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Plant Growth Regulation

, Volume 85, Issue 1, pp 153–163 | Cite as

Rice DEAD-box RNA helicase OsRH53 has negative impact on Arabidopsis response to abiotic stresses

  • Ghazala Nawaz
  • Than Zaw Tun Sai
  • Kwanuk Lee
  • Yeon-Ok Kim
  • Hunseung Kang
Original paper
  • 215 Downloads

Abstract

DEAD-box RNA helicases (RHs) play key roles in the regulation of RNA metabolism at the posttranscriptional level. In this study, the expression patterns under abiotic stresses and the functions of a rice (Oryza sativa) RH, OsRH53, in stress response were determined using transgenic Arabidopsis plants. The level of OsRH53 decreased upon abiotic stress treatment, including, drought, salt, cold, and UV stress, and by abscisic acid (ABA). Although OsRH53 contains a putative chloroplast transit peptide at the N-terminal end, confocal analysis of OsRH53–GFP fusion proteins transiently expressed in tobacco leaves revealed that OsRH53 is localized to the nucleus. OsRH53-expressing transgenic Arabidopsis displayed retarded germination and reduced growth under high salinity or dehydration stress but not under cold stress. OsRH53 negatively affected the growth and cotyledon greening of seedlings upon ABA application by activating the genes related to ABA signaling such as ABI3 and ABI4. The ability of OsRH53 to recover growth-defect phenotype of Escherichia coli mutant, and both in vitro and in vivo base pair-breaking ability confirmed that OsRH53 harbors RNA chaperone activity. Collectively, these results suggest that OsRH53 negatively affects plant abiotic stress responses via modulating RNA metabolism through its RNA chaperone activity.

Keywords

Abiotic stress DEAD-box Rice RNA helicase 

Notes

Acknowledgements

This work was supported by a grant from the Next-Generation BioGreen21 Program (PJ01103601; PJ01312201), Rural Development Administration, Republic of Korea.

Supplementary material

10725_2018_381_MOESM1_ESM.pdf (392 kb)
Supplementary material 1 (PDF 392 KB)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Ghazala Nawaz
    • 1
  • Than Zaw Tun Sai
    • 1
  • Kwanuk Lee
    • 1
  • Yeon-Ok Kim
    • 1
  • Hunseung Kang
    • 1
  1. 1.Department of Plant Biotechnology, College of Agriculture and Life SciencesChonnam National UniversityGwangjuSouth Korea

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