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Lsi1-regulated Cd uptake and phytohormones accumulation in rice seedlings in presence of Si

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Abstract

Silicon (Si) is known for its role in regulating the response of plants to imposed abiotic stresses. Since the stresses generally hinder production of a crop, such as rice, the exploration of the biochemistry and plant physiology relating to the function is of interest. Indeed, recently, there were reports on the function of Lsi1 in regulating the tolerance of rice to cadmium (Cd) stress. This study compared the kinetics of the Cd uptakes in Lemont wild type rice and its transgenic lines exposed to Cd with or without exogenous Si supply. At the same time, changes on the endogenous phytohormones and growth of the rice seedlings were monitored. Genetically, Lsi1 overexpression was found to downregulate Km and Vmax of Cd uptake kinetics in the plants under Cd stress, especially in the presence of Si. On the other hand, Lsi1 RNAi upregulated Km and Vmax regardless whether Si was present or not. It implied that Lsi1 could be capable of regulating Si as well as Cd transports. Under Cd stress, addition of Si reduced the Cd uptake of the rice lines in the order of Lsi1-overexpression line > Lemont > Lsi1-RNAi line. In addition, it also affected the chlorophyll biosynthesis and dry mass accumulation of the rice plants under Cd stress. Analyses on phytohormones including IAA, GA3, JA, SA and ABA, as well as physiological functions, of the seedlings further verified the active involvement of Lsi1 in the complex defense system of the plants against Cd stress.

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Abbreviations

Si:

Silicon

Cd:

Cadmium

Lsi1 :

Low Si-influx 1

WT:

Lemont wild type rice

Lsi1-OE line:

Lsi1-overexpression transgenic Lemont rice

Lsi1-RNAi line:

Lsi1-RNAi transgenic Lemont rice

K m :

Michaelis constant

V max :

Maximum influx rate

IAA:

Indoleacetic acid

GA3 :

Gibberellin3

JA:

Jasmonic acid

SA:

Salicylic acid

ABA:

Abscisic acid

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Acknowledgements

This research work was supported by the National Natural Science Foundation of China (Nos. 31271670, 31300336), the National Research Foundation for the Doctoral Program of Higher Education of China (No. 20133515130001) and Fujian-Taiwan Joint Innovative Centre for Germplasm Resources and Cultivation of Crops (Fujian 2011 Program, 2015,75). We also acknowledged financial support for the project from Minjiang University.

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Authors and Affiliations

  1. Institute of Oceanography, Minjiang University, Fuzhou, 350108, People’s Republic of China

    Hongmei Lin

  2. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China

    Jianyu He, Weiwei Lin, Yingzhe Li, Changxun Fang & Wenxiong Lin

  3. Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou, 350002, People’s Republic of China

    Hongmei Lin, Jianyu He, Weiwei Lin, Yingzhe Li, Changxun Fang & Wenxiong Lin

  4. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China

    Wenxiong Lin

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  1. Hongmei Lin
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  2. Jianyu He
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  3. Weiwei Lin
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Correspondence to Changxun Fang or Wenxiong Lin.

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Lin, H., He, J., Lin, W. et al. Lsi1-regulated Cd uptake and phytohormones accumulation in rice seedlings in presence of Si. Plant Growth Regul 86, 149–157 (2018). https://doi.org/10.1007/s10725-018-0417-1

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  • DOI: https://doi.org/10.1007/s10725-018-0417-1

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