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PEG-simulated drought stress and spike in vitro culture are used to study the impact of water stress on barley malt quality

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Abstract

Water deficient or drought stress is a major factor causing deterioration or instability of malt barley quality. In the studies on the influence of drought stress during grain filling on malt quality formation or metabolic changes, it is quite difficult to obtain the uniform plant individuals and water condition in pot or field experiments. In this study, we combined barley spike in vitro culture and PEG-6000 simulated drought to determine the genotypic difference in the changes of grain metabolites and the expression level of the genes encoding β-amylase and β-glucan using two Tibetan wild barley accessions and two cultivated genotypes differing in malt quality stability under drought stress. Under simulated drought, grain weight and β-glucan content were dramatically reduced and β-amylase activity was increased, and a lot of metabolites were markedly changed for all genotypes. On the whole, the changes were relatively smaller in the wild barley. Meanwhile, the expressions of Bmy1 related to β-amylase synthesis and GSL1, GSL4 and GSL7 related to β-glucan synthesis were up-regulated and down-regulated under drought stress, respectively, being consistent with the changes of β-amylase activity and β-glucan content in the four barley genotypes. The current results showed that PEG-6000 simulated drought and spike in intro culture may provide the basically similar information on grain development or metabolites as do in the field experiments, and it is suitable for use in studies on the influence of drought stress on quality traits during grain filling stage of barley or other cereal crops.

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References

  • Ahmed IM, Cao F, Han Y, Nadira UA, Zhang G, Wu F (2013) Differential changes in grain ultrastructure, amylase, protein and amino acid profiles between Tibetan wild and cultivated barleys under drought and salinity alone and combined stress. Food Chem 141:2743–2750

    Article  CAS  PubMed  Google Scholar 

  • Bamforth CW (1982) Barley β-glucans: their role in malting and brewing. Brewers Digest 3:22–35

    Google Scholar 

  • Christensen U, Scheller HV (2012) Regulation of (1,3;1,4)-β-d-glucan synthesis in developing endosperm of barley lys mutants. J Cereal Sci 55:69–76

    Article  CAS  Google Scholar 

  • Gous PW, Gilbert RG, Fox GP (2015) Drought-proofing barley (Hordeum vulgare) and its impact on grain quality: a review. J Inst Brewing 121:19–27

    Article  CAS  Google Scholar 

  • Hejgaard J, Boisen S (1980) High-lysine proteins in Hiproly barley breeding: Identification, nutritional significance and new screening methods. Hereditas 93:311–320

    Article  CAS  Google Scholar 

  • Jacobsen KS, Kalvenes C, Olsen O-A (1991) mRNA levels in the developing aleurone and starchy endosperm in wild type and a high lysine (lys 3a) mutant of barley. Physiol Plant 83:201–208

    Article  Google Scholar 

  • Jones CA, Jacobsen JS, Wraith JM (2005) Response of malt barley to phosphorus fertilization under drought conditions. J Plant Nutr 28:1605–1617

    Article  CAS  Google Scholar 

  • Kaufmann MR, Eckard AN (1971) Evaluation of water stress control with polyethylene glycol by analysis of guttation. Plant Physiol 47:454–456

    Article  Google Scholar 

  • Lanzinger A, Frank T, Reichenberger G, Herz M, Engel K (2015) Metabolite profiling of barley grain subjected to induced drought stress: responses of free amino acids in differently adapted cultivars. J Agri Food Chem 63:4252–4261

    Article  CAS  Google Scholar 

  • Lisec J, Schauer N, Kopka J, Willmitzer L, Fernie AR (2006) Gas chromatography mass spectrometry–based metabolite profiling in plants. Nat Protoc 1:387–396

    Article  CAS  PubMed  Google Scholar 

  • Luo JL, Tang SH, Peng XJ, Yan XH, Zeng XH, Li J, Li XF, Wu G (2015) Elucidation of cross-talk and specificity of early response mechanisms to salt and PEG-simulated drought stresses in brassica napus using comparative proteomic analysis. PLoS One 10:e0138974

    Article  PubMed  PubMed Central  Google Scholar 

  • Macnicol PK, Jacobsen JV, Keys MM, Stuart IM (1993) Effects of heat and water stress on malt quality and grain parameters of Schooner barley grown in cabinets. J Cereal Sci 18:61–68

    Article  CAS  Google Scholar 

  • McCleary BV, Codd R (1989) Measurement of β-amylase in cereal flours and commercial enzyme preparations. J Cereal Sci 9:17–33

    Article  CAS  Google Scholar 

  • McCleary BV, Codd R (1991) Measurement of (1/3), (1/4)-β-d-glucan in barley and oats: a streamlined enzymic procedure. J Sci Food Agr 55:303–312

    Article  CAS  Google Scholar 

  • Meng S, Zhang CX, Su L, Li YM, Zhao Z (2016) Nitrogen uptake and metabolism of Populus simonii in response to PEG-induced drought stress. Environ Exp Bot 123:78–87

    Article  CAS  Google Scholar 

  • Molina-Cano JL, Polo JP, Sopena A, Voltas J, Pérez-Vendrell AM, Romagosa I (2000) Mechanisms of malt extract development in barleys from different European regions II. Effect of barley hordein fractions on malt extract yield. J Inst Brew 106:117–123

    Article  CAS  Google Scholar 

  • Nam K, Kim D, Shin HJ, Nam KJ, An JH, Pack I, Park J, Jeong S, Kim HB, Kim C (2014) Drought stress-induced compositional changes in tolerant transgenic rice and its wild type. Food Chem 153:145–150

    Article  CAS  PubMed  Google Scholar 

  • Neumann PM (2008) Coping mechanisms for crop plants in drought-prone environments. Ann Bot 101:901–907

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ovesná J, Kučera L, Vaculová K, Štrymplová K, Svobodová I, Milella L (2012) Validation of the β-amy1 transcription profiling assay and selection of reference genes suited for a RT-qPCR assay in developing barley caryopsis. PLoS One 7:e41886

    Article  PubMed  PubMed Central  Google Scholar 

  • Qi JC, Zhang GP, Zhou MX (2006) Protein and hordein content in barley seeds as affected by nitrogen level and their relationship to beta-amylase activity. J Cereal Sci 43:102–107

    Article  CAS  Google Scholar 

  • Raggi V (1992) Changes in water relations and in some physiological functions of bean under very light osmotic shock-induced by polyethylene-glycol. Physiol. Planta 84:537–548

    Article  CAS  Google Scholar 

  • Richards FJ (1959) A flexible growth function for empirical use. J Exp Bot 10:290–300

    Article  Google Scholar 

  • Romagosa I, Prada D, Moralejo MA, Sopena A, Munoz P, Casas AM, Swanston JS, Molina-Cano JL (2001) Dormancy, ABA content and sensitivity of a barley mutant to ABA application during seed development and after ripening. J Exp Bot 52:1499–1506

    Article  CAS  PubMed  Google Scholar 

  • Savin R, Nicolas ME (1996) Effects of short periods of drought and high temperature on grain growth and starch accumulation of two malting barley cultivars. Funct Plant Biol 23:201–210

    Google Scholar 

  • Schober MS, Burton RA, Shirley NJ, Jacob AK, Fincher GB (2009) Analysis of the (1,3)-β-d-glucan synthase gene family of barley. Phytochemistry 70:713–720

    Article  CAS  PubMed  Google Scholar 

  • Sicher RC, Timlin D, Bailey B (2012) Responses of growth and primary metabolism of water-stressed barley roots to rehydration. J Plant Physiol 169:686–695

    Article  CAS  PubMed  Google Scholar 

  • Sørensen MB, Cameron-Mils V, Brandt A (1989) Transcriptional and post-transcriptional regulation of gene expression in developing barley endo-sperm. Mol Gen Genet 217:195–201

    Article  Google Scholar 

  • Thameur A, Ferchichi A, López-Carbonell M (2014) Involvement of abscisic acid metabolites and the oxidative status of barley genotypes in response to drought. Cana J. Plant Sci 94:1481–1490

    Article  CAS  Google Scholar 

  • Wallwork MAB, Logue SJ, MacLeod LC, Jenner CE (1998) Effects of a period of high temperature during grain filling on the grain growth characteristics and malting quality of three Australian malting barleys. Aus J Agr Res 49:1287–1296

    Article  Google Scholar 

  • Wei K, Jin XL, Chen X, Wu FB, Zhou WH, Qiu BY, Qiu L, Wang XD, Li CD, Zhang GP (2009) The effect of H2O2 and abscisic acid (ABA) interaction on β-amylase activity under osmotic stress during grain development in barley. Plant Physiol Biochem 47:778–784

    Article  CAS  PubMed  Google Scholar 

  • Wenzel A, Frank T, Reichenberger G, Herz M, Engel K (2015) Impact of induced drought stress on the metabolite profiles of barley grain. Metabolomics 11:454–467

    Article  CAS  Google Scholar 

  • Widodo PJH, Newbigin E, Tester M, Baci A, Roessner U (2009) Metabolic responses to salt stress of barley (Hordeum vulgare L.) cultivars, Sahara and Clipper, which differ in salinity tolerance. J Exp Bot 60:4089–4103

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu D, Cai S, Chen M, Ye L, Chen Z, Zhang H, Dai F, Wu F, Zhang G (2013) Tissue metabolic responses to salt stress in wild and cultivated barley. PLoS One 8:e55431

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu XJ, Chen X, Zeng FR, Zhang GP (2015) The genotypic difference in the effect of water stress after anthesis on the malt quality parameters in barley. J Cereal Sci 65:209–214

    Article  CAS  Google Scholar 

  • Yang JC, Zhang JH, Liu K, Wang ZQ, Liu LJ (2006) Abscisic acid and ethyleneinteract in wheat grains in response to soil drying during grain filling. New Phytol 171:293–303

    Article  CAS  PubMed  Google Scholar 

  • Zekri M (1991) Effects of peg-induced water-stress on 2 citrus cultivars. J Plant Nutr 14:59–74

    Article  Google Scholar 

  • Zhang G, Chen J, Wang J, Ding S (2001) Cultivar and environmental effects on (1→3,1→4)-β-d-glucan and protein content in malting barley. J Cereal Sci 34:295–301

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Natural Science Foundation of China (31330055, 31371559), the Fundamental Research Funds for the Central Universities (2014FZA6011) and Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP).

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  1. Zhejiang Key Lab of Crop Germplasm, Agronomy Department, Zhejiang University, Hangzhou, 310058, China

    Xiaojian Wu, Fanrong Zeng & Guoping Zhang

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  1. Xiaojian Wu
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  2. Fanrong Zeng
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  3. Guoping Zhang
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Correspondence to Guoping Zhang.

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Wu, X., Zeng, F. & Zhang, G. PEG-simulated drought stress and spike in vitro culture are used to study the impact of water stress on barley malt quality. Plant Growth Regul 81, 243–252 (2017). https://doi.org/10.1007/s10725-016-0201-z

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