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The effect of Rho kinase inhibitor Y-27632 on endotoxemia-induced intestinal apoptosis in infant rats

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Abstract

The aim of this study was to evaluate the effect of Rho kinase inhibitor, Y-27632 on the intestinal apoptosis in endotoxemic infant rats. Wistar albino 15–17-day-old rat pups (n = 21) were randomized to three experimental groups: (1) controls; (2) endotoxemia (LPS); and (3) endotoxemia treated with Y-27632 (LPS + Y-27632). Endotoxemia was induced in rats by intraperitoneal (i.p) injection of lipopolysaccharide (Escherichia coli serotype 0111:B4; 10 mg/kg). Y-27632 was administered 5 mg/kg i.p at three times, just, 8 and 16 h after LPS injection. Twenty-four hours after LPS injection, intestinal apoptosis was assessed by hematoxylin and eosin staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and immunohistochemistry for active caspase-3. Endotoxemia induced extensive apoptotic injury in the intestinal tissues. The administration of Y-27632 to endotoxemic infant rats caused a marked decrease in the number of apoptotic cells in both intestinal epithelium and lamina propria. In conclusion, the inhibition of Rho kinase with Y-27632 diminished the intestinal apoptotic damage induced by endotoxemia in infant rats.

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Authors and Affiliations

  1. Department of Pediatrics, School of Medicine, Dokuz Eylul University, Izmir, Turkey

    Durgul Ozdemir & Mustafa Kemal Dagdelen

  2. Department of Histology and Embryology, School of Medicine, Dokuz Eylul University, Izmir, Turkey

    Serap Cilaker & Kazim Tugyan

  3. Department of Pediatrics, School of Medicine, Gazi University, Ankara, Turkey

    Oksan Derinoz

  4. Department of Laboratory Animal Sciences, Health Sciences Institute, Dokuz Eylul University, Izmir, Turkey

    Ensari Guneli

Authors
  1. Durgul Ozdemir
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  2. Serap Cilaker
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  3. Kazim Tugyan
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  4. Mustafa Kemal Dagdelen
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  5. Oksan Derinoz
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  6. Ensari Guneli
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Correspondence to Durgul Ozdemir.

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Ozdemir, D., Cilaker, S., Tugyan, K. et al. The effect of Rho kinase inhibitor Y-27632 on endotoxemia-induced intestinal apoptosis in infant rats. J Mol Hist 43, 81–87 (2012). https://doi.org/10.1007/s10735-011-9379-6

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  • DOI: https://doi.org/10.1007/s10735-011-9379-6

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