Abstract
Brassinosteroids (BRs) and abscisic acid (ABA) both play positive roles in plant resistance to cold stress. Despite the recent report on the involvement of ABA in BR-induced enhanced tolerance to cold stress, the underlying molecular mechanisms of stress tolerance remain unclear. Moreover, whether there are ABA-independent pathways for BR-induced enhancement of cold stress tolerance in grapevines needs to be clarified. Herein, the potential involvement of ABA in BR-induced cold resistance in grapevines was investigated by contrasting the different responses among ABA, BR, and the combination of BR and NDGA (an inhibitor of endogenous ABA biosynthesis) treatments under cold stress. Results showed BR and ABA foliar application alone increased the chlorophyll fluorescence parameters, regulated the antioxidant system, and alleviated oxidative damage induced by cold stress. Interestingly, NDGA blocked the BR-induced cold resistance by increasing reactive oxygen species content and reducing antioxidant enzyme activity. Transcriptomic analysis suggested that exposure to cold stress resulted in very different patterns of gene expression and enriched pathway responses. Among them, ERF transcription factors were observed to be up-regulated in both BR and ABA treatment, calcium-binding protein genes were up-regulated only under BR treatment alone, and xyloglucosyl transferase genes were up-regulated only under ABA treatment. Overall, we concluded that ABA was involved in BR-induced cold resistance in grapevines, but there was also a different candidate pathway between ABA and BR treatments under cold stress.
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This work was supported by the Key R&D Programme Projects of Shaanxi Province of China (2022NY-113) and the China Agriculture Research System for Grape (CARS-29-zp-6).
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ZX designed the experiments and acquired the funding. ZC and YW performed the entire experimental work. SD reviewed and edited the paper. QJ and JZ analyzed the data. YW, BD and SD prepared the manuscript. XW edited the manuscript. The paper has been read and approved by all authors.
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Wang, Y., Ding, S., Chen, Z. et al. Transcriptomic analysis provides insights into the abscisic acid mediates brassinosteroid-induced cold resistance of grapevine (Vitis vinifera L.). Plant Growth Regul 101, 845–860 (2023). https://doi.org/10.1007/s10725-023-01060-7
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DOI: https://doi.org/10.1007/s10725-023-01060-7