Yan
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  3. Lihua Kang
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Corresponding authors

Correspondence to Qiyun Wu or Chun Cheng.

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Guowei Zhang and Yaohua Yan contributed equally to this work.

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Zhang, G., Yan, Y., Kang, L. et al. Involvement of CtBP2 in LPS-induced microglial activation. J Mol Hist 43, 327–334 (2012). https://doi.org/10.1007/s10735-012-9399-x

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