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Aquaporin 9 expression and its localization in normal skeletal myofiber

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Abstract

The examination was performed whether aquaporin (AQP) 9 is expressed in normal skeletal muscle at mRNA and protein levels. Gel electrophoresis of the reverse transcription-polymerase chain reaction (RT-PCR) product of total RNA samples of human normal muscles by oligonucleotide primers for human AQP9 showed a band of 221 basepairs, which corresponded to the basepair length between two primers of AQP9. The nucleotide sequence of RT-PCR product coincided with that of human AQP9. Immunoblot analysis revealed that the rabbit and sheep antibodies against the synthetic peptide of the C-terminal cytoplasmic domain of human AQP9 molecule reacted with a protein of approximately 30 kDa molecular weight in extracts of human normal skeletal muscles. Immunohistochemistry with our anti-AQP9 antibodies showed an immunoreaction at the myofiber surface of both type 1 and type 2 fibers with almost equal staining intensity in human skeletal muscles. The implication of AQP9 expression in skeletal myofibers was discussed.

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Acknowledgments

The authors would like to thank Mrs. T. Nagami for her typing of this manuscript. Thanks were also extended to Mrs. Y. Hirayama for her technical help. This work was partly supported by the Research Grant (17A-10 and 20B-13) for Nervous and Mental Disorders from the Ministry of Health, Labour and Welfare.

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Authors and Affiliations

  1. Department of Neurology, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama, 227-8501, Japan

    Masahiko Inoue, Yoshihiro Wakayama, Hiroko Kojima, Seiji Shibuya, Takahiro Jimi, Hajime Hara, Shoji Iijima & Hisatsugu Masaki

  2. Electron Microscope Laboratory, Showa University Fujigaoka Hospital, Yokohama, Japan

    Hiroaki Oniki

  3. Biochemical Laboratory, Showa University Fujigaoka Hospital, Yokohama, Japan

    Yoko Matsuzaki

Authors
  1. Masahiko Inoue
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  2. Yoshihiro Wakayama
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  3. Hiroko Kojima
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  4. Seiji Shibuya
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  5. Takahiro Jimi
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  6. Hajime Hara
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  7. Shoji Iijima
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  8. Hisatsugu Masaki
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  9. Hiroaki Oniki
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  10. Yoko Matsuzaki
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Correspondence to Yoshihiro Wakayama.

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Inoue, M., Wakayama, Y., Kojima, H. et al. Aquaporin 9 expression and its localization in normal skeletal myofiber. J Mol Hist 40, 165–170 (2009). https://doi.org/10.1007/s10735-009-9226-1

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  • DOI: https://doi.org/10.1007/s10735-009-9226-1

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