Effects of dark treatment and regular light recovery on the growth characteristics and regulation of chlorophyll in water dropwort
- 9 Downloads
Water dropwort is one of Apiaceae vegetables. Dark treatment could help to promote the degradation of chlorophyll and improve exterior quality and flavor of vegetable crops. Previous studies showed that the chlorophylls content would dramatically reduce in the dark, while the chlorophylls content would be promoted after regular light recovery. However, the understanding of the metabolic mechanism is limited in water dropwort. We treated the water dropwort under the dark at 0, 4, 8, 12, 16, 20 and 25 days, then, recovered with regular light for 2 and 4 days, respectively. The total of chlorophylls content gradually degraded and the chlorophyll a content decreased faster than chlorophyll b content during dark treatment in water dropwort. After regular light recovery, the expression levels of the genes related to chlorophyll synthesis and transformation were increased, while the expression levels of PPH and PAO degradation-related genes decreased gradually. The water dropwort sprouted a large amount of newborn petioles and leaf blades after 16 days dark treatment. After regular light recovery, chlorophyll content and gene expression level both increased slowly. The plants would maintain lower chlorophyll content for a long time and have a longer shelf-life after 16 days dark treatment. Taken together, the results suggested that the best time of blanching culture for water dropwort is 16 days. This study could help to elucidate the chlorophyll metabolism in water dropwort during blanching culture and provide new perspectives for screening the best time of dark treatment for water dropwort.
KeywordsWater dropwort Dark treatment Light recovery Chlorophyll Expression analysis Blanching culture
Chlorophyllide a oxygenase
Magnesium-chelatase subunit ChlH
Divinyl chlorophyllide a 8-vinyl-reductase
7-Hydroxymethyl chlorophyll a reductase
Magnesium-protoporphyrin IX monomethyl ester
Chlorophyll(ide) b reductase NOL
Chlorophyll(ide) b reductase NYC1
Pheophorbide a oxygenase
The research was supported by the National Natural Science Foundation of China (31272175); New Century Excellent Talents in University (NCET-11-0670); Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Conceived and designed the experiments: ASX and XYZ. Performed the experiments: XYZ, TL, GFT, YH and FW. Analyzed the data: XYZ, TL and ASX. Contributed reagents/materials/analysis tools: ASX. Wrote the paper: XYZ. Revised the paper: ASX, XYZ and TL. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Dongjin C, Changbae K, Sukhee L, Jaetak Y, Boosull C, Hyungkook K (2000) Effects of precooling and packaging film materials on quality of water dropwort (Oenanthe stolonifera DC.) at low temperature storage. J Kor Soc Hortic Sci:379–382Google Scholar
- Gonçalves EM, Pinheiro J, Abreu M, Brandão TRS, Silva CLM (2005) Influence of blanching treatments on colour, texture, chlorophylls content and sensory quality of broccoli (Brassica oleracea L.). ACS Appl Mater Interfaces 5(15):7291–7298Google Scholar
- Lee MS, Youn SY, Sang CY, Park HJ, Shin JD (1998) Bioremediation of heavy metals from the land application of industrial sewage sludge with minari (Oenanthe stolonifer DC.) plant. Plant Resourc 1:53–59Google Scholar
- Macalalad EA, Robidillo CJT, Marfori EC (2004) Influence of different cytokinins on the growth, -gingerol production and antioxidant activity of in vitro multiple shoot culture of ginger (Zingiber officinale Roscoe). Res J Med Plant 10:194–200Google Scholar
- Pružinská A, Tanner G, Aubry S, Anders I, Moser S, Müller T, Ongania KH, Kräutler B, Youn JY, Liljegren SJ (2005) Chlorophyll breakdown in senescent Arabidopsis leaves: characterization of chlorophyll catabolites and of chlorophyll catabolic enzymes involved in the degreening reaction. Plant Physiol 139:52–63CrossRefPubMedPubMedCentralGoogle Scholar
- Wang YW, Xu C, Wu M, Chen GX (2016) Characterization of photosynthetic performance during reproductive stage in high-yield hybrid rice LYPJ exposed to drought stress probed by chlorophyll a fluorescence transient. Plant Growth Regul 81(3):1–11Google Scholar
- Zhang X, Zhang Z, Li J, Wu L, Guo J, Ouyang L, Xia Y, Huang X, Pang X (2011) Correlation of leaf senescence and gene expression/activities of chlorophyll degradation enzymes in harvested Chinese flowering cabbage (Brassica rapa var. parachinensis). J Plant Physiol 168:2081–2087CrossRefPubMedGoogle Scholar
- Zhu MK, Meng XQ, Chen GP, Dong TT, Yu XH, Cai J, Hu ZL (2016) Physiological, biochemical, and molecular differences in chloroplast synthesis between leaf and corolla of cabbage (Brassica rapa L. var. chinensis) and rapeseed (Brassica napus L.). Plant Growth Regul 82:1–11Google Scholar