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A role of ETR1 in regulating leaf petiole elongation mediated by elevated temperature in Arabidopsis

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Abstract

Temperature fluctuation profoundly affects the plant growth and development. In this study, we show that ethylene receptor ETR1 is involved in regulating leaf petiole elongation mediated by higher temperatures (at 32 °C in this study). ETR1 loss-of-function mutant etr1-7 cannot elongate the leaf petiole at 32 °C as much as wild-type seedlings (WT). Overexpression of ETR1 in etr1-7 not only fully rescued the deficient in petiole elongation under higher temperature conditions but also caused longer petiole length under normal temperature conditions (22 °C). Plants with different mutant ETR1 alleles including etr1-7 etr1-1, and etr1-9 but not etr1-3 impair the petiole elongation mediated by elevated temperature. RNA-Seq analysis showed that hundreds of genes induced by elevated temperature in WT were not differentially expressed in etr1-7. Gene ontology enrichment analysis reveals that the molecular functions of these genes primarily relate to photosynthesis and protein degradation. Furthermore, genes involved in regulating organ elongation (such as BRI1-EMS-SUPPRESSOR 1, BES1), are significantly up-regulated in WT rather than in etr1-7 after the treatment of higher temperature. The results from this study suggest ETR1 is involved in regulating Arabidopsis response to elevated ambient temperature in both molecular and morphological levels.

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Acknowledgements

We thank E. Schaller (Dartmouth College, Hanover, NH) for the ETR1 full-length construct. Funding for this study was provided by the National Natural Science Foundation of China (31400248), the Jiangsu Science Fund for Distinguished Young Scholars, China (BK20150027), Natural Science Foundation of Jiangsu Province (Grant No. BK20160584), and the China State Key Laboratory of Plant Physiology and Biochemistry (Grant No. SKLPPBKF1507).

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Authors and Affiliations

  1. Institute of Crop Germplasm and Biotechnology, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Yuancong Wang, Bing He, Lihua Ning & Han Zhao

  2. College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing, 210095, China

    Chao-Feng Huang

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  1. Yuancong Wang
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  2. Bing He
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  3. Lihua Ning
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  4. Chao-Feng Huang
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  5. Han Zhao
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Correspondence to Han Zhao.

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Wang, Y., He, B., Ning, L. et al. A role of ETR1 in regulating leaf petiole elongation mediated by elevated temperature in Arabidopsis. Plant Growth Regul 86, 311–321 (2018). https://doi.org/10.1007/s10725-018-0430-4

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  • DOI: https://doi.org/10.1007/s10725-018-0430-4

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