Rice DEAD-box RNA helicase OsRH53 has negative impact on Arabidopsis response to abiotic stresses
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DEAD-box RNA helicases (RHs) play key roles in the regulation of RNA metabolism at the posttranscriptional level. In this study, the expression patterns under abiotic stresses and the functions of a rice (Oryza sativa) RH, OsRH53, in stress response were determined using transgenic Arabidopsis plants. The level of OsRH53 decreased upon abiotic stress treatment, including, drought, salt, cold, and UV stress, and by abscisic acid (ABA). Although OsRH53 contains a putative chloroplast transit peptide at the N-terminal end, confocal analysis of OsRH53–GFP fusion proteins transiently expressed in tobacco leaves revealed that OsRH53 is localized to the nucleus. OsRH53-expressing transgenic Arabidopsis displayed retarded germination and reduced growth under high salinity or dehydration stress but not under cold stress. OsRH53 negatively affected the growth and cotyledon greening of seedlings upon ABA application by activating the genes related to ABA signaling such as ABI3 and ABI4. The ability of OsRH53 to recover growth-defect phenotype of Escherichia coli mutant, and both in vitro and in vivo base pair-breaking ability confirmed that OsRH53 harbors RNA chaperone activity. Collectively, these results suggest that OsRH53 negatively affects plant abiotic stress responses via modulating RNA metabolism through its RNA chaperone activity.
KeywordsAbiotic stress DEAD-box Rice RNA helicase
This work was supported by a grant from the Next-Generation BioGreen21 Program (PJ01103601; PJ01312201), Rural Development Administration, Republic of Korea.
- Grover A, Minhas D (2000) Towards the production of abiotic stress tolerant transgenic rice plants: Issues, progress and future research needs. Proc Indian Nat Sci Acad B 66:13–32Google Scholar
- Halls C, Mohr S, del Campo M, Yang Q, Jankowsky E, Lambowitz AM (2007) Involvement of DEAD-box proteins in group I and group II intron splicing. Biochemical characterization of Mss116p, ATP hydrolysis-dependent and -independent mechanisms, and general RNA chaperone activity. J Mol Biol 365:835–855CrossRefPubMedGoogle Scholar
- Hsu YF, Chen YC, Hsiao YC, Wang BJ, Lin SY, Cheng WH, Jauh GY, Harada JJ, Wang CS (2014) AtRH57, a DEAD-box RNA helicase, is involved in feedback inhibition of glucose-mediated abscisic acid accumulation during seedling development and additively affects pre-ribosomal RNA processing with high glucose. Plant J 77:119–135CrossRefPubMedGoogle Scholar
- Semrad K (2011) Proteins with RNA chaperone activity: a world of diverse proteins with a common task-impediment of RNA misfolding. Biochem Res Int 10:1155–1165Google Scholar
- Xu T, Gu L, Choi MJ, Kim RJ, Suh MC, Kang H (2014) Comparative functional analysis of wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding proteins in response to abiotic stresses. PLoS ONE 9:1–8Google Scholar
- Zhu M, Chen G, Dong T, Wang L, Zhang J, Zhao Z (2015) SlDEAD31, a putative DEAD-box RNA helicase gene, regulates salt and drought tolerance and stress-related genes in tomato. PLoS ONE 10:1–20Google Scholar