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Anti-inflammatory and antioxidant effects of infliximab on acute lung injury in a rat model of intestinal ischemia/reperfusion

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Abstract

The purpose of this study was to investigate the role of infliximab on acute lung injury induced by intestinal ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: sham, I/R and I/R+ infliximab; each group contain 10 animals. Sham group animals underwent laparotomy without I/R injury. After I/R groups animals underwent laparotomy, 1 h of superior mesenteric artery ligation were followed by 1 h of reperfusion. In the infliximab group, 3 days before I/R, infliximab (3 mg/kg) was administered by intravenously. All animals were sacrificed at the end of reperfusion and lung tissues samples were obtained for biochemical and histopathological investigation in all groups. To date, no more biochemical and histopathological changes on intestinal I/R injury in rats by infliximab treatment have been reported. Infliximab treatment significantly decreased the elevated tissue malondialdehyde levels and increased of reduced superoxide dismutase, and glutathione peroxidase enzyme activities in lung tissues samples. Intestinal I/R caused severe histopathological injury including edema, hemorrhage, increased thickness of the alveolar wall and a great number of inflammatory cells that infiltrated the interstitium and alveoli. Infliximab treatment significantly attenuated the severity of intestinal I/R injury. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase and arise in the expression of surfactant protein D in lung tissue of acute lung injury induced by intestinal I/R with infliximab therapy. It was concluded that infliximab treatment might be beneficial in acute lung injury, therefore, shows potential for clinical use. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects in acute lung injury induced by intestinal I/R.

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Authors and Affiliations

  1. Department of Pediatrics, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Ahmet Guzel

  2. Department of Histology and Embryology, Faculty of Medicine, Trakya University, Edirne, 22030, Turkey

    Mehmet Kanter & Mustafa Erboga

  3. Department of Chest Disease, Samsun Chest Disease and Chest Surgery Hospital, Samsun, Turkey

    Aygul Guzel

  4. Department of Surgery, Faculty of Medicine, Rize University, Rize, Turkey

    Ahmet Pergel

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  1. Ahmet Guzel
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  2. Mehmet Kanter
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  5. Mustafa Erboga
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Correspondence to Mehmet Kanter.

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Guzel, A., Kanter, M., Guzel, A. et al. Anti-inflammatory and antioxidant effects of infliximab on acute lung injury in a rat model of intestinal ischemia/reperfusion. J Mol Hist 43, 361–369 (2012). https://doi.org/10.1007/s10735-012-9396-0

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  • DOI: https://doi.org/10.1007/s10735-012-9396-0

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