Plant Growth Regulation

, Volume 85, Issue 2, pp 281–292 | Cite as

Overexpression of HvCBF7 and HvCBF9 changes salt and drought tolerance in Arabidopsis

  • Shuya Yin
  • Yong Han
  • Lu Huang
  • Ye Hong
  • Guoping Zhang
Original paper


Crop yield losses due to the extreme environments have risen steadily over the past several decades, so it is quite imperative to develop the crop cultivars with high stress tolerance for ensuring global food security. Dehydration responsive element binding protein/C-repeat binding factor (DREB/CBF) transcription factors are widely concerned as key regulators, specifically in abiotic tolerance of plants. In this study, we found that barley CBF7 and CBF9 were induced by salt and drought stress. Over-expression of HvCBF7 and HvCBF9 enhanced salt tolerance, showing increased survival rate of Arabidopsis seedlings under salt stress, but reduced drought and salt tolerance in adult plants before flowering stage, as reflected by higher malondialdehyde and proline contents. The altered phenotype was due to differently regulating stress response genes. In addition, we also found that many genes expressing differently under salt stress were reversely regulated by AtCBF3, a transcription factor in Arabidopsis.


Arabidopsis Drought Regulation Salt Tolerance Transcription factor 



This work was supported by Natural Science Foundation of China (31620103912), China Agriculture Research System (CARS-05) and Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP). Tibetan wild barley accessions in this research was provided by Professor Dongfa Sun (Huazhong Agricultural University, China).

Compliance with ethical standards

Conflict of interest

The authors have declared that no competing interests exist.

Supplementary material

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Supplementary material 1 (DOCX 1252 KB)
10725_2018_394_MOESM2_ESM.docx (19 kb)
Supplementary material 2 (DOCX 19 KB)
10725_2018_394_MOESM3_ESM.xlsx (888 kb)
Supplementary material 3 (XLSX 888 KB)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Shuya Yin
    • 1
  • Yong Han
    • 1
  • Lu Huang
    • 1
  • Ye Hong
    • 1
  • Guoping Zhang
    • 1
  1. 1.Department of Agronomy, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China

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