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OsCRS2 encoding a peptidyl-tRNA hydrolase protein is essential for chloroplast development in rice

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Abstract

Chloroplasts are identified as important organelles for photosynthesis, biosynthesis, and storage of metabolites. So far, the molecular regulatory mechanism of chloroplast development remains to be unclear. The chloroplast RNA splicing 2 (CRS2) protein was determined to play a key role in chloroplast development as they affect the splicing of chloroplast gene introns in maize. However, the functions of OsCRS2 in rice chloroplast development have rarely been studied. In this study, oscrs2-1 and oscrs2-2 mutants were obtained to gene function research using the CRISPR/Cas9 gene editing technology. Both of the mutants displayed an albino seedling phenotype. The OsCRS2 is located in the chloroplast, and is highly expressed in green tissues. OsCRS2 could affect group I and seven group II introns splicing and participate in chloroplast rRNA biogenesis. RNA sequencing analysis indicated that both nuclear-encoded photosynthesis and chloroplast development-related genes expression were significantly reduced in the oscrs2-1 mutant. To conclude, these results suggested that OsCRS2 is necessary for early chloroplast development in rice.

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Acknowledgements

This research was supported by the ‘Collaborative Innovation Project’ of the Chinese Academy of Agricultural Sciences, the Central Public-interest Scientific Institution Basal Research Fund of China National Rice Research Institute (2017RG001-4), and the National Natural Science Foundation of China (31801333).

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Author notes

  1. Qiang Zhang, Yaliang Wang and Lan Shen contributed equally to this study.

Authors and Affiliations

  1. State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China

    Qiang Zhang, Yaliang Wang, Lan Shen, Deyong Ren, Jiang Hu, Li Zhu, Guangheng Zhang, Longbiao Guo, Dali Zeng & Qian Qian

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  1. Qiang Zhang
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  2. Yaliang Wang
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  3. Lan Shen
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Zhang, Q., Wang, Y., Shen, L. et al. OsCRS2 encoding a peptidyl-tRNA hydrolase protein is essential for chloroplast development in rice. Plant Growth Regul 92, 535–545 (2020). https://doi.org/10.1007/s10725-020-00655-8

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