Abstract
Tissue microarrays have become an essential tool in translational pathology. They are used to confirm results from other experimental platforms, such as expression microarrays, as well as a primary tool to explore the expression profile of proteins by immunohistochemical analysis. Tissue microarrays are routinely used molecular epidemiology, drug development and determining the diagnostic, prognostic and predictive value of new biomarkers. By applying traditional protein based assays, as well as novel assays to the platform, tissue microarrays have gained a new utility as a proteomic tool for both basic science as well as clinical investigation. This article will explore the new approaches that are being applied to tissue microarrays to, characterize the human proteome, and new technologies that allow tissue microarrays to function as a protein array.
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References
Braunschweig T, Chung JY, Hewitt SM (2005) Tissue microarrays: bridging the gap between research and the clinic. Expert Rev Proteom 2:325–336
Camp RL, Chung GG, Rimm DL (2002) Automated subcellular localization and quantification of protein expression in tissue microarrays. Nat Med 8:1323–1327
Chuaqui RF, Bonner RF, Best CJ, Gillespie JW, Flaig MJ, Hewitt SM, Phillips JL, Krizman DB, Tangrea MA, Ahram M, Linehan WM, Knezevic V, Emmert-Buck MR (2002) Post-analysis follow-up and validation of microarray experiments. Nat Genet 32(Suppl):509–514
Chung J-Y, Braunschweig T, Baibakov G, Galperin M, Ramesh A, Skacel M, Gannot G, Knezevic V, Hewitt SM (2006a) Transfer and multiplex immunoblotting of paraffin embedded tissue. Proteomics 6:767–774
Chung J-Y, Braunschweig T, Hu N, Roth M, Traicoff JL, Wang Q-H, Knezevic V, Taylor PR, Hewitt SM (2006b) Profiling of biomarkers in the normal to tumor transition zone of esophageal squamous cell carcinoma by multiplex tissue immunoblotting. Cancer Epidemiol Biomarkers Prev 15:1403–1408
Englert CR, Baibakov GV, Emmert-Buck MR (2000) Layered expression scanning: rapid molecular profiling of tumor samples. Cancer Res 60:1526–1530
Fejzo MS, Slamon DJ (2001) Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins. Am J Pathol 159:1645–1650
Fernandez DC, Bhargava R, Hewitt SM, Levin IW (2005) Infrared spectroscopic imaging for histopathologic recognition. Nat Biotechnol 23:469–474
Geiszt M, Lekstrom K, Brenner S, Hewitt SM, Dana R, Malech HL, Leto TL (2003) NAD(P)H oxidase 1, a product of differentiated colon epithelial cells, can partially replace glycoprotein 91phox in the regulated production of superoxide by phagocytes. J Immunol 171:299–306
Huang HE, Chin SF, Ginestier C, Bardou VJ, Adelaide J, Iyer NG, Garcia MJ, Pole JC, Callagy GM, Hewitt SM, Gullick WJ, Jacquemier J, Caldas C, Chaffanet M, Birnbaum D, Edwards PA (2004) A recurrent chromosome breakpoint in breast cancer at the NRG1/neuregulin 1/heregulin gene. Cancer Res 64:6840–6844
Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP (1998) Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 4:844–847
DeBernadi, M, Hewitt SM, Kriete A (2006) Automated confocal imaging and high-content screening for cytomics. In: Pawley JB (ed) Handbook of biological confocal microscopy, 3rd edn. Plenum Press, New York, pp 818–828
Kylaniemi M, Koskinen M, Karhunen P, Rantala I, Peltola J, Haapasalo H (2004) A novel frozen brain tissue array technique: immunohistochemical detection of neuronal paraneoplastic autoantibodies. Neuropathol Appl Neurobiol 30:39–45
Miyaji T, Hewitt SM, Liotta LA, Star RA (2002) Frozen protein arrays: a new method for arraying and detecting recombinant and native tissue proteins. Proteomics 2:1489–1493
Tangrea MA, Flaig MJ, Ramesh A, Best CJ, Baibakov GV, Hewitt SM, Mitchell CD, Hartmann DP, Knezevic V, Emmert-Buck MR, Chuaqui RF (2003) Layered expression scanning: multiplex analysis of RNA and protein gels. Biotechniques 35:1280–1285
Uhlen M, Ponten F (2005) Antibody-based proteomics for human tissue profiling. Mol Cell Proteom 4:384–393
Uhlen M, Bjorling E, Agaton C, Szigyarto CA, Amini B, Andersen E, Andersson AC, Angelidou P, Asplund A, Asplund C, Berglund L, Bergstrom K, Brumer H, Cerjan D, Ekstrom M, Elobeid A, Eriksson C, Fagerberg L, Falk R, Fall J, Forsberg M, Bjorklund MG, Gumbel K, Halimi A, Halllin I, Hamsten C, Hansson M, Hedhammar M, Hercules G, Kampf C (2005) A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol Cell Proteom 4:1920–1932
Valet G (2005) Cytomics, the human cytome project and systems biology: top-down resolution of the molecular biocomplexity of organisms by single cell analysis. Cell Prolif 38:171–174
Valet G, Leary JF, Tarnok A (2004) Cytomics – new technologies: towards a human cytome project. Cytometry A 59:167–171
Vaughan TJ, Williams AJ, Pritchard K, Osbourn JK, Pope AR, Earnshaw JC, McCafferty J, Hodits RA, Wilton J, Johnson KS (1996) Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat Biotechnol 14:309–314
Warford A (2004) Tissue microarrays: fast-tracking protein expression at the cellular level. Expert Rev Proteom 1:283–292
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This research was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.
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Chung, JY., Braunschweig, T., Tuttle, K. et al. Tissue microarrays as a platform for proteomic investigation. J Mol Hist 38, 123–128 (2007). https://doi.org/10.1007/s10735-006-9049-2
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DOI: https://doi.org/10.1007/s10735-006-9049-2