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Overexpression of caffeic acid O-methyltransferase from Lycoris aurea (LaCOMT) confers tolerance to mercury in Arabidopsis thaliana by modulating ROS homeostasis

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Abstract

Caffeic acid O-methyltransferase (COMT) catalyzes key steps in the biosynthesis of lignin. It can also act as an N-acetylserotonin O-methyltransferase (ASMT), which participates in the last step of melatonin biosynthesis. Melatonin has been demonstrated to play vital roles in the regulation of plant processes and stress responses. However, the ASMT activity of COMT has not yet been characterized in the non-model plant golden spider lily (Lycoris aurea [L’Hér.] Herb), which is an ornamental that is medicinally important. A previous transcriptome analysis identified the COMT gene (LaCOMT) in this plant. The recombinant LaCOMT protein from E. coli was highly active toward ASMT, and this activity was significantly inhibited by caffeic acid in a dose-dependent manner. LaCOMT-GFP was localized to the cytoplasm and nucleus. Considering that the bulbs of L. aurea can tolerate extreme environmental conditions, such as drought stress, waterlogging and poor soil conditions, the pattern of expression of LaCOMT in different tissues and after exposure to mercuric chloride (HgCl2) was analyzed. The results revealed that LaCOMT is ubiquitously expressed in all the tissues studied and can be induced by HgCl2. Moreover, the heterologous overexpression of LaCOMT led to mercury tolerance in transgenic Arabidopsis thaliana plants. This could be attributed to the accumulation of scavenged reactive oxygen species (ROS) by elevating antioxidant enzymes and augmenting antioxidants in the transgenic A. thaliana plants that overexpressed LaCOMT. Our results suggest that LaCOMT participates in the alleviation of Hg toxicity by modulating ROS homeostasis in plants.

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References

  • Ahammed GJ, Wu M, Wang Y, Yan Y, Mao Q, Ren J, Ma R, Liu A, Chen S (2020) Melatonin alleviates iron stress by improving iron homeostasis, antioxidant defense and secondary metabolism in cucumber. Sci Hortic 265:109205

    Article  CAS  Google Scholar 

  • Ahmad P, Alyemeni MN, Wijaya L, Ahanger MA, Ashraf M, Alam P, Paray BA, Rinklebe J (2021) Nitric oxide donor, sodium nitroprusside, mitigates mercury toxicity in different cultivars of soybean. J Hazard Mater 408:124852

    Article  CAS  PubMed  Google Scholar 

  • Arnao MB, Hernández-Ruiz J (2019) Melatonin: a new plant hormone and/or a plant master regulator? Trends Plant Sci 24:38–48

    Article  CAS  PubMed  Google Scholar 

  • Back K (2021) Melatonin metabolism, signaling and possible roles in plants. Plant J 105:376–391

    Article  CAS  PubMed  Google Scholar 

  • Battke F, Ernst D, Halbach S (2005) Ascorbate promotes emission of mercury vapour from plants. Plant Cell Environ 28:1487–1495

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Byeon Y, Lee HY, Lee K, Back K (2014) Caffeic acid O-methyltransferase is involved in the synthesis of melatonin by methylating N-acetylserotonin in Arabidopsis. J Pineal Res 57:219–227

    Article  CAS  PubMed  Google Scholar 

  • Byeon Y, Choi GH, Lee HY, Back K (2015) Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice. J Exp Bot 66:6917–6925

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cao YY, Qi CD, Li S, Wang Z, Wang X, Wang J, Ren S, Li X, Zhang N, Guo YD (2018) Melatonin alleviates copper toxicity via improving copper sequestration and ROS scavenging in cucumber. Plant Cell Physiol 60:562–574

    Article  Google Scholar 

  • Chang J, Guo Y, Yan J, Zhang Z, Yuan L, Wei C, Zhang Y, Ma J, Yang J, Zhang X, Li H (2021) The role of watermelon caffeic acid O-methyltransferase (ClCOMT1) in melatonin biosynthesis and abiotic stress tolerance. Hortic Res 8:210

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chen J, Yang ZM (2012) Mercury toxicity, molecular response and tolerance in higher plants. Biometals 25:847–857

    Article  CAS  PubMed  Google Scholar 

  • Chen Z, Chen M, Jiang M (2015) Hydrogen sulfide alleviates mercury toxicity by sequestering it in roots or regulating reactive oxygen species productions in rice seedlings. Plant Physiol Biochem 111:179–192

    Article  Google Scholar 

  • Cui W, Fang P, Zhu KK, Mao Y, Gao CY, Xie YJ, Wang J, Shen WB (2014) Hydrogen-rich water confers plant tolerance to mercury toxicity in alfalfa seedlings. Ecotox Environ Safe 105:103–111

    Article  CAS  Google Scholar 

  • Ding C, Shi G, Xu X, Yang H, Xu Y (2010) Effect of exogenous spermidine on polyamine metabolism in water hyacinth leaves under mercury stress. Plant Growth Regul 60:61–67

    Article  CAS  Google Scholar 

  • Do CT, Pollet B, Thevenin J, Sibout R, Denoue D, Barriere Y, Lapierre C, Jouanin L (2007) Both caffeoyl Coenzyme A 3-O-methyltransferase 1 and caffeic acid O-methyltransferase 1 are involved in redundant functions for lignin, flavonoids and sinapoyl malate biosynthesis in Arabidopsis. Planta 226:1117–1129

    Article  CAS  PubMed  Google Scholar 

  • Dubbels R, Reiter RJ, Klenke E, Goebel A, Schnakenberg E, Ehlers C, Schiwara HW, Schloot W (1995) Melatonin in edible plants identified by radioimmunoassay and by liquid chromatorgaphy spectrometry. J Pineal Res 18:28–31

    Article  CAS  PubMed  Google Scholar 

  • Fujita M, Hasanuzzaman M (2022) Approaches to enhancing antioxidant defense in plants. Antioxidants 11:925

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gao S, Chao OY, Tang L, Zhu JQ, Xu Y, Wang SH, Chen F (2010) Growth and antioxidant responses in Jatropha curcas seedling exposed to mercury toxicity. J Hazard Mater 182:591–597

    Article  CAS  PubMed  Google Scholar 

  • Goujon T, Sibout R, Pollet B, Maba B, Nussaume L, Bechtold N, Lu F, Ralph J, Mila I, Barrière Y, Lapierre C, Jouanin L (2003) A new Arabidopsis thaliana mutant deficient in the expression of O-methyltransferase impacts lignins and sinapoyl esters. Plant Mol Biol 51:973–989

    Article  CAS  PubMed  Google Scholar 

  • Hasan M, Ahammed GJ, Yin L, Shi K, Xia X, Zhou Y, Yu J, Zhou J (2015) Melatonin mitigates cadmium phytotoxicity through modulation of phytochelatins biosynthesis, vacuolar sequestration, and antioxidant potential in Solanum lycopersicum L. Front Plant Sci 6:601

    Article  PubMed  PubMed Central  Google Scholar 

  • Hasan MK, Ahammed GJ, Sun SC, Li MQ, Yin HQ, Zhou J (2019) Melatonin inhibits cadmium translocation and enhances plant tolerance by regulating sulfur uptake and assimilation in Solanum lycopersicum L. J Agr Food Chem 67:10563–10576

    Article  CAS  Google Scholar 

  • Hattori A, Migitaka H, Iigo M, Yamamoto K, Ohtani-Kaneko R, Hara M, Suzuki T, Reiter RJ (1995) Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebratesm. Biochem Mol Biol Int 35:627–634

    CAS  PubMed  Google Scholar 

  • Hodžić E, Balaban M, Šuškalo N, Galijašević S, Hasanagić D, Kukavica B (2019) Antioxidative response of Melissa officinalis L. and Valeriana Offcinalis L. leaves exposed to exogenous melatonin and excessive zinc and cadmium levels. J Serb Chem Soc 84:11–25

    Article  Google Scholar 

  • Hu X, Jiang M, Zhang A, Lu J (2005) Abscisic acid-induced apoplastic H2O2 accumulation up-regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves. Planta 223:57–68

    Article  CAS  PubMed  Google Scholar 

  • Iglesia-Turiňo S, Febrero A, Jauregui O, Caldelas C, Araus JL, Bort J (2006) Detection and quantification of unbound phytochelatin 2 in plant extracts of Brassica napus grown with different levels of mercury. Plant Physiol 142:742–749

    Article  PubMed  PubMed Central  Google Scholar 

  • Jahan MS, Guo S, Baloch AR, Sun J, Shu S, Wang Y, Ahammed GJ, Kabir K, Roy R (2020) Melatonin alleviates nickel phytotoxicity by improving photosynthesis, secondary metabolism and oxidative stress tolerance in tomato seedlings. Ecotox Environ Safe 197:110593

    Article  CAS  Google Scholar 

  • Jiang M, Zhang J (2001) Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265–1273

    Article  CAS  PubMed  Google Scholar 

  • Kim YO, Bae HJ, Cho E, Kang H (2017) Exogenous glutathione enhances mercury tolerance by inhibiting mercury entry into plant cells. Front Plant Sci 8:683

    Article  PubMed  PubMed Central  Google Scholar 

  • Li X, Ahammed GJ, Hang XN, Zhang L, Yan P, Zhang LP, Fu JY, Han WY (2021) Melatonin-mediated regulation of anthocyanin biosynthesis and antioxidant defense confer tolerance to arsenic stress in Camellia sinensis L. J Hazard Mater 403:123922

    Article  CAS  PubMed  Google Scholar 

  • Liu Z, Chen B, Wang L, Urbanovich O, Nagorskaya L, Li X, Tang L (2020) A review on phytoremediation of mercury contaminated soils. J Hazard Mater 400:123–138

    Article  Google Scholar 

  • López-Hidalgo C, Meijón M, Lamelas L, Valledor L (2021) The rainbow protocol: a sequential method for quantifying pigments, sugars, free amino acids, phenolics, flavonoids and MDA from a small amount of sample. Plant Cell Environ 44:1977–1986

    Article  PubMed  Google Scholar 

  • Ma R, Xu S, Zhao Y, Xia B, Wang R (2016) Selection and validation of appropriate reference genes for quantitative real-time PCR analysis of gene expression in Lycoris aurea. Front Plant Sci 7:536

    Article  PubMed  PubMed Central  Google Scholar 

  • Mareri L, Parrotta L, Cai G (2022) Environmental stress and plants. Int J Mol Sci 23:5416

    Article  PubMed  PubMed Central  Google Scholar 

  • Meng DK, Chen J, Yang Z (2011) Enhancement of tolerance of Indian mustard (Brassica juncea) to mercury by carbon monoxide. J Hazard Mater 186:1823–1829

    Article  CAS  PubMed  Google Scholar 

  • Moustafa-Farag M, Almoneafy A, Mahmoud A, Elkelish A, Arnao MB, Li L, Ai S (2020a) Melatonin and its protective role against biotic stress impacts on plants. Biomolecules 10:54

    Article  CAS  Google Scholar 

  • Nabaei M, Amooaghaie R (2019) Nitric oxide is involved in the regulation of melatonin- induced antioxidant responses in Catharanthus roseus roots under cadmium stress. Botany 97:681–690

    Article  CAS  Google Scholar 

  • Natasha SM, Khalid S, Bibi I, Bundschuh J, Niazi N, Dumat C (2020) A critical review of mercury speciation, bioavailability, toxicity and detoxification in soil-plant environment: ecotoxicology and health risk assessment. Sci Total Environ 711:134749

    Article  CAS  PubMed  Google Scholar 

  • Nawaz MA, Jiao Y, Chen C, Shireen F, Zheng Z, Imtiaz M, Bie Z, Huang Y (2018) Melatonin pretreatment improves vanadium stress tolerance of watermelon seedlings by reducing vanadium concentration in the leaves and regulating melatonin biosynthesis and antioxidant-related gene expression. J Plant Physiol 220:115–127

    Article  CAS  PubMed  Google Scholar 

  • Park J, Song WY, Ko D, Eom Y, Hansen TH, Schiller M, Lee TG, Martinoia E, Lee Y (2012a) The phytochelatin transporters AtABCC1 and AtABCC2 mediate tolerance to cadmium and mercury. Plant J 69:278–288

    Article  CAS  PubMed  Google Scholar 

  • Park S, Lee K, Kim YS, Back K (2012b) Tryptamine 5-hydroxylasedeficient Sekiguchi rice induces synthesis of 5-hydroxytryptophan and N-acetyltryptamine but decreases melatonin biosynthesis during senescence process of detached leaves. J Pineal Res 52:211–216

    Article  CAS  PubMed  Google Scholar 

  • Pasternak M, Lim B, Wirtz M, Hell R, Cobbett CS, Meyer AJ (2008) Restricting glutathione biosynthesis to the cytosol is sufficient for normal plant development. Plant J 53:999–1012

    Article  CAS  PubMed  Google Scholar 

  • Peng X, Wang N, Sun S, Geng L, Guo N, Liu A, Chen S, Ahammed GJ (2023) Reactive oxygen species signaling is involved in melatonin-induced reduction of chlorothalonil reside in tomato leaves. J Hazard Mater 443:130212

    Article  CAS  PubMed  Google Scholar 

  • Rahman I, Kode A, Biswas S (2006) Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nat Protoc 1:3159–3165

    Article  CAS  PubMed  Google Scholar 

  • Rajput VD, Hrish, Singh RK, Verma KK, Sharma L, Quiroz-Figueroa FR, Meena M, Gour VS, Minkina T, Sushkova S, Mandzhieva S (2021) Recent developments in enzymatic antioxidant defence mechanism in plants with special reference to abiotic stress. Biology 10:267

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rao MV, Chhunchha B (2010) Protective role of melatonin against the mercury induced oxidative stress in the rat thyroid. Food Chem Toxicol 48:7–10

    Article  CAS  PubMed  Google Scholar 

  • Safari F, Akramian M, Salehi-Arjmand H, Khadivi A (2019) Physiological and molecular mechanisms underlying salicylic acid-mitigated mercury toxicity in lemon balm (Melissa officinalis L). Ecotox Environ Safe 183:109542

    Article  CAS  Google Scholar 

  • Sarafi E, Tsouvaltzis P, Chatzissavvidis C, Siomos A, Therios I (2017) Melatonin and resveratrol reverse the toxic effect of high boron (B) and modulate biochemical parameters in pepper plants (Capsicum annuum L). Plant Physiol Biochem 112:173–182

    Article  CAS  PubMed  Google Scholar 

  • Shi S, Qiu Y, Li E, Wu L, Fu C (2006) Phylogenetic relationships and possible hybrid origin of Lycoris species (Amaryllidaceae) revealed by its sequences. Biochem Genet 44:198–208

    Article  CAS  PubMed  Google Scholar 

  • Sobrino-Plata J, Meyssen D, Cuypers A, Escobar C, Hernández LE (2014) Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil 377:369–381

    Article  CAS  Google Scholar 

  • Sun B, Wang P, Wang R, Li YK, Xu S (2018) Molecular cloning and characterization of a meta/para-O-methyltransferase from Lycoris aurea. Int J Mol Sci 19:1911

    Article  PubMed  PubMed Central  Google Scholar 

  • Sun C, Lv T, Huang L, Liu X, Jin C, Lin X (2020) Melatonin ameliorates aluminum toxicity through enhancing aluminum exclusion and reestablishing redox homeostasis in roots of wheat. J Pineal Res 68:e12642

    Article  CAS  PubMed  Google Scholar 

  • Sun C, Liu L, Wang L, Li B, Jin C, Lin X (2021) Melatonin: a master regulator of plant development and stress responses. J Integr Plant Biol 63:126–145

    Article  CAS  PubMed  Google Scholar 

  • Sytar O, Kumar A, Latowski D, Kuczynska P, Strzałka K, Prasad MNV (2013) Heavy metal-induced oxidative damage, defense reactions, and detoxification mechanisms in plants. Acta Physiol Plant 35:985–999

    Article  CAS  Google Scholar 

  • Vatamaniuk OK, Mari S, Lu Y, Rea PA (2000) Mechanism of heavy metal ion activation of phytochelatin (PC) synthase. J Biol Chem 275:31451–31459

    Article  CAS  PubMed  Google Scholar 

  • Wang F, Zeng B, Sun Z, Zhu C (2009) Relationship between proline and Hg2+-induced oxidative stress in a tolerant rice mutant. Arch Environ Contam Toxicol 56:723

    Article  CAS  PubMed  Google Scholar 

  • Wang R, Xu S, Jiang Y, Jiang J, Li X, Liang L, He J, Peng F, Xia B (2013) De novo sequence assembly and characterization of Lycoris aurea transcriptome using GS FLX Titanium platform of 454 pyrosequencing. PLoS ONE 8:e60449

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang R, Xu S, Wang N, Xia B, Jiang Y, Wang R (2017) Transcriptome analysis of secondary metabolism pathway, transcription factors, and transporters in response to methyl jasmonate in Lycoris aurea. Front Plant Sci 7:1971

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang P, Yu S, Han X, Xu J, He Q, Xu S, Wang R (2020) Identification, molecular characterization and expression of JAZ genes in Lycoris aurea. PLoS ONE 15:e0230177

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang K, Xing Q, Ahammed GJ, Zhou J (2022) Functions and prospects of melatonin in plant growth, yield, and quality. J Exp Bot 73:5928–5946

    Article  PubMed  Google Scholar 

  • Wu FH, Shen SC, Lee LY, Lee SH, Chan MT, Li CS (2009) Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method. Plant Methods 5:16

    Article  PubMed  PubMed Central  Google Scholar 

  • Xie C, Xiong X, Huang Z, Sun L, Ma J, Cai S, Yu F, Zhong W, Chen S, Li X (2018) Exogenous melatonin improves lead tolerance of bermudagrass through modulation of the antioxidant defense system. Int J Phytoremediat 20:1408–1417

    Article  CAS  Google Scholar 

  • Xu T, Chen Y, Kang H (2019) Melatonin is a potential target for improving post-harvest preservation of fruits and vegetables. Front Plant Sci 10:1388

    Article  PubMed  PubMed Central  Google Scholar 

  • Xu L, Zhang F, Tang M, Wang Y, Dong J, Ying J, Chen Y, Hu B, Li C, Liu L (2020) Melatonin confers cadmium tolerance by modulating critical heavy metal chelators and transporters in radish plants. J Pineal Res 69:e12659

    Article  CAS  PubMed  Google Scholar 

  • Yang B, Li Y, Song Y, Wang X, Guo Q, Zhou L, Xue X (2022) Zhang C (2022) The R2R3–MYB transcription factor VcMYB4a inhibits lignin biosynthesis in blueberry (Vaccinium corymbosum). Tree Genet Genomes 18(3):27

    Article  CAS  Google Scholar 

  • Zhang J, Li D, Wei J, Ma W, Kong X, Rengel Z, Chen Q (2019) Melatonin alleviates aluminum-induced root growth inhibition by interfering with nitric oxide production in Arabidopsis. Environ Exp Bot 161:157–165

    Article  CAS  Google Scholar 

  • Zhao D, Yao Z, Zhang J, Zhang R, Mou Z, Zhang X, Li Z, Feng X, Chen S, Reiter RJ (2021) Melatonin synthesis genes N-acetylserotonin methyltransferases evolved into caffeic acid O-methyltransferases and both assisted in plant terrestrialization. J Pineal Res 71:e12737

    Article  CAS  PubMed  Google Scholar 

  • Zhou ZS, Guo K, Elbaz AA, Yang ZM (2009) Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa. Environ Exp Bot 65:27–34

    Article  CAS  Google Scholar 

  • Zhou J, Obrist D, Dastoor A, Jiskra M, Ryjkov A (2021) Vegetation uptake of mercury and impacts on global cycling. Nat Rev Earth Environ 2:269

    Article  Google Scholar 

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Acknowledgements

The authors would like to thank Weicong Qi for the help in analyzing the Hg contents at the Jiangsu Academy of Agricultural Sciences.

Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31902063; 3207153), the “333 project” of Jiangsu Province (Grant No. BRA2020082).

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  1. Sheng Xu, Bin Sun and Junde Li have contributed equally to this work.

Authors and Affiliations

  1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, People’s Republic of China

    Sheng Xu, Bin Sun, Junde Li, Junya Xu, Wei-Kang Chen, Yumei Jiang, Jie Li, Zhe Zhou & Ren Wang

  2. Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Nanjing, 210014, People’s Republic of China

    Sheng Xu, Bin Sun, Yumei Jiang, Jie Li & Ren Wang

  3. Nanjing University of Chinese Medicine, Nanjing, 210023, People’s Republic of China

    Junde Li & Ren Wang

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  1. Sheng Xu
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by SX, BS, JL, JX, YJ, JL and ZZ. The first draft of the manuscript was written by SX, BS, WKC, and RW, Review and revision of the manuscript was performed by SX, BS, JL and RW. All authors read and approved the final manuscript.

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Correspondence to Ren Wang.

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Xu, S., Sun, B., Li, J. et al. Overexpression of caffeic acid O-methyltransferase from Lycoris aurea (LaCOMT) confers tolerance to mercury in Arabidopsis thaliana by modulating ROS homeostasis. Plant Growth Regul (2024). https://doi.org/10.1007/s10725-024-01133-1

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