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In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar

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Abstract

We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to investigate the key regulator genes in odontogenesis. Ribosomal protein L21 (Rpl21) is one of differentially expressed genes in the E12.0 mandible. This study examined the precise expression pattern of Rpl21 mRNA in the mouse mandibular first molar by in situ hybridization. Rpl21 mRNA was expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0. Strong in situ signals were observed in the epithelial bud at E14.0, and in the enamel organ at E15.0. However, either no (E14.0) or only a weak (E15.0) in situ signal was found in the primary enamel knot at these gestational days. Rpl21 was strongly expressed in the inner enamel epithelium, cervical loop and dental lamina from E16.0 to E18.0. In addition, Rpl21 mRNA was also demonstrated in various developing cranio-facial organs. These results suggest that Rpl21 participates in the synthesis of various polypeptides which might be related to the initiation and the development of such tooth germ, and also in the synthesis of enamel components in the presecretory stage of the ameloblast. Rpl21 for protein synthesis might also be related to the morphogenesis of the developing cranio-facial organs.

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Acknowledgments

The authors wish to acknowledge support by a Grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (20390466 to H.S. and 21592331 to I.K.). The authors thank to Dr. K. Matsuo for his discussion.

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Authors and Affiliations

  1. Laboratory of Oral Pathology and Medicine, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Ming Xie, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Kengo Nagata, Yukiko Ookuma, Hiroaki Fujiwara & Hidetaka Sakai

  2. Department of Prosthodontics, School of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University, 639 Zhizaoju Road, 200011, Shanghai, China

    Ming Xie

  3. Section of Pediatric Dentistry, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Yukiko Ookuma

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  1. Ming Xie
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  2. Ieyoshi Kobayashi
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  3. Tamotsu Kiyoshima
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  4. Kengo Nagata
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  5. Yukiko Ookuma
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  6. Hiroaki Fujiwara
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  7. Hidetaka Sakai
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Correspondence to Hidetaka Sakai.

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Ming Xie, Ieyoshi Kobayashi and Tamotsu Kiyoshima contributed equally to this work.

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Xie, M., Kobayashi, I., Kiyoshima, T. et al. In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar. J Mol Hist 40, 361–367 (2009). https://doi.org/10.1007/s10735-009-9249-7

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  • DOI: https://doi.org/10.1007/s10735-009-9249-7

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