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Specific subcellular targeting of PKCα and PKCε in normal and tumoral lactotroph cells by PMA-mitogenic stimulus

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Abstract

The variations of the intracellular localization of the individual protein kinase C (PKC) isoforms are related with their different biological functions. In this study, we have investigated the precise intracellular translocation of endogenous PKCα and PKCε in PMA-stimulated normal and tumoral lactotroph cells by using confocal and immunogold electron microscopy, which was correlated with the rate of cell proliferation of both pituitary cell phenotypes. The present results showed that the short phorbol ester incubation stimulated the proliferation of normal and tumoral lactotroph cells, as determined by the measurement of the BrdU-labelling index. The translocation of PKCα to plasma and nuclear membranes induced by PMA was more marked than that observed for PKCε in normal and tumoral lactotroph cells. Our results showed that PKCs translocation to the plasma and nuclear membranes varied from isozyme to isozyme emphasizing that PKCα could be related with the mitogenic stimulus exerted by phorbol ester. These data support the notion that specific PKC isozymes may exert spatially defined effects by virtue of their directed translocation to distinct intracellular sites.

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Acknowledgments

The authors wish to thank Dr. Cecilia Sampedro, Mrs Mercedes Guevara and Mrs. Elena Pereyra for her excellent technical assistance and native speaker Dr. Paul Hobson for revising the English of the manuscript. This work was supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Fondos para la Investigación Científica y Tecnológica (FONCyT) and the Secretaría de Ciencia y Tecnología de la Universidad Nacional de Córdoba (SECyT).

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  1. Centro de Microscopía Electrónica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Haya de la Torre esq. Enrique Barros, 5000, Cordoba, Argentina

    Juan Pablo Petiti, Silvina Gutiérrez, Jorge Humberto Mukdsi, Ana Lucía De Paul & Alicia Inés Torres

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  1. Juan Pablo Petiti
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  2. Silvina Gutiérrez
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  3. Jorge Humberto Mukdsi
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  4. Ana Lucía De Paul
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  5. Alicia Inés Torres
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Correspondence to Juan Pablo Petiti.

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Petiti, J.P., Gutiérrez, S., Mukdsi, J.H. et al. Specific subcellular targeting of PKCα and PKCε in normal and tumoral lactotroph cells by PMA-mitogenic stimulus. J Mol Hist 40, 417–425 (2009). https://doi.org/10.1007/s10735-010-9255-9

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  • DOI: https://doi.org/10.1007/s10735-010-9255-9

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