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Notch1 expression and ligand interactions in progenitor cells of the mouse olfactory epithelium

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Abstract

Despite the relatively simplified organization of the olfactory epithelium (OE), our understanding of the factors that regulate its cellular diversity is limited. Genetic and localization studies suggest that Notch signaling may be important in this process. We characterize here a population of Notch1 + olfactory basal cells in embryonic mice that coordinately express both the Notch effector Hes5 and the glycosyltransferase Lfng. These cells are distinct from Mash1 + neuronal precursors, but give rise to sensory neurons, suggesting that Notch1 signals may in part function to maintain a neurogenic progenitor pool. Furthermore, Lfng + cells also generate a population of cells in the migratory mass that appear to be ensheathing glial precursors, indicating potential multipotency in these progenitors. The Notch ligand Dll4 is expressed by basal OE cells that are interspersed with Notch1 + progenitors during later OE neurogenesis. In contrast, mice deficient in Dll1 exhibit a smaller OE and a loss of Hes5 expression, indicating an earlier function in olfactory progenitor cell development. Taken together, these results further support a role for Notch signaling in the regulation of olfactory neurogenesis and cell diversity.

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Acknowledgements

We thank Andre Goffinet for providing CXCR4 cDNAs. This work was supported by National Institute of Health grants DC00953 to G.A.S. and DC06496 to T.R.H., and NS036437 to T.G.

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  1. Shriver Center/UMMS, 200 Trapelo Road, Waltham, MA, 02452, USA

    Gerald A. Schwarting & Timothy R. Henion

  2. Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, 01655, USA

    Gerald A. Schwarting & Timothy R. Henion

  3. The Jackson Laboratory, Bar Harbor, ME, 04660, USA

    Thomas Gridley

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  1. Gerald A. Schwarting
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Correspondence to Gerald A. Schwarting.

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Schwarting, G.A., Gridley, T. & Henion, T.R. Notch1 expression and ligand interactions in progenitor cells of the mouse olfactory epithelium. J Mol Hist 38, 543–553 (2007). https://doi.org/10.1007/s10735-007-9110-9

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