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Expression of matrix metalloproteinases-2 and -9 and RECK during alveolar bone regeneration in rat

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Abstract

MMPs are endopeptidases that play a pivotal role in ECM turnover. RECK is a single membrane-anchored MMP-regulator. Here, we evaluated the temporal and spatial expression of MMP-2, MMP-9, and RECK during alveolar bone regeneration. The maxillary central incisor of Wistar rats was extracted and the animals were killed at 1, 3, 7, 10, 14, 21, 28, and 42 days post-operatively (n = 3/period). The hemimaxillae were collected, demineralized and embedded in paraffin. Immunohistochemical analysis was performed by the immunoperoxidase technique with polyclonal antibodies. On day 1, polymorphonuclear cells in the blood clot presented mild immunolabeling for MMPs. During bone remodeling, osteoblasts facing new bone showed positive staining for gelatinases and RECK in all experimental periods. MMPs were also found in the connective tissue and endothelial cells. Our results show for the first time that inactive and/or active forms of MMP-2, MMP-9 and RECK are differentially expressed by osteogenic and connective cells during several events of alveolar bone regeneration. This may be important for the replacement of the blood clot by connective tissue, and in the formation, maturation and remodeling of new bone.

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Acknowledgments

We thank the participation of MS Luciana C. S. Oliveira and the Financial support provided by Fapesp (fellowship to Willian Fernando Zambuzzi 02/03928-0, Grant 99/10655-5, and Thematic Project grant 01/10707), CAPES (fellowship to Katiucia Batista da Silva Paiva), and CNPq Grant. n. 475721/2003-9.

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Authors and Affiliations

  1. Department of Endodontics, Bauru Dental School, University of Sao Paulo, Bauru, SP, Brazil

    Thais Accorsi-Mendonça

  2. Department of Biochemistry, Institute of Biology, State University of Campinas, Campinas, Brazil

    Katiúcia Batista da Silva Paiva & Willian Fernando Zambuzzi

  3. Department of Biological Sciences, Bauru Dental School, University of Sao Paulo, Bauru, Brazil

    Tânia Mary Cestari & Rumio Taga

  4. Department of Stomatology/Pathology, Bauru Dental School, University of Sao Paulo, Bauru, Brazil

    Vanessa Soares Lara

  5. Department of Biochemistry, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil

    Mari Cleide Sogayar

  6. Department of Cellular and Molecular Biology, Institute of Biology, Fluminense Federal University, Outeiro de São João Baptista s/n, Campus do Valonguinho, Centro, Niteroi, CEP:24020-150, RJ, Brazil

    José Mauro Granjeiro

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  1. Thais Accorsi-Mendonça
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  2. Katiúcia Batista da Silva Paiva
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  8. José Mauro Granjeiro
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Correspondence to José Mauro Granjeiro.

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Accorsi-Mendonça, T., Paiva, K.B.d.S., Zambuzzi, W.F. et al. Expression of matrix metalloproteinases-2 and -9 and RECK during alveolar bone regeneration in rat. J Mol Hist 39, 201–208 (2008). https://doi.org/10.1007/s10735-007-9152-z

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  • DOI: https://doi.org/10.1007/s10735-007-9152-z

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