Abstract
Salinity stress is one of the major abiotic stresses that hampers the growth and productivity of plants. One of the sustainable approaches offering advantages over traditional bioinoculants is microbiome-based rhizospheric engineering for improving agricultural productivity and stress mitigation. Native members of the rhizospheric microbiome under stress, adapt to it over successive passaging, exerting a positive impact on plant’s fitness. The objective of this study was to use a multi-passaging approach for assessing the potential of an acclimatized rhizospheric microbiome in salinity stress mitigation in Vigna radiata. The novelty of this study lies in the ramping-up of salinity stress after every alternate passaging, and monitoring the efficacy of acclimatized microbiome in enabling plants to adapt to increasing levels of salinity. Further 16S rRNA amplicon sequencing was employed to assess the dynamics in the rhizospheric bacterial community under increasing stress levels over successive passages. The amendment with acclimatized microbiome led to improved plant growth attributes, as well as reduced levels of stress markers in Vigna radiata across the passaging cycles. Metagenomic analysis reflected distinct shifts in the rhizospheric microbiome, which correlated with the positive impact of acclimatised microbiome on enhanced plant development and lower levels of stress markers in plants, thereby aiding in salinity stress mitigation in the plant. This study highlights the importance of an acclimatized microbiome in salinity stress mitigation under increasing salinity levels in Vigna radiata over successive passaging cycles. Such a top-down approach of rhizospheric engineering is advocated for sustainable agriculture.
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Acknowledgements
SD wishes to acknowledge the fellowship received from Indian Institute of Technology Delhi, New Delhi, India. SK wishes to acknowledge the fellowship received from University Grants Commission, India. The authors thank Dr. Y. S. Shivay, Division of Agronomy, ICAR-IARI for help with estimation of ions.
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SD received fellowship from Indian Institute of Technology Delhi, India.
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SS conceptualized the idea and supervised the study, SD, SK and AB performed the experiments, SD analysed the data, SD and SS interpreted the results, and wrote the manuscript. All authors approved the manuscript.
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Dubey, S., Khatri, S., Bhattacharjee, A. et al. Multiple Passaging of Rhizospheric Microbiome Enables Mitigation of Salinity Stress in Vigna Radiata. Plant Growth Regul 97, 537–549 (2022). https://doi.org/10.1007/s10725-022-00820-1
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DOI: https://doi.org/10.1007/s10725-022-00820-1