Abstract
Salinity is the major cause of reducing crop yield in wheat, an important staple food crop for food security. Since the rhizosphere microbiome plays an important role in plant growth and development, the present study was conducted to characterize functional metabolic changes in the rhizosphere microbiota of wheat grown under saline and non-saline soils using comparative metaproteomics. In total 1538 and 891 proteins were obtained from wheat rhizosphere from saline and non-saline soils, respectively. The proteins DNA-directed RNA polymerase subunit beta’ (48.43%) followed by Leucine-tRNA ligase (4.45%) and translocase subunit SecA (2.69%) were relatively most abundantly present in salt stressed wheat rhizosphere metaproteome. Induced accumulation of proteins related to proline and spermidine biosynthesis was found in saline wheat rhizosphere. Inositol transporter involved in the osmotic balance and HSP90A, a key player to response regulator in stress were present in saline rhizosphere but were absent in non-saline conditions. Among 1410 proteins unique for saline soil, those linked predominantly with the pathways were sphingolipid, phosphinate and phenazine metabolism. The data is available in ProteomeXchange with the identifier PXD015387. The present study extends knowledge about the rhizosphere community functions utilizing a metaproteomic approach in wheat growing under saline conditions and can help in characterizing key proteins that may lead to physiological adaptations of the plants under saline environment.
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Data Availability
The mass spectrometry proteomics data have been deposited with the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD015387.
Submission details: Project Name: Metaproteomic datasets of wheat rhizosphere from saline and non-saline soils.
Project accession: PXD015387. Project DOI: Not applicable; Reviewer account details: Username: reviewer12163@ebi.ac.uk Password: 2Y0P1T2L.
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Acknowledgements
This work is supported by ICAR funded Network Project of the Center of Agricultural Bioinformatics (CABin) at ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM), Mau, and is work of Sub-Project “Metatranscriptomics and metaproteomics analysis of wheat rhizosphere”. The authors are thankful to the CABin, ICAR-IASRI project from the Indian Council of Agricultural Research, Government of India for financial support. The authors are grateful to the Director, ICAR-NBAIM, Mau for providing the necessary support to carry out the project and Dr Murugan Kumar, Scientist, ICAR-NBAIM for help in editing the manuscript.
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Renu, Sarim, K.M., Gupta, S.K. et al. A comparative analysis of rhizospheric metaproteome of wheat grown in saline and non-saline soils identifies proteins linked with characteristic functions. Plant Growth Regul 101, 415–426 (2023). https://doi.org/10.1007/s10725-023-01027-8
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DOI: https://doi.org/10.1007/s10725-023-01027-8