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Expression of an intron-containing β-tubulin mRNA in catfish olfactory epithelium

Journal of Molecular Histology volume 38, pages 571–579 (2007)Cite this article

Abstract

β-Tubulin genes code for very similar proteins, sharing extensive identity in amino acid sequence within and across species, each of which manifests characteristic patterns of cell and tissue expression. While searching for olfactory specific mRNAs in the channel catfish (Ictalurus punctatus), we isolated a novel β-tubulin cDNA. In the putative ORF, 1298 nucleotides were 80–88% identical to cloned cDNAs from zebrafish to human for β-tubulin isotype IVb. This ORF is interrupted by an insert of 111 nucleotides located between the regions corresponding to exons 2 and 3 in other species. This insert lacks similarity to any sequence in the NCBI databases. We showed that this novel cDNA fragment hybridizes specifically to catfish olfactory epithelium mRNA on Northern analysis. Here we demonstrate by in situ analysis of catfish olfactory epithelium that the expression of this mRNA is spatially restricted to the outer two-thirds of each olfactory lamella where olfactory receptor neurons reside. These results suggest that this nucleotide sequence is the result of incomplete RNA transcript processing. The growing awareness of the regulatory roles played by RNAs transcribed from intronic regions of genes suggests that this observation may have relevance to regulation of gene expression in olfactory tissue during development and axon targeting.

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Acknowledgments

We thank R. Wurzburger for oligonucleotide synthesis and DNA sequencing, A. Porta for genomic DNA preparation, Linda Biagini and A. Puche for assistance with histology and figure preparation respectively. Supported in part by NIH-DC03112 (FLM) and the Roche Institute of Molecular Biology (EB).

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Author notes

  1. Ezio Bettini

    Present address: Screening & Compound Profiling, GlaxoSmithKline Research and Development, 37100, Verona, Italy

Affiliations

  1. Department of Anatomy and Neurobiology, School of Medicine, University of Maryland, HSF 2, 20 Penn Street, Baltimore, MD, 21210, USA

    Ezio Bettini & Frank L. Margolis

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  1. Ezio Bettini
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  2. Frank L. Margolis
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Correspondence to Frank L. Margolis.

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Bettini, E., Margolis, F.L. Expression of an intron-containing β-tubulin mRNA in catfish olfactory epithelium. J Mol Hist 38, 571–579 (2007). https://doi.org/10.1007/s10735-007-9139-9

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  • DOI: https://doi.org/10.1007/s10735-007-9139-9

Keywords

  • PCR
  • β-Tubulin
  • Ictalurus punctatus
  • In situ hybridization
  • Olfactory