Effects of artemisinin on root gravitropic response and root system development in Arabidopsis thaliana
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Artemisinin, an antimalarial secondary metabolite produced in Artemisia species, also has been recognized as an allelochemical that inhibits the growth of several plant species. However, the phytotoxicity mechanism of artemisinin is not exhaustively deciphered up to now. In this research, the effects of artemisinin on Arabidopsis thaliana root gravitropic curvature and development were characterized. Exogenously applied artemisinin disturb the root gravitropic responses, inhibited the elongation of primary and lateral roots and root hairs in a concentration-dependent fashion, and prevented the formation of lateral roots and root hairs. Moreover, the number of starch grain and the distribution range of auxin in the root tip was reduced by artemisinin, and the redistribution of auxin was less sensitive to gravity stimulus when treated with artemisinin than that of control. The expression of auxin transporter PIN2 was partially suppressed by artemisinin. Together, the results demonstrated that the effects of artemisinin on root gravitropism and root system development were largely dependent on the reduction of starch grain and auxin levels, as well as the disordered lateral auxin redistribution.
KeywordsArabidopsis thaliana Artemisinin Auxin Gravitropism Root development Starch grain
Median effective dose
Murashige and Skoog
Lateral root primordia
Reactive oxygen species
Root system architecture
This work was financially supported by the National Natural Science Foundation of China (31300290, 31570354 and 21302195), CAS “Light of West China” Program, The Science Foundations of Gansu Province (17JR5RA301), The Youth Science Foundations of Gansu Province (1506RJYA294) and 135 major breakthrough project of Chinese Academy of Sciences.
- Delabays N, Slacanin I, Bohren C (2008) Herbicidal potential of artemisinin and allelopathic properties of Artemisia annua L: from the laboratory to the field. J Plant Dis Protect Sp Iss 21:317–322Google Scholar
- Duke SO, Vaughn KC, Croom EM, ElSohly HN (1987) Artemisinin, a constituent of annual wormwood (Artemisia Annua), is a selective phytotoxin. Weed Sci 35:499–505Google Scholar
- Hasegawa T, Omiya Y, Koide M, Shigemori H, Ueda J, Hasegawa K, Miyamoto K (2017) A gravitropic stimulation-induced growth inhibitor, β-(isoxazolin-5-on-2yl)-alanine, is a possible mediator of negative gravitropic bending of epicotyls in etiolated Pisum sativum seedlings. Plant Growth Regul 82:431–438CrossRefGoogle Scholar
- Lydon J, Teasdale JR, Chen PK (1997) Allelopathic activity of annual wormwood (Artemisia annua) and the role of artemisinin. Weed Sci 45:807–811Google Scholar
- Panamanik RC, Chikkaswamy BK, Roy DG, Panamanik A, Kumar V (2008) Effects of biochemicals of Artemisia annua in plants. J Pierre Fauchard Acad 21:11–18Google Scholar
- Scheres B, Benfey P, Dolan L (2002) Root development. In: Somerville CR, Meyerowitz EM (eds), The Arabidopsis book. America Society of Plant Biologists, Rockville, p e0101Google Scholar