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Methyl jasmonate alleviates arsenic-induced oxidative damage and modulates the ascorbate–glutathione cycle in oilseed rape roots

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Abstract

Methyl jasmonate (MJ) is an important plant growth regulator, involves in various physiological processes of plants. In the present study, role of MJ in tolerance to oilseed rape (Brassica napus L.) roots under arsenic (As) stress was investigated. The treatments were comprised of three MJ doses (0, 0.1, and 1 µM) and two levels of As (0 and 200 µM). Arsenic stress resulted in oxidative damage as evidenced by decreased root growth and enhanced reactive oxygen species and lipid peroxidation. However, plants treated with MJ decreased the H2O2 and O2 ·− contents in roots and have higher antioxidant activities. Importantly, results showed that MJ enhanced the redox states of AsA and GSH, and the related enzymes involved in the AsA–GSH cycle. Moreover, MJ also induced the secondary metabolites related enzymes (PAL and PPO) activities, under As stress. PAL and PPO expression was further increased by MJ application in the roots of B. napus under As stress. MJ also reduced the total As content compared with As alone treated plants. These findings suggest the role of MJ in mitigation of the As-induced oxidative damage by regulating AsA and GSH redox states and by reducing As uptake in both cultivars.

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Acknowledgements

This study was supported by the National High Technology Research and Development Program of China (2013AA103007), the Special Fund for Agro-scientific Research in the Public Interest (201303022), the National Natural Science Foundation of China (31570434, 31650110476), Jiangsu Collaborative Innovation Center for Modern Crop Production, and the Science and Technology Department of Zhejiang Province (2016C02050-8).

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Authors and Affiliations

  1. Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China

    Muhammad A. Farooq, Faisal Islam, Chong Yang, Rafaqat A. Gill, Basharat Ali & Weijun Zhou

  2. Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Muhammad A. Farooq & Habib-ur-Rehman Athar

  3. Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Aamir Nawaz

  4. Agricultural Technology Extension Center, Zhejiang University, Hangzhou, 310058, China

    Wenjian Song

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  1. Muhammad A. Farooq
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  2. Faisal Islam
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Correspondence to Wenjian Song or Weijun Zhou.

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Farooq, M.A., Islam, F., Yang, C. et al. Methyl jasmonate alleviates arsenic-induced oxidative damage and modulates the ascorbate–glutathione cycle in oilseed rape roots. Plant Growth Regul 84, 135–148 (2018). https://doi.org/10.1007/s10725-017-0327-7

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