New QTLs identified for leaf correlative traits in rice seedlings under cadmium stress
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Cadmium (Cd) is a non-essential toxic metal that is primarily released into the environment from artificial sources in recent decades. To investigate the genetics of Cd toxicity tolerance at the seedling stage in rice, a QTL analysis was carried out under cadmium stress conditions with two toxicity-linked traits—leaf rolling (LR) and the green leaf ratio (GLR). Using 127 rice lines of doubled haploid (DH) population derived from a cross between a japonica JX17 and indica ZYQ8, two QTLs for LR (qLR-1 and qLR-9) and one QTL for GLR (qGLR-3) were detected. Among them, the phenotypic variation of qLR-1 and qGLR-3 were 19.27 and 16.09, values which are useful for marker-assistant selection in breeding elite rice cultivars that have the capacity to tolerate Cd. The results further demonstrate that visual measurements of both LR and GLR in seedlings are effective methods for screening tolerant rice germplasm in cadmium stress scenarios.
KeywordsRice QTL Cadmium Leaf rolling Green leaf ration
This work was supported by Hangzhou Scientific and Technological Program (20170432B03), the National Key Technology Research and Development Program (2015BAD01B02) and the National Natural Science Foundation of China (31661143006). This work was also supported in part by the National GMO New Variety Breeding Program of PRC (2016ZX08011-001), National Science Foundation of China (31671666).
- Anjum SA, Tanveer M, Hussain S, Bao M, Wang L, Khan I, Ullah E, Tung SA, Samad RA, Shahzad B (2015) Cadmium toxicity in maize (Zea mays L.): consequences on antioxidative systems, reactive oxygen species and cadmium accumulation. Environ Sci Pollut Res Int 22(21):17022–17030CrossRefPubMedGoogle Scholar
- Huang DR, Fan YY, Hu BL, Xiao YQ, Chen DZ, Zhuang JY (2017) Assessment and genetic analysis of heavy metal content in rice grain using an Oryza sativa × O. rufipogon backcross inbred line population. J Sci Food Agric 7:17704Google Scholar
- Inoue H, Takahashi M, Kobayashi T, Suzuki M, Nakanishi H, Mori S, Nishizawa NK (2008) Identification and localisation of the rice nicotianamine aminotransferase gene OsNAAT1 expression suggests the site of phytosiderophore synthesis in rice. Plant Mol Biol 66(1–2):193–203CrossRefPubMedGoogle Scholar
- Ishikawa S, Ishimaru Y, Igura M, Kuramata M, Abe T, Senoura T, Hase Y, Arao T, Nishizawa NK, Nakanishi H (2012) Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice. Proc Natl Acad Sci USA 109(47):19166–19171CrossRefPubMedPubMedCentralGoogle Scholar
- Lang Y, Zhang Z, Gu X, Yang J, Zhu Q (2004) Physiological and ecological effects of crimpy leaf character in rice (Oryza sativa L.) II. Photosynthetic character, dry mass production and yield forming. Acta Agronomica Sin 30(9):883–887Google Scholar
- McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T (1997) Report on QTL nomenclature. Rice Genet Newslett 14:11–13Google Scholar
- Miyadate H, Adachi S, Hiraizumi A, Tezuka K, Nakazawa N, Kawamoto T, Katou K, Kodama I, Sakurai K, Takahashi H, Satoh NN, Watanabe A, Fujimura T, Akagi H (2011) OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles. New Phytol 189(1):190–199CrossRefPubMedGoogle Scholar
- Turner NC (1997) Further progress in crop water relations. Adv Agron 58(8):293–338Google Scholar
- Xu Y, Shen L, McCouch S, Zhu L (1998) Extension of the rice DH population genetic map with microsatellite markers. Chin Sci Bull 42:149–152Google Scholar
- Yoshida S, Forna DA, Cock JH (1976) Laboratory manual for physiological studies of rice. International Rice Research Institute, Los Banos, pp 62–63Google Scholar