Effect of paclobutrazol, a potential growth regulator on stalk mechanical strength, lignin accumulation and its relation with lodging resistance of maize Original paper First Online: 08 November 2017 Abstract
Dense plant cultivation is an efficient approach to utilize the maximum inputs for increasing maize production. However, dense plant populations may prone to lodging as it results in increased plant height and reduced culm diameter; therefore, we hypothesized that weaker stems may be responsible for maize lodging. In this study, we examined the regulatory effects of paclobutrazol under two commonly used application methods (seed-soaking and seed-dressing). Seed-soaking with paclobutrazol at the rate of 0 (CK1), 200 (S1), 300 (S2), and 400 (S3) mg L
−1, while seed-dressing at the rate of 0 (CK2), 1.5 (D1), 2.5 (D2), and 3.5 (D3) g kg −1 were used. Results showed that paclobutrazol improved the culm physical strength by increasing the rind penetration strength, stalk breaking strength, culm diameter, wall thickness, and dry weight per unit length of basal third internode, compared to control plants. Moreover, paclobutrazol reduced the internode length, plant height, ear height, center of gravity height and lodging rate in both growing seasons. In addition, more lignin was accumulated in the basal internode and the activities of phenylalanine ammonia-lyase (PAL), peroxidase (POD), cinnamyl alcohol dehydrogenase (CAD) and 4-coumarate: CoA ligase (4CL) increased with paclobutrazol, and their maximum values were observed in the S2 and D3 treatments, resulting in strong lodging resistance. Lignin content was positively and significantly correlated with the rind penetration strength, breaking strength of internode, and activities of PAL, 4CL, POD, and CAD, while significantly and negatively correlated with lodging percentage. The present findings suggested that 300 mg L −1 and 3.5 g kg −1 of paclobutrazol may efficiently be utilized to minimize the risk of lodging, not only by manipulating plant height but also by enhancing culm physical strength and lignin accumulation in basal internodes. Keywords Enzyme activities Lignin Lodging Paclobutrazol Stem strength Zea mays L.
Muhammad Kamran and Cui Wenwen have contributed equally to this work.
Han Qingfang and Liu Tiening jointly supervised this work.
A correction to this article is available online at
. https://doi.org/10.1007/s10725-018-0367-7 Notes Acknowledgements
This work was supported by High Technology Research and Development Program of China (863 Program, No. 2013AA102902), the Special Fund for Agro-scientific Research in the Public Interest (201303104), the 111 Project of Chinese Education Ministry (B12007).
Acreche MM, Slafer GA (2011) Lodging yield penalties as affected by breeding in Mediterranean wheats. F Crop Res 122:40–48.
https://doi.org/10.1016/j.fcr.2011.02.004 CrossRef Google Scholar
Assis JS, Maldonado R, Muñoz T, Escribano MI, Merodio C (2001) Effect of high carbon dioxide concentration on PAL activity and phenolic contents in ripening cherimoya fruit. Postharvest Biol Technol 23:33–39.
https://doi.org/10.1016/S0925-5214(01)00100-4 CrossRef Google Scholar
Berry PM, Spink J (2012) Predicting yield losses caused by lodging in wheat. F Crop Res 137:19–26.
https://doi.org/10.1016/j.fcr.2012.07.019 CrossRef Google Scholar
Berry PM, Spink J, Sterling M, Pickett AA (2003) Methods for rapidly measuring the lodging resistance of wheat cultivars. J Agron Crop Sci 189:390–401.
https://doi.org/10.1046/j.0931-2250.2003.00062.x CrossRef Google Scholar
Boudet AM, Kajita S, Grima-Pettenati J, Goffner D (2003) Lignins and lignocellulosics: a better control of synthesis for new and improved uses. Trends Plant Sci 8:576–581
CrossRef PubMed Google Scholar
Cai T, Xu H, Peng D, Yin Y, Yang W, Ni Y, Chen X, Xu C, Yang D, Cui Z, Wang Z (2014) Exogenous hormonal application improves grain yield of wheat by optimizing tiller productivity. F Crop Res 155:172–183.
https://doi.org/10.1016/j.fcr.2013.09.008 CrossRef Google Scholar
Chen XG, Shi CY, Yin YP, Wang ZL, Shi YH, Peng DL, Ni YL, Cai T (2011) Relationship between lignin metabolism and lodging resistance in wheat. Acta Agron Sin 37:1616–1622
CrossRef Google Scholar
Dixon RA (2001) Natural products and plant disease resistance. Nature 411:843–847
CrossRef PubMed Google Scholar
Dong XH, Duan LS, Meng FL, He ZP, Li ZH (2006) Effects of spraying 30% DTA-6. Ethephon solution on yield and straw quality of maize. J Maize Sci 14:138–140
Dong Z, Ma X, Wang Q, Lin Z, Xu J, Yan J, Wang C (2008) Effects of spraying Yu-Huang-Jin on maize yield. J Maize Sci 16:91–93
Esechie HA, Rodriguez V, Al-Asmi H (2004) Comparison of local and exotic maize varieties for stalk lodging components in a desert climate. Eur J Agron 21:21–30.
https://doi.org/10.1016/S1161-0301(03)00060-1 CrossRef Google Scholar
FAO (2012) World agriculture towards 2030/2050: the 2012 revision
Fletcher RA, Gilley A, Sankhla N, Davis TD (2000) Triazoles as plant growth regulators and stress protectants. Horticult Rev.
https://doi.org/10.1002/9780470650776.ch3 Google Scholar
Flint-Garcia SA, Darrah LL, McMullen MD, Hibbard BE (2003) Phenotypic versus marker-assisted selection for stalk strength and second-generation European corn borer resistance in maize. Theor Appl Genet 107:1331–1336
CrossRef PubMed Google Scholar
Guoping Z, Jianxing C, Bull DA (2001) The effects of timing of N application and plant growth regulators on morphogenesis and yield formation in wheat. Plant Growth Regul 35:239–245.
https://doi.org/10.1023/A:1014411316780 CrossRef Google Scholar
Hussain S, Peng S, Fahad S, Khaliq A, Huang J, Cui K, Nie L (2015) Rice management interventions to mitigate greenhouse gas emissions: a review. Environ Sci Pollut Res 22:3342–3360.
https://doi.org/10.1007/s11356-014-3760-4 CrossRef Google Scholar
Islam MS, Peng S, Visperas RM, Ereful N, Bhuiya MSU, Julfiquar AW (2007) Lodging-related morphological traits of hybrid rice in a tropical irrigated ecosystem. F Crop Res 101:240–248.
https://doi.org/10.1016/j.fcr.2006.12.002 CrossRef Google Scholar
Kawasaki T, Koita H, Nakatsubo T, Hasegawa K, Wakabayashi K, Takahashi H, Umemura K, Umezawa T, Shimamoto K (2006) Cinnamoyl-CoA reductase, a key enzyme in lignin biosynthesis, is an effector of small GTPase Rac in defense signaling in rice. Proc Natl Acad Sci USA 103:230–235
CrossRef PubMed Google Scholar
Khan W, Prithiviraj B, Smith DL (2003) Chitosan and chitin oligomers increase phenylalanine ammonia-lyase and tyrosine ammonia-lyase activities in soybean leaves. J Plant Physiol 160:859–863
CrossRef PubMed Google Scholar
Kong E, Liu D, Guo X, Yang W, Sun J, Li X, Zhan K, Cui D, Lin J, Zhang A (2013) Anatomical and chemical characteristics associated with lodging resistance in wheat. Crop J 1:43–49.
https://doi.org/10.1016/j.cj.2013.07.012 CrossRef Google Scholar
Lee D, Meyer K, Chapple C, Douglas CJ (1997) Antisense suppression of 4-coumarate:coenzyme A ligase activity in Arabidopsis leads to altered lignin subunit composition. Plant Cell 9:1985–1998.
https://doi.org/10.1105/tpc.9.11.1985 CrossRef PubMed PubMedCentral Google Scholar
Li N, Li JM, Zhai Z, Li ZH, Duan LS (2010) Effects of chemical regulator on the lodging resistance traits, agricultural characters and yield of maize. J Maize Sci 18:38–42.
https://doi.org/10.13597/j.cnki.maize.science.2010.06.015 Google Scholar
Liu Z, Gou L, Zhao M, Zhang B (2011) Effects of shading on stalk morphological characteristics rind penetration strength and lodging-resistance of maize. Acta Agric Boreali-Sinica 26:91–96
Liu XB, Dong-qian W, Can W, Dan H, Hao Y, Heng-zhi S, Ren-wu R, Xiao-hui Y, Ze-lin Y (2015) Effects of spraying uniconazole on lodging resistance of culm and yield in common buckwheat. Sci Agric Sin 48:4903–4915.
https://doi.org/10.3864/j.issn.0578-1752.2015.24.005 Google Scholar
Liu XB, Wang C, Hu D, Yang H, She HZ, Ruan RW, Wu DQ, Yi ZL (2016) Effects of seed dressing with uniconazole powder on lodging resistance of culm in common buckwheat. Acta Agron Sin.
https://doi.org/10.3724/SP.J.1006.2016.00093 Google Scholar
Ma QH (2009) The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance. J Exp Bot 60:2763–2771.
https://doi.org/10.1093/jxb/erp132 CrossRef PubMed PubMedCentral Google Scholar
Ma QH (2010) Functional analysis of a cinnamyl alcohol dehydrogenase involved in lignin biosynthesis in wheat. J Exp Bot 61:2735–2744
CrossRef PubMed PubMedCentral Google Scholar
Ma D, Xie R, Liu X, Niu X, Hou P, Wang K, Lu Y, Li S (2014) Lodging-related stalk characteristics of maize varieties in China since the 1950s. Crop Sci 54:2805–2814.
https://doi.org/10.2135/cropsci2014.04.0301 CrossRef Google Scholar
Maddonni GA, Otegui ME (2004) Intra-specific competition in maize: Early establishment of hierarchies among plants affects final kernel set. F Crop Res 85:1–13.
https://doi.org/10.1016/S0378-4290(03)00104-7 CrossRef Google Scholar
Moerschbacher BM, Noll UM, Flott BE, Reisener HJ (1988) Lignin biosynthetic enzymes in stem rust infected, resistant and susceptible near-isogenic wheat lines. Physiol Mol Plant Pathol 33:33–46.
https://doi.org/10.1016/0885-5765(88)90041-0 CrossRef Google Scholar
Novacek MJ, Mason SC, Galusha TD, Yaseen M (2013) Twin rows minimally impact irrigated maize yield, morphology, and lodging. Agron J 105:268–276.
https://doi.org/10.2134/agronj2012.0301 CrossRef Google Scholar
Okuno A, Hirano K, Asano K, Takase W, Masuda R, Morinaka Y, Ueguchi-Tanaka M, Kitano H, Matsuoka M (2014) New approach to increasing rice lodging resistance and biomass yield through the use of high gibberellin producing varieties. PLoS ONE 9:e86870.
https://doi.org/10.1371/journal.pone.0086870 CrossRef PubMed PubMedCentral Google Scholar
Özmen AD, Özdemir F, Türkan I (2003) Effects of paclobutrazol on response of two barley cultivars to salt stress. Biol Plant 46:263–268.
https://doi.org/10.1023/A:1022862929881 CrossRef Google Scholar
Peng D, Chen X, Yin Y, Lu K, Yang W, Tang Y, Wang Z (2014) Lodging resistance of winter wheat (
L.): Lignin accumulation and its related enzymes activities due to the application of paclobutrazol or gibberellin acid. F Crop Res 157:1–7.
https://doi.org/10.1016/j.fcr.2013.11.015 CrossRef Google Scholar
Plaza-Wüthrich S, Blösch R, Rindisbacher A, Cannarozzi G, Tadele Z (2016) Gibberellin deficiency confers both lodging and drought tolerance in small cereals. Front Plant Sci 7:1–14.
https://doi.org/10.3389/fpls.2016.00643 CrossRef Google Scholar
Qi WZ, Liu HH, Liu P, Dong ST, Zhao BQ, So HB, Li G, Liu H, Zhang JW, Zhao B (2012) Morphological and physiological characteristics of corn (
L.) roots from cultivars with different yield potentials. Eur J Agron 38:54–63.
https://doi.org/10.1016/j.eja.2011.12.003 CrossRef Google Scholar
Rajala A, Peltonen-Sainio P, Onnela M, Jackson M (2002) Effects of applying stem-shortening plant growth regulators to leaves on root elongation by seedlings of wheat, oat and barley: mediation by ethylene. Plant Growth Regul 38:51–59.
https://doi.org/10.1023/A:1020924307455 CrossRef Google Scholar
Sangoi L, Gracietti MA, Rampazzo C, Bianchetti P (2002) Response of Brazilian maize hybrids from different eras to changes in plant density. F Crop Res 79:39–51.
https://doi.org/10.1016/S0378-4290(02)00124-7 CrossRef Google Scholar
Schluttenhofer CM, Massa GD, Mitchell CA (2011) Use of uniconazole to control plant height for an industrial/pharmaceutical maize platform. Ind Crops Prod 33:720–726.
https://doi.org/10.1016/j.indcrop.2011.01.009 CrossRef Google Scholar
Sinica AA, Tshpa C (2007) Effects of population density on stalk lodging resistant mechanism and agronomic characteristics of maize. Electron Publ 33:1688–1695
Tokatlidis IS, Koutroubas SD (2004) A review of maize hybrids’ dependence on high plant populations and its implications for crop yield stability. F Crop Res 88:103–114.
https://doi.org/10.1016/j.fcr.2003.11.013 CrossRef Google Scholar
Tokatlidis IS, Has V, Melidis V, Has I, Mylonas I, Evgenidis G, Copandean A, Ninou E, Fasoula VA (2011) Maize hybrids less dependent on high plant densities improve resource-use efficiency in rainfed and irrigated conditions. F Crop Res 120:345–351.
https://doi.org/10.1016/j.fcr.2010.11.006 CrossRef Google Scholar
Tripathi SC, Sayre KD, Kaul JN, Narang RS (2003) Growth and morphology of spring wheat (
L.) culms and their association with lodging: Effects of genotypes, N levels and ethephon. F Crop Res 84:271–290.
https://doi.org/10.1016/S0378-4290(03)00095-9 CrossRef Google Scholar
Van Ittersum MK, Cassman KG (2013) Yield gap analysis and Rationale, methods and applications—Introduction to the Special Issue. F Crop Res 143:1–3
CrossRef Google Scholar
Vega CRC, Andrade FH, Sadras VO (2001a) Reproductive partitioning and seed set efficiency in soybean, sunflower and maize. F Crop Res 72:163–175.
https://doi.org/10.1016/S0378-4290(01)00172-1 CrossRef Google Scholar
Vega CRC, Andrade FH, Sadras VO, Uhart SA, Valentinuz OR (2001b) Seed number as a function of growth. A comparative study in soybean, sunflower, and maize. Crop Sci 41:748–754.
https://doi.org/10.2135/cropsci2001.413748x CrossRef Google Scholar
Wang Y, Li Q, Li C, Li A (1998) Studies on the culm quality and anatomy of wheat varieties. Acta Agron Sin 24:458–462
Wang C, Ruan R, Yuan X, Hu D, Yang H, Li Y, Yi Z (2014) Relationship between lignin metabolism and lodging resistance of culm in buckwheat. J Agric Sci 6:29–36.
https://doi.org/10.5539/jas.v6n9p29 Google Scholar
Wang C, Hu D, Liu X, She H, Ruan R, Yang H, Yi Z, Wu D (2015) Effects of uniconazole on the lignin metabolism and lodging resistance of culm in common buckwheat (
M.). F Crop Res 180:46–53.
https://doi.org/10.1016/j.fcr.2015.05.009 CrossRef Google Scholar
Xu C, Gao Y, Tian B, Ren J, Meng Q, Wang P (2017) Effects of EDAH, a novel plant growth regulator, on mechanical strength, stalk vascular bundles and grain yield of summer maize at high densities. F Crop Res 200:71–79.
https://doi.org/10.1016/j.fcr.2016.10.011 CrossRef Google Scholar
Xue J, Gou L, Zhao Y, Yao M, Yao H, Tian J, Zhang W (2016) Effects of light intensity within the canopy on maize lodging. F Crop Res 188:133–141.
https://doi.org/10.1016/j.fcr.2016.01.003 CrossRef Google Scholar
Yan Y, Gong W, Yang W, Wan Y, Chen X, Chen Z, Wang L (2010) Seed treatment with uniconazole powder improves soybean seedling growth under shading by corn in relay Strip intercropping system. Plant Product Sci 13:367–374.
https://doi.org/10.1626/pps.13.367 CrossRef Google Scholar
Yang SL, Zhang XN, Lu GL, Wang CR, Wang R (2015) Regulation of gibberellin on gene expressions related with the lignin biosynthesis in “Wangkumbae” pear (
Nakai) fruit. Plant Growth Regul 76:127–134.
https://doi.org/10.1007/s10725-014-9982-0 CrossRef Google Scholar
Zheng M, Chen J, Shi Y, Li Y, Yin Y, Yang D, Luo Y, Pang D, Xu X, Li W, Ni J, Wang Y, Wang Z, Li Y (2017) Manipulation of lignin metabolism by plant densities and its relationship with lodging resistance in wheat. Sci Rep 7:41805.
https://doi.org/10.1038/srep41805 CrossRef PubMed PubMedCentral Google Scholar Copyright information
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