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Ethylene and polyamines interact in rice spikelet degeneration in response to water stress during meiosis

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Abstract

This study aimed to investigate whether an interaction existed between ethylene and polyamines (PAs), which mediated the effects of water stress (WS) during meiosis on rice (Oryza sativa L.) spikelet degeneration. Two indica hybrid rice cultivars, HY113 (a drought-resistant rice cultivar) and ZZY8 (a drought-susceptible rice cultivar), were used in the present study, which were exposed to two soil moisture treatments: well water (WW) and WS. The spikelet degeneration rate (SDR) in WS plants increased by 12.1% for HY113 and 29.5% for ZZY8 compared with WW plants. The concentration of free spermidine (Spd) and free spermine (Spm) and the activities of S-adenosyl-l-methionine decarboxylase and Spd synthase were all significantly reduced in young panicles by the WS, with more reduction for ZZY8 than for HY113. The ethylene evolution rate, the concentrations of putrescine (Put), 1-aminocylopropane-1-car-boxylic acid (ACC), and H2O2, and the activities of ACC synthase and ACC oxidase in young panicles significantly increased for both cultivars under WS treatment, with more increase for ZZY8 than for HY113. Furthermore, when Spd or aminoethoxyvinylglycine (an inhibitor of ethylene synthesis) was applied to the young panicles of WS plants in the pollen mother cell meiosis prophase, the SDR significantly reduced. An opposite effect was observed when ACC or methylglyoxal-bis(guanylhydrazone) (an inhibitor of Spd and Spm synthesis) was applied to young panicles of WS plants. The results of the present study suggested the existence of an antagonistic interaction between ethylene and PA biosynthesis, which likely mediated the effect of WS during meiosis on rice spikelet degeneration.

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Abbreviations

ACC:

1-aminocylopropane-1-carboxylic acid

ADC:

Arginine decarboxylase

AVG:

Aminoethoxyvinylglycine

MGBG:

Methylglyoxal-bis (guanylhydrazone)

ODC:

Ornithine decarboxylase

PA(s):

Polyamine(s)

Put:

Putrescine

ROS:

Reactive oxygen species

SAM:

S-adenosyl-l-methionine

SAMDC:

S-adenosyl-l-methionine decarboxylase

SDR:

Spikelet degeneration rate

SWP:

Soil water potential

Spd:

Spermidine

Spm:

Spermine

WS:

Water stress

WW:

Well water

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Acknowledgements

This study was supported by the Key Research and Development Program of Zhejiang Province (2022C02034), the Natural Science Foundation of Zhejiang Province (LQ19C130008), the National Natural Science Foundation of China (32101825), Zhejiang “Ten thousand talents” plan science and technology innovation leading talent project (2020R52035) and the National Rice Industry Technology System (CARS-01).

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Authors and Affiliations

  1. China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, Zhejiang Province, China

    Guang Chu, Song Chen, Chunmei Xu, Yuanhui Liu, Xiufu Zhang & Danying Wang

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  1. Guang Chu
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Correspondence to Danying Wang.

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Communicated by Zhong-Hua Chen.

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Chu, G., Chen, S., Xu, C. et al. Ethylene and polyamines interact in rice spikelet degeneration in response to water stress during meiosis. Plant Growth Regul 101, 617–628 (2023). https://doi.org/10.1007/s10725-023-01043-8

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