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Heat-induced RING/U-BOX E3 ligase, TaUHS, is a negative regulator by facilitating TaLSD degradation during the grain filling period in wheat

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Abstract

Heat stress accelerates abnormal maturation and leads to numerous defects in wheat. Temperature determines the duration of plant growth and heat stress accelerates crop development, resulting in premature initiation of phenological stages. The grain filling period is sensitive and is easily exposed to heat stress, which reduces the grain weight and shortens the period. In this study, from anthesis through early spike maturity, we observed decreased grain weight and accelerated maturation under heat stress, resulting in poor grain development. Furthermore, the maturation of spikes was accelerated to shorten the grain filling and ripening periods under high temperatures. Triticum aestivum L. UBOX responding Heat Stress gene (TaUHS) (TraesCS1D02G145900, UBOX response heat stress) was highly expressed in grains located at the basal positions of the spike under high-temperature stress. TaUHS is an E3 ligase found in the nucleus and plasma membrane. We demonstrated that TaUHS interacts with Triticum aestivum L. LEA containing seed development gene (TaLSD) (TraesCS5A02G385600, LEA-containing seed development). TaLSD, which played stress resistance and desiccation tolerance at grain filling period, mediates ubiquitination and proteasomal degradation by 26S proteasome with TaUHS under high-temperature conditions. Proteolysis of TaLSD in conjugate with E3 ligase might occur to maintain cellular homeostasis and stability in response to unfavorable environmental conditions. Wheat RING/U-BOX E3 ligase negatively regulates late embryogenesis-abundant proteins during the grain filling period under heat stress.

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Data availability

The FASTQ fles of raw data were uploaded to the NCBI Sequence Read Archive (SRA), and the SRA study accession is PRJNA877088.

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Acknowledgements

This work was supported by a Korea University grant.

Funding

Funding was provided by Cooperative Research Program for Agriculture Science & Technology Development (Grant No. PJ015705), Basic Science Research Program through the National Research Foundation of Korea (Grant No. 2019R1I1A1A01059968, NRF), Ministry of Science, ICT & Future Planning (Grant No. 2017R1D1A1B06030349).

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Authors and Affiliations

  1. Department of Plant Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea

    Chan Seop Ko, Joo Sun Lee & Yong Weon Seo

  2. Department of Plant Resources, College of Industrial Sciences, Kongju National University, 54, Daehak-ro, Yesan-eup, Yesan-gun, Chungnam, 32439, Republic of Korea

    Dae Yeon Kim

  3. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu, Jeongeup, 56212, Republic of Korea

    Jin-Baek Kim & Min Jeong Hong

  4. Ojeong Plant Breeding Research Center, Korea University, Seoul, 136-701, Republic of Korea

    Yong Weon Seo

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Contributions

CSK and YWS conceived and designed the study. CSK and J-BK designed the experiments to treat the plants. CSK, JSL, and MJH conducted the experiments. CSK and DYK contributed to analytical tools. CSK analyzed the data and wrote the first draft of the manuscript. All authors commented on previous versions of the manuscript. All authors read and approved the manuscript.

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Correspondence to Yong Weon Seo.

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Ko, C.S., Kim, D.Y., Lee, J.S. et al. Heat-induced RING/U-BOX E3 ligase, TaUHS, is a negative regulator by facilitating TaLSD degradation during the grain filling period in wheat. Plant Growth Regul 99, 251–264 (2023). https://doi.org/10.1007/s10725-022-00902-0

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