Abstract
Annexin A8 is a member of the annexin family of calcium-regulated membrane-binding proteins. In this report, we investigated the expression of annexin A8 in adult mouse organs. Northern blot analysis of adult mouse organs showed that a single annexin A8 transcript of 1.9 kb is expressed most strongly in skin, eye and tongue. In situ hybridisations using annexin A8-specific probes revealed that in the stratified epithelia of the tongue and the early postnatal epidermis, annexin A8 transcription could be detected in basal and suprabasal layers of these stratified epithelia. Western blot analyses using a murine ANXA8-specific antiserum showed, that the 36 kD ANXA8 protein was most abundant in the skin and tongue. The abundance of ANXA8 protein in the skin increased during postnatal days 1–18 and was immunohistochemically localised in suprabasal layers of the epidermis. In the tongue epithelium as well, ANXA8 protein was found in suprabasal layers. ANXA8 immunoreactivity was also found in suprabasal layers of the stratified epithelia of the oesophagus and the forestomach, while it was detected in all layers of the cornea epithelium and in the cornea endothelium of the eye. We also investigated the expression of retinoic acid receptor alpha protein (RARA) and ANXA8 in the epidermis immunohistochemically. While RARA immunoreactivity was exclusively detected in the basal layer, ANXA8 immunoreactivity was restricted to suprabasal layers of the epidermis. Thus, ANXA8 protein is most abundant in stratified epithelia of the postnatal mouse. Its location in the suprabasal layers suggests that ANXA8 may be associated with the terminal differentiation of epithelial cells in these tissues.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnes JA, Gomes AV (2002) Proteolytic signals in the primary structure of annexins. Mol Cell Biochem 231:1–7
Brown NL, Knott L, Halligan E, Yarram SJ, Mansell JP, Sandy JR (2003) Microarray analysis of murine palatogenesis: temporal expression of genes during normal palate development. Dev Growth Differ 45:153–165
Chang KS, Wang G, Freireich EJ, Daly M, Naylor SL, Trujillo JM, Stass SA (1992) Specific expression of the annexin VIII gene in acute promyelocytic leukemia. Blood 79:1802–1810
Fernandez MP, Copeland NG, Gilbert DJ, Jenkins NA, Morgan RO (1998) The genetic origin of mouse annexin VIII. Mamm Genome 9:8–14
Gerke V, Moss SE (2002) Annexins: from structure to function. Physiol Rev 82:331–371
Gerke V, Moss SE (1997) Annexins and membrane dynamics. Biochim Biophys Acta 1357:129–154
Goebeler V, Ruhe D, Gerke V, Rescher U (2006) Annexin A8 displays unique phospholipid and F-actin binding properties. FEBS Lett 580:2430–2434
Gottsch JD, Seitzman GD, Margulies EH, Bowers AL, Michels AJ, Saha S, Jun AS, Stark WJ, Liu SH (2003) Gene expression in donor corneal endothelium. Arch Ophthalmol 121:252–258
Hauptmann R, Maurer-Fogy I, Krystek E, Bodo G, Andree H, Reutelingsperger CP (1989) Vascular anticoagulant beta: a novel human Ca2+/phospholipid binding protein that inhibits coagulation and phospholipase A2 activity. Its molecular cloning, expression and comparison with VAC-alpha. Eur J Biochem 185:63–71
Kurosawa H, Goi K, Inukai T, Inaba T, Chang KS, Shinjyo T, Rakestraw KM, Naeve CW, Look AT (1999) Two candidate downstream target genes for E2A-HLF. Blood 93:321–332
Liu JH, Stass SA, Chang KS (1994) Expression of the annexin VIII gene in acute promyelocytic leukemia. Leuk Lymphoma 13:381–386
Liu D, Rudland PS, Sibson DR, Platt-Higgins A, Barraclough R (2000) Expression of calcium-binding protein S100A2 in breast lesions. Br J Cancer 83:1473–1479
Morgan RO, Fernandez MP (1997) Annexin gene structures and molecular evolutionary genetics. Cell Mol Life Sci 53:508–515
Moss SE, Morgan RO (2004) The annexins. Genome Biol 5:219
Oh P, Li Y, Yu J, Durr E, Krasinska KM, Carver LA, Testa JE, Schnitzer JE (2004) Subtractive proteomic mapping of the endothelial surface in lung and solid tumours for tissue-specific therapy. Nature 429:629–635
Raynal P, Pollard HB (1994) Annexins: the problem of assessing the biological role for a gene family of multifunctional calcium- and phospholipid-binding proteins. Biochim Biophys Acta 1197:63–93
Rescher U, Gerke V (2004) Annexins—unique membrane binding proteins with diverse functions. J Cell Sci 117:2631–2639
Rety S, Sopkova-de Oliveira Santos J, Dreyfuss L, Blondeau K, Hofbauerova K, Raguenes-Nicol C, Kerboeuf D, Renouard M, Russo-Marie F, Lewit-Bentley A (2005) The crystal structure of annexin A8 is similar to that of annexin A3. J Mol Biol 345:1131–1139
Reutelingsperger CP, van Heerde W, Hauptmann R, Maassen C, van Gool RG, de Leeuw P, Tiebosch A (1994) Differential tissue expression of Annexin VIII in human. FEBS Lett 349:120–124
Runkel F, Michels M, Franz T (2003) Fxyd3 and Lgi4 expression in the adult mouse: a case of endogenous antisense expression. Mamm Genome 14:665–672
Runkel F, Marquardt A, Stoeger C, Kochmann E, Simon D, Kohnke B, Korthaus D, Wattler F, Fuchs H, Hrabe de Angelis M, Stumm G, Nehls M, Wattler S, Franz T, Augustin M (2004) The dominant alopecia phenotypes Bareskin, Rex-denuded, and Reduced Coat 2 are caused by mutations in gasdermin 3. Genomics 84:824–835
Sarkar A, Yang P, Fan YH, Mu ZM, Hauptmann R, Adolf GR, Stass SA, Chang KS (1994) Regulation of the expression of annexin VIII in acute promyelocytic leukemia. Blood 84:279–286
Sohma H, Ohkawa H, Akino T, Kuroki Y (2001) Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells. J Biochem (Tokyo) 130:449–455
Stein T, Price KN, Morris JS, Heath VJ, Ferrier RK, Bell AK, Pringle MA, Villadsen R, Petersen OW, Sauter G, Bryson G, Mallon EA, Gusterson BA (2005) Annexin A8 is up-regulated during mouse mammary gland involution and predicts poor survival in breast cancer. Clin Cancer Res 11:6872–6879
Tascou S, Nayernia K, Uedelhoven J, Bohm D, Jalal R, Ahmed M, Engel W, Burfeind P (2001) Isolation and characterization of differentially expressed genes in invasive and non-invasive immortalized murine male germ cells in vitro. Int J Oncol 18:567–574
Waelput W, Verhee A, Broekaert D, Eyckerman S, Vandekerckhove J, Beattie JH, Tavernier J (2000) Identification and expression analysis of leptin-regulated immediate early response and late target genes. Biochem J 348(Pt 1):55–61
White AH, Watson RE, Newman B, Freemont AJ, Wallis GA (2002) Annexin VIII is differentially expressed by chondrocytes in the mammalian growth plate during endochondral ossification and in osteoarthritic cartilage. J Bone Miner Res 17:1851–1858
Acknowledgements
The authors are grateful to B. Blanck, G. Eversloh, U. Schwaab for excellent technical assistance, to R. Stingl for help with some RT-PCR experiments, and to T. Stein for helpful discussions.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Runkel, F., Michels, M., Franken, S. et al. Specific expression of annexin A8 in adult murine stratified epithelia. J Mol Hist 37, 353–359 (2006). https://doi.org/10.1007/s10735-006-9063-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10735-006-9063-4