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Arabidopsis CaM3 inhibits nitric oxide accumulation and improves thermotolerance by promoting S-nitrosoglutathione reductase via direct binding

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Abstract

Calmodulin 3 (CaM3) is a key component of heat shock (HS) signal transduction in Arabidopsis (Arabidopsis thaliana). We previously reported that nitric oxide (NO) acts as an upstream signal of AtCaM3 in plant thermotolerance. In this study, we demonstrated the effect of AtCaM3 on NO accumulation in the tolerance of Arabidopsis plants to HS. In vivo and in vitro protein–protein interaction assays displayed a direct binding between AtCaM3 and S-nitrosoglutathione reductase (GSNOR), inducing an increased GSNOR activity and an inhibited NO level. The cam3 mutant seedlings exhibited a high internal NO accumulation under HS. A gsnor deficiency decreased the survival ratio of cam3 plants accompanied by an increased internal NO level. However, treatment with 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (cPTIO, a special NO scavenger) elevated the survival ratio of cam3 mutant seedlings under HS conditions. Collectively, these data suggest an interesting finding that AtCaM3 serves as a signal in plant thermotolerance by reducing excessive NO accumulation through the binding and promoting of GSNOR, suggesting a feedback inhibition between AtCaM3 and NO in thermotolerance.

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Acknowledgements

We thanks Dr. Daye Sun (Hebei Normal University) for providing the seeds used in this research. This work was supported by the Natural Science Foundation of China (Grant No. 31770297).

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  1. Xiaona Zhang and Wenxu Wang have contributed equally to this work.

Authors and Affiliations

  1. Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, Hebei Collaboration Innovation Center for Cell Signaling, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China

    Xiaona Zhang, Wenxu Wang, Xinmiao Kang & Liqun Zhao

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  1. Xiaona Zhang
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  2. Wenxu Wang
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  3. Xinmiao Kang
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  4. Liqun Zhao
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Author contributions

LZ conceived the project. XZ, WW and XK carried out experiments and analyzed the data. LZ revised and proofread the manuscript. All authors read and approved the final manuscript.

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Correspondence to Liqun Zhao.

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Zhang, X., Wang, W., Kang, X. et al. Arabidopsis CaM3 inhibits nitric oxide accumulation and improves thermotolerance by promoting S-nitrosoglutathione reductase via direct binding. Plant Growth Regul 90, 41–50 (2020). https://doi.org/10.1007/s10725-019-00552-9

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