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Effect of down-regulating 1-deoxy-d-xylulose-5-phosphate reductoisomerase by RNAi on growth and artemisinin biosynthesis in Artemisia annua L.

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Abstract

1-Deoxy-d-xylulose-5-phosphate reductoisomerase (DXR), an important enzyme in the 2-c-methyl-d-erythritol-4-phosphate (MEP) pathway in plant plastids, provides the basic five-carbon units for isoprenoid biosynthesis. To investigate the roles of the MEP pathway in regulating growth, development and artemisinin biosynthesis of Artemisia annua L., we used RNA interference technology to generate transgenic plants with suppressed expression of DXR in A. annua (AaDXR). Suppression of AaDXR resulted in shorter stems, decreased branch numbers and leaf area, lower density of leaf trichomes. Although AaDXR-RNAi plants had no significant changes on the stomatal conductance, the net photosynthesis rate was decreased by 20.0–31.4% due to the marked decline in the contents of chlorophyll. Decreased levels of endogenous gibberellic acid (GA3) and abscisic acid were also detected in the transgenic lines. The artemisinin contents in leaves of all tested transgenic lines declined by 41.8–73.4% at the vegetative stage and 61.5–63.6% at the stages of flowering. The enhancement of artemisinin contents by methyl jasmonate at 300 µM has been abolished at seedling and vegetative stages in AaDXR-RNAi plants. These results demonstrate that AaDXR play import roles in the control of plan vegetative growth and artemisinin biosynthesis in A. annua.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81273487, 81473183), Soochow Scholar Program (No. 14317363) and the Graduate Program of Higher Education in Jiangsu Province (No. CXLX13-841).

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Authors and Affiliations

  1. College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, China

    Cong Hui Wang, Xiu Yun Lei, Jing Xia & Jian Wen Wang

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Correspondence to Jian Wen Wang.

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Wang, C.H., Lei, X.Y., Xia, J. et al. Effect of down-regulating 1-deoxy-d-xylulose-5-phosphate reductoisomerase by RNAi on growth and artemisinin biosynthesis in Artemisia annua L.. Plant Growth Regul 84, 549–559 (2018). https://doi.org/10.1007/s10725-017-0360-6

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